Bryan Bryson

Congrats to the Carpenter Lab on this exciting study!

I might now sound like a broken record, but check the abstract!!!

Let’s go type 7 secretion system substrates! #tuberculosis #tbsky

Happy to see more data pointing to an important role for type 7 secretion substrates in TB protective immunity

Original paper: https://rupress.org/jem/article/222/10/e20250444/278135/Targeting-infection-specific-peptides-in Video summary by Owen Leddy (Massachusetts Institute of Technology) Description: One… Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery

New video summary 🎥 from Leddy et al. @thebrysonlab.bsky.social (rupress.org/jem/article/... ) on a new workflow called PathMHC that helps find pathogen-derived antigens presented on MHCs by infected cells that could be targets for T cell priming vaccines:

Congrats to the amazing Owen Leddy for this tour de force which was inspired by reviewer comments from our previous paper and thanks to our amazing friends and collaborators for making the project a reality.

And thanks to JEM for a very pleasant and efficient review process!

Importantly, for Mtb, the results are consistent with our previous study (doi.org/10.7554/eLif...) where we showed that Mtb-derived peptides on MHC-I are enriched for type VII secretion system substrates underscoring our enthusiasm for including these antigens in TB vaccines.

Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery MS-based immunopeptidomics can facilitate vaccine antigen discovery, but self-MHC peptides greatly outnumber pathogen-derived MHC peptides, presenting a ch

Newest paper from the lab out today (doi.org/10.1084/jem....)! Here, we describe PathMHC, a novel MS-based approach to identify rare pathogen-derived peptides on MHC-I and MHC-II. We apply this to a range of pathogens Mtb, SARS-CoV-2, and Listeria.

Anyone have experience with IV mRNA-LNP vaccination in animals? I am specifically interested in LNP formulations and what the preferred formulation for IV is.

Pictured: glass of champagne I treated myself to at DCA upon learning the lab has been tenured!

Grateful to my lab family, my extended science family, and my family for the amazing support and love offered on the journey so far.

Much more work left to do but taking this moment to say thank you!

Does anyone know of a CRO that produces MVA-based vaccines?

Do any of my #tbsky friends have interests in T cell responses in people with evidence of prior Mtb infection?

If so, would you have any interest in looking at T cell responses to Mtb-derived peptides that we have detected by mass spectrometry? LMK 🙏🏽

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling The mycobacterial cell envelope plays both infectious and protective roles. Understanding its structure is crucial for unlocking the molecular basis underlying these functions. Studying glycans, the p...

First post here! To inaugurate this account, I'd like to share my recent work, now published in JACS.

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Thank you!

Does anyone have a favorite protocol for in vitro priming of human T cells from PBMCs? #immunosky

So glad to see this out! I learned a lot in this project about how to think creatively about customizing our approach to using language models for immune receptors

Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?

Before I write my own analysis script, does anyone in #tbsky have a tool for pathway analysis for Mtb genes?

A lovely day to celebrate the amazing people I work with every day

Yes! Can work with that!

Thanks! Would love any suggestions!

Hadn’t seen this — thank you — will read!

Omg! This is the best news — congratulations Chuck!!!

Any of y’all doing expansion on bacteria specifically those that are historically difficult to permeability? If so, I have a bacterium for you ;-)

What is your favorite cytosolic pathogen that (a) infects macrophages and (b) is genetically tractable?

Looking for someone who’d like to team up on a small but impactful project (we would do the heavy lifting!)

He’s been very helpful for our project!

Would love to chat sometime — have been thinking about innate cells and bacteria!

My whole scientific identity over the last 96 hours has been species differences in immunity. Would love to connect with others on this topic!

Anyone on here love cathepsins or other lysosomal proteases? Would love to pick your brain for 15 minutes!

Oh heyyyyyy!!!

Anyone want to discuss T cell vaccines? I’ve been in a rabbit hole lately and would love to discuss with others!

I made a Starter Pack for the Myco & TB community.
Hope to find more good friends here on Bluesky to keep in touch about mycobacterial infections. Please join and repost!

go.bsky.app/3nwpcVV