Beuzón & Ruiz-Albert Lab

For those who might have missed:
#RNAsky
(We really need a ribosome emoji at this point)

Oxygen levels can affect how resistance genes work, impacting antibiotic efficacy. This can be relevant for the most used ones: for example, resistance to fosfomycin varies with oxygen.

Learn more in our latest paper 👉 bit.ly/3T8sdpp a work led by @jaescudero.bsky.social

#AntibioticResistance

Dan from my lab presented his work on Pseudomonas phage that has an intriguing role in virulence. #planthealth2025 #proudPI

A man giving a scientific presentation with a PowerPoint slide showing phage presence across bacteria genomes

Daniel Maddock (@michhulin.bsky.social’s group) investigates prophage carrying a key effector protein that enables Pseudomonas bacteria to infect cherry trees.

Deleting the whole prophage reduces virulence of the bacterium on the plant (leaf and woody tissue) 🍒
#PlantHealth2025

First records of Aulacaspis tubercularis, Icerya seychellarum and Protopulvinaria pyriformis on Persea indica Persea indica (Lauraceae) is an endemic evergreen tree of the Macaronesian Laurisilva forests, valued for its ecological importance, ornamental appeal and bioactive compounds. We report, for the firs....

Just published!!🚨 New records: Persea indica found hosting novel scale insects. These pests, Aulacaspis tubercularis, Icerya seychellarum, and Protopulvinaria pyriformis, pose potential risks to native Macaronesian flora.
#PlantHealth #Entomology #Laurisilva
🔗 onlinelibrary.wiley.com/doi/10.1111/...

Congratulations Jose, let this one be the first of many, eager to collaborate with you!

🎉 Thrilled to announce that CoBEPP — Using conserved bacterial effectors to probe plant-pathogen interactions — has been funded by @ageinves.bsky.social & @cienciagob.bsky.social!
Grateful for the support to launch my first project @ihsmumacsic.bsky.social , exploring effector functions! 🌱🔬

Within-host bacterial evolution and the emergence of pathogenicity - Nature Microbiology In this Review, Tonkin-Hill et al. discuss the processes driving bacterial evolution and emergence of pathogenesis within hosts, the importance of understanding within-host genetic diversity, and the ...

#Review

A discussion on the processes driving bacterial evolution and emergence of pathogenesis within hosts, the importance of understanding within-host genetic diversity, and the implications for transmission analysis and infectious disease control.

#MicroSky 🦠

www.nature.com/articles/s41...

PSA on links: On Bluesky, there’s no limit to the number of links you can share, and they will receive the same visibility as your other posts 💙

We are on the lookout!

It is a strange thing how those can hold so many memories, right? Cindy, you had a great run, enjoy your retirement! Anyway, you cannot fool us, we are sure you are gonna keep going all sciency!

Congratulations everyone

Thanks Suzana!

miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis miR825-5p down-regulates immunity against Pseudomonas syringae and mediates cleavage of the sequence encoding the conserved motif within the MIST1 Toll/int

The collaboration was established on the basis of our common interests in molecular mechanisms of plant defence and it's self-regulation via miRNAs

doi.org/10.1093/jxb/...

This was a very pleasant collaboration! We wish the very best to Isabel on her retirement at the end of July: it has been an honour to collaborate with you and your team!

miR825‐5p‐regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae MicroRNAs are essential regulators in plant resistance to biotic stresses, but their specific roles in the plant–herbivore context require deeper investigation. Here, we studied how the Arabidopsis .....

And now for something completely different!

Very proud to have contributed to this work on molecular plant-pest interaction led by Isabel Diaz Rodriguez at CBGP

doi.org/10.1111/nph....

@cbgpmadrid.bsky.social
@ihsmumacsic.bsky.social
#PlantScience

As a bonus, this one comes with an always welcomed predoc contract! We'll be looking for PhD candidates soon...

Very grateful to @ageinves.bsky.social we'll try and get the most out of those funds!

@type3lab.bsky.social keeps going strong!

We have just been awarded a Project Grant (PID2024) from the Spanish Agencia Estatal de Investigación on the topic "Cooperative virulence in plants: mechanisms, dynamics and adaptive impact"

Congratulations everyone, a great July harvest at @ihsmumacsic.bsky.social

📢 IHSM La Mayora – New Publications in July!
We are pleased to share in this thread the scientific contributions from researchers at IHSM La Mayora published during the month of July.

🔬 Stay tuned for more updates on our research and collaborations.

