Adrian Liston

Thank you editors for improving the readability of our paper by removing acronyms. Now we grow our cluster of differentiation 4+ Forkhead box 3+ cells in Roswell Park Memorial Institute media with phorbol 12-myristate 13-acetate.

TIL, RPMI media is actually Roswell Park Memorial Institute media

An education in tolerance: the 2025 Nobel Prize in Physiology or Medicine

journals.biologists.com/dmm/article/...

How can we optimize the delivery of IL-2 to traumatic brain injury patients? Published in Expert Opinion on Drug Delivery (Ahead of Print, 2025)

How can we optimize the delivery of IL-2 to traumatic brain injury patients?

www.tandfonline.com/doi/10.1080/...

Ah, I thought you were advocating for multi-member seats. Single member seats with STV are a good innovation.

PR is a more democratic system than FPTP, but it is not the only option, and is one which causes polarisation and fragmentation. Why not push for STV instead, which is also more democratic and rewards consensus candidates?

Das Bild zeigt Adrian Liston, James Dooley und Oliver T. Burton von der Cambridge Universität, wie sie grünes Fairy-Spülmittel in einen Erlenmeyerkolben mit blauer Flüssigkeit geben. Rechts daneben steht die Überschrift des im Post angekündigten Artikels „Färbereagenz mit Spülmittel.“ Es folgt dessen Vorspann mit dem Text: „Die Durchflusszytometrie erfordert eine feine Balance zwischen Fixierung der Zellen und Durchlässigkeit der Zellmembran. Am einfachsten gelingt dies mit Geschirrspülmittel.”

Adrian Liston, James Dooley und Oliver T. Burton von der Cambridge Universität nutzen Fairy-Spülmittel zur Entwicklung eines optimierten Puffers für die Durchflusszytometrie. Mehr im Artikel von Andrea Pitzschke
👉 www.laborjournal.de/editorials/3...
@campathology.bsky.social
@labliston.bsky.social

Introducing the Dish Soap Protocol: A Unified Approach for Multi‐Modal Intracellular Staining Recent advances in dyes and cytometers have seen an exponential increase in the ability to perform multidimensional flow cytometry. As we increase our capacity to extract information from cells, the ...

@labliston.bsky.social shared a paper they had written using dish soap as a permeabiliser for Flow Cytometry:

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Someone I work with tried it, and it had the additional benefit of reducing Autofluorescence, which will give you better data!

How can we optimize the delivery of IL-2 to traumatic brain injury patients? Published in Expert Opinion on Drug Delivery (Ahead of Print, 2025)

How can we optimize the delivery of IL-2 to traumatic brain injury patients?

www.tandfonline.com/doi/10.1080/...

Image shows a circular glowing intersex flag behind three lit candles. Text reads: Intersex Day of Remembrance

November 8th marks Intersex Day of Remembrance.

Intersex Day of Remembrance is a day to remember those who are no longer with us and highlight issues that the Intersex community face.

We stand in solidarity with our Intersex siblings, team members and supporters!

#IntersexDayOfRemembrance

It’s dishwashing liquid ;)

That would be amazing!

I love a good recipe 💚✨️

Fairy Liquid unveiled as secret ingredient in University of Cambridge scientists’ near-universal magic formula It helps them with experiments studying the inside of cells.

It is not often that a new flow cytometry protocol makes the newspaper! I also got a kick out of seeing our science tagged as "quirky"!

www.cambridgeindependent.co.uk/news/fairy-l...

Cautious or Protesting, Foreign Scholars Skip U.S. Conferences As President Donald Trump deploys National Guard troops to American cities and ICE arrests pick up, some international scholars are opting out of U.S. travel.

Foreign Scholars Skip US Conferences

“I don’t agree with the politicization of scientific funding, the destruction of data collection and openness... and I feel like traveling to America at this point is a partial endorsement of what’s going on,” Liston said.

www.insidehighered.com/news/faculty...

Cautious or Protesting, Foreign Scholars Skip U.S. Conferences As President Donald Trump deploys National Guard troops to American cities and ICE arrests pick up, some international scholars are opting out of U.S. travel.

Foreign Scholars Skip US Conferences

“I don’t agree with the politicization of scientific funding, the destruction of data collection and openness... and I feel like traveling to America at this point is a partial endorsement of what’s going on,” Liston said.

www.insidehighered.com/news/faculty...

Yes, I agree completely! Technical advances need experience. It isn't a coincidence that the first author here is a permanent staff scientist with a decade in the lab.

Different lab structures have different strengths and weaknesses. This is the bonus for large and stable labs :)

Fascinating talk tonight from Malala Yousafzai

🧪

As the editor of CSH Protocols, I wholeheartedly agree.

And if you have an idea for a topic to do a protocols collection on, or know of a field that would benefit from such a collection, let's talk!

Haven't read this one yet, but looks like a fantastic resource for everyone working with complex #spectral #flowcytometry panels!

AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level The advent of spectral flow cytometry has seen a rapid rise in the complexity of flow cytometry experiments, allowing the construction of assays with at least 50 fluorescent parameters. To correctly d...

A small 🧵 encouraging people to publish protocols.

Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.

www.biorxiv.org/content/10.1...

With so many new methods buried in results-focused papers, this thread is my encouragement to groups to also publish separate protocols papers! You'll be pleasantly surprised at the engagement these papers get, and most importantly they provide real impact aiding others in their science.

A cross entropy test allows quantitative statistical comparison of t-SNE and UMAP representations Dimensionality-reduction tools such as t-SNE and UMAP allow visualizations of single-cell datasets. Roca et al. develop and validate the cross entropy test for robust comparison of dimensionality-redu...

Our cross entropy test was probably the first statistical test allowing the comparison of tSNE/UMAPs. It helped groups understand that dimensionality reduction can be used for more than simply visualisation.

www.cell.com/cell-reports...

AutoSpill is a principled framework that simplifies the analysis of multichromatic flow cytometry data - Nature Communications Flow cytometry allows the simultaneous quantification of many markers in and on a cell, but the analysis of such data is complicated. Here, the authors propose AutoSpill, a framework that facilitates ...

You might not know about #AutoSpill, but if you do flow cytometry you've almost certainly used it. This improvement to compensation calculation has been integrated into all major flow cytometry programs, such as FlowJo, reducing error for the whole community

www.nature.com/articles/s41...

Optimization of the Blocking and Signal Preservation Protocol in High‐Parameter Flow Cytometry High quality input data is the key to successful interpretation of any scientific assay. In flow cytometry, fluorescently-conjugated antibodies allow us to simultaneously measure an incredible range ...

This paper did the hard-yards of testing various different blocking and signal preservation protocols. For any flow cytometry researchers a thorough read could save months of troubleshooting.

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Introducing the Dish Soap Protocol: A Unified Approach for Multi‐Modal Intracellular Staining Recent advances in dyes and cytometers have seen an exponential increase in the ability to perform multidimensional flow cytometry. As we increase our capacity to extract information from cells, the ...

Our "Fairy dishwashing" protocol is just out, but is already gaining traction as a cheap and effective way to stain for both transcription factors and cytokines. Work that needed reporter strains can now rapidly be done via flow.

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Do more with Less: Improving High Parameter Cytometry Through Overnight Staining Recent advances in flow cytometry have allowed high-dimensional characterization of biological phenomena, enabling breakthroughs in a multitude of fields. Despite the appreciation of the unique prope....

For example, our overnight staining protocol might not get cited often, but it has nearly 40,000 downloads. It reduced our antibody costs nearly 100-fold. That is a lot of funding available for extra research!

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...