Proteios Technology

Imidazole vs. Lysine: How to Improve Lab Safety and Protein Integrity — Proteios Technology Imidazole may be compromising both lab safety and protein integrity. Discover why scientists are switching to lysine for a safer, more efficient protein purification process. Learn how lysine-based me...

Exploring safer purification strategies is an important step toward protecting both researchers and the environment, while still achieving high-quality results.

Imidazole vs. Lysine: How to Improve Lab Safety and Protein Integrity — Proteios Technology Imidazole may be compromising both lab safety and protein integrity. Discover why scientists are switching to lysine for a safer, more efficient protein purification process. Learn how lysine-based me...

As a result, many labs are shifting toward non-toxic alternatives like lysine-based purification and silica-based resins. These methods not only improve lab safety but also help maintain protein integrity and reduce hazardous waste.

Imidazole vs. Lysine: How to Improve Lab Safety and Protein Integrity — Proteios Technology Imidazole may be compromising both lab safety and protein integrity. Discover why scientists are switching to lysine for a safer, more efficient protein purification process. Learn how lysine-based me...

With a growing focus on safer lab practices and sustainability, researchers are rethinking the chemicals they use daily. Traditional protein purification methods often rely on imidazole and nickel-based systems, which come with toxicity risks and environmental concerns.

The AptaCapture is the future for cell therapy for #rarediseases, and that starts with innovation:
✅ 99% purity in cell isolation (vs. 95%)
✅ 30-minute isolation (vs. 150+ min)
✅ 7.5x cell expansion (vs. 5x)
✅ Lower exhaustion marker (PD1: 32%), improving long-term efficacy #RareDiseaseResearch

Cell therapy methods are slow, costly, and inefficient for #rarediseases. The Proteios AptaCapture Platform is here to fix that. By eliminating the need for antibodies & nanobeads, we’re making cell therapy faster, more scalable, and accessible for researchers & clinicians.

For #RareDiseaseDay we’re at the PNRI & Seattle Children's Rare Disease Symposium today, showcasing how Proteios Technology is transforming cell therapy manufacturing for rare diseases. Should be a great event for the Seattle rare disease community! #RareDiseaseDay #Seattle #PNRI

Today is #RareDiseaseDay a time to recognize the 300M+ people worldwide living with rare diseases. Many conditions, like AEC syndrome & rare B-cell cancers, lack effective treatments. Advancing research & innovative therapies is crucial.
#RareDiseaseAwareness #RDD2025 #medsky

Silica vs. Nickel in Protein Purification: Safer, Sustainable, and High-Performance Solutions — Proteios Technology Unlike nickel NTA agarose, which is toxic, flammable, and causes heavy metal contamination, Proteios’ silica-based resin is non-toxic, non-flammable, and biodegradable. Achieve high-yield protein puri...

Nickel resins are widely used in protein purification, yet they persist in the environment, bioaccumulate in ecosystems, and pose toxicity risks to researchers and aquatic life. Ethanol stabilization adds flammability concerns and increases hazardous waste disposal requirements. #nomorenickel

Silica vs. Nickel in Protein Purification: Safer, Sustainable, and High-Performance Solutions — Proteios Technology Unlike nickel NTA agarose, which is toxic, flammable, and causes heavy metal contamination, Proteios’ silica-based resin is non-toxic, non-flammable, and biodegradable. Achieve high-yield protein puri...

Want to know how silica outperforms nickel resins? We’ve got the data.

Check out our latest deep dive on sustainable protein purification. 🌱🔬

www.proteios.com/proteios-blo...

#Biotech #LabSafety #Sustainability #Silica #ProteinPurification

Silica vs. Nickel in Protein Purification: Safer, Sustainable, and High-Performance Solutions — Proteios Technology Unlike nickel NTA agarose, which is toxic, flammable, and causes heavy metal contamination, Proteios’ silica-based resin is non-toxic, non-flammable, and biodegradable. Achieve high-yield protein puri...

Nickel-based purification harms the planet with heavy metals & hazardous waste.
🌱 Silica is the greener choice:
✅ No toxic nickel—protects ecosystems
✅ No ethanol—reduces VOC emissions
✅ Sustainable disposal—no hazardous waste handling
Purify proteins, not the environment. #Sustainability 3/n

Silica vs. Nickel in Protein Purification: Safer, Sustainable, and High-Performance Solutions — Proteios Technology Unlike nickel NTA agarose, which is toxic, flammable, and causes heavy metal contamination, Proteios’ silica-based resin is non-toxic, non-flammable, and biodegradable. Achieve high-yield protein puri...

Nickel resins put researchers at risk, ditch the nickel, switch to silica for safer protein purification!

✅ Non-toxic—no heavy metals or health risks
✅ Non-flammable—no ethanol, no fire hazards
✅ Easy disposal—no hazardous waste protocols
Protect your team. Choose safer purification. 2/n

Silica vs. Nickel in Protein Purification: Safer, Sustainable, and High-Performance Solutions — Proteios Technology Unlike nickel NTA agarose, which is toxic, flammable, and causes heavy metal contamination, Proteios’ silica-based resin is non-toxic, non-flammable, and biodegradable. Achieve high-yield protein puri...

Nickel resins are common in protein purification, but at what cost?
⚠ Toxicity – Heavy metal exposure harms health & ecosystems.
🔥 Fire Risk – Ethanol-stabilized resins need strict safety measures.
🗑 High Costs – Hazardous waste disposal adds complexity.
Safer alternatives exist ⬇️ #NickelResin 1/n

Here’s what we found:
✔ KRAS, TEV protease, and PFK were purified with 85-95% purity.
✔ Yields reached up to 2mg in a single run.
✔ The workflow avoided toxic reagents and took less than 15 minutes.

