Lazare Saladin

Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...

Excited to share my first PhD student’s @biorxivpreprint.bsky.social!

Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!

Check it out!

www.biorxiv.org/content/10.1...

A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...

Nanosensors for real-time intracellular analytics - Nature Nanotechnology This Review provides insights into nanosensor technologies for monitoring cellular biomarkers, proposing a spatial framework to clarify their distinct advantages, challenges and performance for high-r...

www.nature.com/articles/s41...

Bridging Light and Sound: a Spironaphtopyran-Rhodamine Dyad with High-Contrast Photoswitching Between Fluorescence and Photoacoustic Signal Fluorescence and photoacoustic imaging are complementary modalities that provide distinct advantages for biological imaging: fluorescence microscopy offers high sensitivity and resolution, while photo...

Happy to share our new preprint, a fantastic collaboration with @prevedel-lab.bsky.social ! We developped a probe that can switch between fluorescence and photoacoustic contrast upon illumination. Congrats to @kaydanych.bsky.social and Magda who led this project.
www.biorxiv.org/content/10.1...

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....

What makes PhD students happy? Good supervision Supervisors who invest in positive mentoring relationships with their PhD candidates also reap the benefits for their own research.

www.nature.com/articles/d41...

Home | Reisenbauer Lab The Reisenbauer Lab is a research group in biocatalysis and enzyme engineering at the IST Austria.

It’s finally happening!🎉 The Reisenbauer Lab will open in February at ISTA (www.reisenbauerlab.com
).
We’re starting to build our team and are looking for enthusiastic scientists to join us.

I feel deeply grateful to my mentors, colleagues, and family for all their support. Excited for what’s next!

What can HaloTag do for you?

Triggered fluorogenic labelling?
Molecular recording?
Photo-Chemically Induced Dimerisation?

Philipp Mauker's CHalo motif lets reagents do all three, and more.

Read the preprint here -
doi.org/10.1101/2025...

#chembio #fluorescence #cellbio #SLP

It's official, the Young French ChemBio group has just been launched 💫 !

Our mission ? To bring together the next generation of chemical biologists 🧪​🧬, foster exchange opportunities, and promote interdisciplinary science in France and beyond !

@scf-chembio.bsky.social

Open positions GR-SCHUHMACHER

We are hiring!

If you are looking for a postdoc position and you are interested in signaling lipids and proteomics, come join us in beautiful Switzerland!

More information on www.epfl.ch/labs/gr-schu...

In our new paper in JACS Au, with the Sagara group from the Institute of Science Tokyo, we introduce flippers than respond to stretching rather than compression and applicability to the materials rather than the life sciences (GOA).

pubs.acs.org/doi/10.1021/...

Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.

#PhDposition #OrganicChemistry #ChemBio - deadline 03.08 - please RT ❤️ Application online stellenangebote.uni-marburg.de/jobposting/d...

Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

New paper online:

Ångström-resolution imaging of cell-surface glycans.

The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.

#Glycotime

www.nature.com/articles/s41...

Check it out at PNAS today. Thank you Alexandre for the collaboration!!

PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!

Available @spirochrome.com

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

🚨 #ExM meets #eSRRF 🔬🏄🌊 the full protocol now in ‪@natprot.nature.com‬: doi.org/10.1038/s415...

🔬👨‍💻📰 #SReD is out!
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.

Check: www.nature.com/articles/s41...

Congrats to Yin-Hsi Lin and all the authors!

Preprint Link: www.biorxiv.org/content/10.1...

Great news: I received ERC Advanced grant #ERCAdG @erc.europa.eu CaptuRel! It aims at intelligent nanomaterials undergoing bioinspired cycle of capture and photorelease of bioactive molecules for sensing and controlling (bio)chemical gradients. Thanks to my Team, @cnrs.fr and @unistra.fr!

Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1...
Congratulations to Yin-Hsi Lin!

In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors Dopamine (DA) plays a crucial role in a variety of brain functions through intricate interactions with other neuromodulators and intracellular signaling pathways. However, studying these complex netwo...

Excited to share our Science paper on HaloDA1.0-the first genetically encoded far-red dopamine sensor! 🥳It enables powerful multiplex imaging in neurons, brain slices, and live animals.🧠🔬 #GRABsensors #Dopamine www.science.org/doi/10.1126/...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

If you always wanted to know the kinetics of how the Polycomb enzymes modify chromatin, we are thrilled to share our work!!! 🎉📄🧬🔬

Thank you, @beatfierz.bsky.social for all the guidance as well as the @lcbm-epfl.bsky.social group members for all the support!
doi.org/10.1126/scia...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established.
www.science.org/doi/10.1126/...

A palette of bridged bicycle-strengthened fluorophores - Nature Methods A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com

Read the News & Views highlighting this work, here!

www.nature.com/articles/s41...

Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET The correlation of individual conformational changes in dynamic protein complexes remains challenging as most structural methods rely on averaged information over a large number of molecules. Single molecule FRET is a powerful tool for monitoring such conformational changes. When performed using three distinct probes, it enables the correlation of domain movements by providing up to three simultaneous distance measurements with high temporal resolution. Nevertheless, a major challenge lies in the site-specific attachment of three probes to unique positions within the target protein. Here, we propose an orthogonal triple-labeling strategy that is not compromised by native, reactive amino acid functionalities. It combines genetic code expansion and bioorthogonal labeling of two different noncanonical amino acids with an enzymatic self-labeling SNAP tag. We demonstrate its application by establishment of a 3-color sensor on the human metabotropic glutamate receptor 2, a dimeric, multidomain G protein-coupled neuroreceptor, and describe a previously unknown conformational intermediate state using 3-color single molecule FRET.

Our new study is out !
We introduce a triple labeling strategy (2 non canonical Amino Acids + 2 click chemistries and 1 SNAP-tag) to triple label a G-protein coupled receptor.
Open access in JACS
pubs.acs.org/doi/10.1021/...