Fig. 8. Scheme showing the potential influence of putrescine and spermidine biosynthesis on pathogenesis-related bacterial traits. (1) Biofilm production, important to resist harsh conditions found at the plant surface, seems to rely on an increase in putrescine production, whereas spermidine synthesis exerts a negative effect. However, both polyamines appear to have a significant positive influence on the capability of Pto to control (2) stomata aperture, (4) the production of pyoverdine and, therefore, the acquisition of iron and (5) the translocation of effectors into the host cell. (3) However, swimming motility seems to depend primarily on putrescine synthesis.

The influence of the polyamine synthesis pathways on Pseudomonas syringae virulence and plant interaction. Published #OpenAccess and fee-free in #MicrobioJ using a #PublishAndRead agreement:
doi.org/10.1099/mic....

Great job! Congratulations Alberto and team

Our latest article is now online!
@PlantBiotechnologyJournal

Expression of Arabidopsis FLS2 in N. benthamiana confers responsiveness to Ralstonia flg22 and activation of anti-bacterial immunity
doi.org/10.1111/pbi....

By Meng Li, Yujiao Chen, and Yali Wei

Editor's Pick: Shen, Ye et al. review recent literature on the role of bacterial ribosome heterogeneity on facilitating rapid response to stress.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

𝐏𝐆𝐑𝐏𝟐𝟎𝟐𝟔 - 𝐌𝐚́𝐥𝐚𝐠𝐚
Posttranscriptional gene regulation in Plants 🌱🧬
🗺️ Beautiful city of Málaga (Spain)
🗓️ 5-7 Oct 2026

Organizers:
@manavellalab.bsky.social
@kmerchante.bsky.social
@laura-ah.bsky.social

More info: very soon!

A mismatch between slow protein synthesis and fast environmental fluctuations determines tradeoffs in bacterial proteome allocation strategies https://www.biorxiv.org/content/10.1101/2025.07.22.666192v1

Temperate phages enhance host fitness via RNA-guided flagellar remodeling Bacterial flagella drive motility and chemotaxis while also playing critical roles in host-pathogen interactions, as their oligomeric subunit, flagellin, is specifically recognized by the mammalian immune system and flagellotropic bacteriophages. We recently discovered a family of phage-encoded, RNA-guided transcription factors known as TldR that regulate flagellin expression. However, the biological significance for this regulation, particularly in the context of host fitness, remained unknown. By focusing on a human clinical Enterobacter isolate that encodes a Flagellin Remodeling prophage (FRφ), here we show that FRφ exploits the combined action of TldR and its flagellin isoform to dramatically alter the flagellar composition and phenotypic properties of its host. This transformation has striking biological consequences, enhancing bacterial motility and mammalian immune evasion, and structural studies by cryo-EM of host- and prophage-encoded filaments reveal distinct architectures underlying these physiological changes. Moreover, we find that FRφ improves colonization in the murine gut, illustrating the beneficial effect of prophage-mediated flagellar remodeling in a host-associated environment. Remarkably, flagellin-regulating TldR homologs emerged multiple times independently, further highlighting the strong selective pressures that drove evolution of RNA-guided flagellin control. Collectively, our results reveal how RNA-guided transcription factors emerged in a parallel evolutionary path to CRISPR-Cas and were co-opted by phages to remodel the flagellar apparatus and enhance host fitness. ### Competing Interest Statement M.W.G.W. is a co-founder of Can9 Bioengineering. S.H.S. is a co-founder and scientific advisor to Dahlia Biosciences, a scientific advisor to CrisprBits and Prime Medicine, and an equity holder in Dahlia Biosciences and CrisprBits. A patent application has been filed related to this work (PCT Patent Application No. PCT/US24/40027). The remaining authors declare no competing interests. National Science Foundation, https://ror.org/021nxhr62, DGE-2036197, CAREER 2239685 National Institutes of Health, NIH DP2AI177904, R01CA259634, U24GM129539 Spain Ministry of Science Innovation and Universities Grant, PID2023-147463NB-I00 Simons Foundation, SF349247 Kinship Foundation Pew Biomedical Scholarship Irma T. Hirschl Career Scientist Award Howard Hughes Medical Institute Columbia University Irving Medical Center Dean’s Office Vagelos Precision Medicine Fund

Temperate phages enhance host fitness via RNA-guided flagellar remodeling | bioRxiv https://www.biorxiv.org/content/10.1101/2025.07.22.666180v1

New @ISMPMI Board Members #DreamTeam 😍 🇬🇧 🇨🇳 🇨🇦 🇦🇷 🇩🇪 🇰🇪

Two more articles in our series on 'Scientific mobility in microbiology' are online now.

Read @edelplopez.bsky.social and @tatsuyanobori.bsky.social's mobility-related experiences and their insights here: authors.elsevier.com/a/1lTnY,L%7E... & authors.elsevier.com/a/1lTnY_,2Ci...