The gel tells the story—clear, strong bands for some of the toughest-to-purify proteins!
3/3

Here’s what we found:
✔ KRAS, TEV protease, and PFK were purified with 85-95% purity.
✔ Yields reached up to 2mg in a single run.
✔ The workflow avoided toxic reagents and took less than 15 minutes.

The gel tells the story—clear, strong bands for some of the toughest-to-purify proteins!
3/3

To purify we used the RapidPro™ Spin Kit with a streamlined workflow: cell lysis, loading, washing, and elution—all in under 15 minutes.
Each protein faced unique challenges:
🔬 KRAS – medium-low expression in E. coli.
🔬 TEV protease – low solubility.
🔬 PFK – very low expression and yield.
2/n

KRAS, TEV protease, and T. thermophilus PFK are crucial for advancing research in cancer, molecular biology, and thermophilic bacteria. Low expression levels, solubility issues, and complex workflows make them difficult to purify, so we tested the RapidPro™ Spin Kit to overcome these issues 1/n

Hello Will!
Proteios Technology would love to be included in the Seattle Biotech Starter Pack!

✅ Achieved >90% protein purity <30 minutes—directly from crude lysates
✅Gravity columns eliminate the need for pumps or special equipment
✅ Effective for difficult-to-purify proteins, including GFPmut2, mCherry, and MBP, with purities between 80–90%
✅ Hazard-free—no imidazole needed (no toxic waste)

Transforming Protein Purification: The Innovation Behind the RapidPro™ Gravity Kit — Proteios Technology Protein purification is the backbone of breakthroughs in biochemistry, structural biology, and therapeutic development. Yet, traditional methods often create bottlenecks, demanding multiple steps, cos...

Traditional protein purification is often slow, costly, and complex. At Proteios Technology, we tested the RapidPro™ Gravity Kit to see if it could simplify workflows while maintaining precision. The results? Game-changing.
Learn More: www.proteios.com/proteios-blo...

✅ Achieved >90% protein purity <30 minutes—directly from crude lysates
✅Gravity columns eliminate the need for pumps or special equipment
✅ Effective for difficult-to-purify proteins, including GFPmut2, mCherry, and MBP, with purities between 80–90%
✅ Hazard-free—no imidazole needed (no toxic waste)

Transforming Protein Purification: The Innovation Behind the RapidPro™ Gravity Kit — Proteios Technology Protein purification is the backbone of breakthroughs in biochemistry, structural biology, and therapeutic development. Yet, traditional methods often create bottlenecks, demanding multiple steps, cos...

Traditional protein purification is often slow, costly, and complex. At Proteios Technology, we tested the RapidPro™ Gravity Kit to see if it could simplify workflows while maintaining precision. The results? Game-changing.
Learn More: www.proteios.com/proteios-blo...

New year, new me-thods! 🧪✨ Time to leave inefficient purifications in 2024 where they belong. Here’s to higher yields, cleaner proteins, and fewer lab tantrums in 2025. 💧🔬

RapidPro Gravity™ — Proteios Technology Discover RapidPro Gravity™ kits: Simplifying protein purification with gravity force. Enjoy effortless, high-quality protein collection sans complex setups!

Traditional methods for protein purification often require multiple chromatography steps, costly reagents, and specialized equipment. We’ve streamlined this with the RapidPro Gravity™ Column, which leverages the Car9 tag to simplify and enhance protein purification through a gravity flow system.

What’s the most challenging part of your research process? A) Protein purification B) Data analysis C) Grant writing D) Other (tell us below!)

Read about how the #RapidPro Kits enable purification of low to moderate expressed recombinant proteins. Using the Proteios #Car9 affinity tag, proteins like KRAS, TEV, and PFK were purified in under 15 minutes with 85-95% purity, without toxic metal ions, achieving up to 2mg per column. 5/5

The #Car9 tag revolutionized protein purification by achieving over 98% purity and high yields for high expressed proteins like GLK, MBP, and sfGFP. In under 30 minutes, the #Car9 tag efficiently purified with yields up to 8mg per 50mL culture, without the need for toxic reagents or metal ions.⬇️4/5

Car9 Tag: A Breakthrough in Metalloprotein Purification — Proteios Technology The 38th Protein Society Symposium, held at the Westin Hotel in Vancouver, BC, was a hub of cutting-edge research in protein science, attracting researchers and innovators from around the globe. Among...

Several case studies highlight its success in purifying highly expressed proteins, oncogene proteins, transcription factors, and #metalloproteins. Using non-toxic, metal-free resins, the #Car9 tag maintains protein integrity and functionality, making it an essential tool for all applications. ⬇️3/5

Proteios History- What does Car9 mean, anyway? — Proteios Technology Proteios had its origin back in 2015.&nbsp; I was working with researchers at the University of Washington to identify if their technology had commercial potential and to assist them in applying for an initial grant from the University to perform the analysis.&nbsp; In one project, I worked

We’re focused on revolutionizing protein purification with the #Car9 tag (DSARGFKKPGKR), a silica-binding peptide. The #Car9 tag enables rapid, high-purity #recombinant #protein purification without the use of toxic reagents. ⬇️2/5
www.proteios.com/proteios-blo...

Hey BlueSky! 👋Proteios Technology here! We’re leveraging advances in protein engineering and gene synthesis to offer a brand-new recombinant protein purification method that’s rapid, simple, and safe. 🧪✨
Here is a brief introduction to our recent work! ⬇️ 1/5