SapKrap

Allscripts? I'm not sure. This is very complex software that has no forgiveness for failure in a very regulated environment. Brent Shafer basically destroyed Cerner through contempt for human capital and this was compounded by the people from Oracle. I don't think old Cerner is recoverable.

I'm reliant on 1.2/1.3 300 mesh; any deviations I can't really work around. I know SubAngstrom was making 1.8/2 300 mesh some time back, I wonder if they're making other sizes now. Also, Single Particle LLC offered good pricing for ANTcryos last I asked, maybe it would be worth getting some...

Same experience! I did get some trial packs from Mitegen but they were fragile and required optimization beyond my patience. Lots of torn squares but I did get some nice particles.

I had something growing in a SDS stock solution years back and I just put it in the back of a drawer. If you want to grow in a 10% surfactant solution you deserve respect.

Scoop: Janet Mills to launch Senate bid Tuesday, per internal document Mills' entrance into the race is also likely to set off a divisive primary contest.

She would be 79 if elected. I don't know what victory Chuck thinks he has here.
Not since the 1920s or Reconstruction has the Democratic party had such sclerotic and weak leadership.
www.axios.com/2025/10/09/u...

A protein tunnel that shuttles lipids around the cell The high-resolution structure of a ‘superhighway’ for lipids in cells has been obtained.

www.nature.com/articles/d41...

Never knew you could use worms for expression!

Absolutely appalling, so sorry to hear this Jason.

My 4 y/o told me something no parent ever wants to hear. "I want to be a protein scientist with a microscope like you".
He's too young to understand you could be a C elegans geneticist or an architect and not someone that spends weeks freezing aggregates on rare metal disks.

RFK Jr. confirmed, elevating anti-vaccine activist to nation’s top health post The Senate held a final vote on whether to approve Robert F. Kennedy Jr. for Health and Human Services secretary after a three-month confirmation fight.

What a stupid country

www.washingtonpost.com/health/2025/...

Dem Overseeing 'Digital Assets' to Hold Luxury Retreat With Crypto Kingpin Arizona Sen. Ruben Gallego, who’s overseeing a “digital assets” committee, is set to hold a luxury weekend donor retreat with a crypto kingpin.

This is really such a hopeless political party.

www.rollingstone.com/politics/pol...

I should have been clearer in my reply, I was referring to the paper in my post. I'm not a prolific author at all, but I have had demands where it was clear that nothing was enough for the reviewers. It appears that Mignot and Cambillau didn't have such demands from Nature for more experiments.

AF has been of use at a gross level for reconstructions, but for my projects the predictions have been rather poor. I don't know enough about the subject matter in the paper, but if I were to publish something similar I would only feel comfortable with complimentary experiments to prove my points.

Structural model of a bacterial focal adhesion complex - Communications Biology The structural model of a bacterial focal adhesion complex reveals the connection of essential functional modules throughout the cell envelope and offer an inspiring view of the force transduction mec...

www.nature.com/articles/s42...

This is the first "all AlphaFold and nothing else" paper I've seen

Old Outlook: Click calendar entry->open attachment
New Outlook: Click calendar entry->opens browser window->organization SSO->opens Office 365 calendar -> click calendar entry -> you do not have permission to open this file

If you're still there, Maru Maru in Papeete was a major highlight for us

I was there last year, can't wait to go back.

Moorea?

Congratulations Brandon!

Every time JD Vance talks I feel an uncomfortable hum in my ears.

Combining cryoEM and functional studies we found that mammalian TRPM5 exhibit unique properties and are uniquely sensitive to calcium. Coupling of MHR domains seems to influence gating, but we'll need more TRPM structures to determine this.

Very happy to show the first work published from the Zubcevic Lab at KUMC.
www.pnas.org/doi/10.1073/...

An entire day wasted because of Unicorn 7.1! I don't know why Cytiva needs to make the most cumbersome software. I've spoken to people at dozens of institutions who like Aktas but have hated the Unicorn install.

In my hometown of Springfield, MO for the first time in a long time and the fattest man I've ever seen is wearing a shirt with an AR-15 on it that says "Socialism Preventer"

Never thought I would say this, but Phenix on Windows has not crashed once this project whereas Phenix on my Ubuntu box can only run from command line.

www.nature.com/articles/s41...

What a delightful structural paper, so happy to see it published

From Andrew Fire, Viroid-like colonists of human microbiomes
www.biorxiv.org/content/10.1...

AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structu... An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...

www.nature.com/articles/s41...

Half a year wait for Quantifoils. Outrageous that wait times have never improved, only gotten worse. Has anyone ever used C-flats? Are they about the same or worse?

Sometimes I have real apprehension about the future of experimental structural biology. The predictions for my proteins of interest are currently poor and some domains are nonsensically arranged. Who is to say this couldn't change in ten, maybe five years though?