Sagar Bashyal @sagarbashyal

It’s one of the best feelings 🙃

04.08.2025 21:36 — 👍 1 🔁 0 💬 0 📌 0

Congratulations Natalia 🎉

02.08.2025 02:23 — 👍 0 🔁 0 💬 1 📌 0

I am thrilled to share the 2nd chapter of my PhD with @adsteinbrenner.bsky.social In collaboration with @tiszapatrick we demonstrated that the LRR-RLP INR is the missing molecular link between caterpillar recognition and predatory wasp recruitment in the field. A thread 1/6

01.08.2025 21:25 — 👍 30 🔁 15 💬 2 📌 1

Unlocking expanded flagellin perception through rational receptor engineering - Nature Plants Receptor kinase FLS2 detects the flg22 epitope of bacterial flagellin. Here the authors identify key residues on FLS2’s concave surface that enable expanded perception of flg22 variants, allowing the ...

Excited this paper is finally published! We focused on the inner concave surface of LRR RKs to expand bacterial flagellin perception in plants. Selection indicates expanded perception is more common than previously thought. Experiments led by @jerrytli.bsky.social www.nature.com/articles/s41...

29.07.2025 01:20 — 👍 98 🔁 44 💬 10 📌 1

Grateful to be at #2025ISMPMI! Reconnected with my former mentor @carogutj.bsky.social and met Maria, my current PIs mentor-so many cool stories🌱
Loved the energy at the poster session and great chats, including with Ertao Wang whose AMF work continues to inspire. #AMF

15.07.2025 17:05 — 👍 5 🔁 2 💬 0 📌 0

Mark Estelle- UC San Diego- honored for his lifetime of excellence in research, mentorship, and support of the Arabidopsis community #ICAR2025

16.06.2025 19:05 — 👍 31 🔁 7 💬 0 📌 0

The latest publication from our lab!

The famous "glomalin" from arbuscular mycorrhizal (AM) fungi is not a protein but a polysaccharide from AM fungi, so we renamed it "glomalose". Glomalin-related proteins are bacterial proteins stuck in this glomalose.
nph.onlinelibrary.wiley.com/doi/10.1111/...

06.06.2025 13:54 — 👍 72 🔁 40 💬 3 📌 0

Exploring the untapped potential of single‐cell and spatial omics in plant biology Advances in single-cell and spatial omics technologies have revolutionised biology by revealing the diverse molecular states of individual cells and their spatial organization within tissues. The fie...

Excited to share my new Tansley Review @newphyt.bsky.social on emerging single-cell and spatial omics technologies, many of which are just beginning to be applied in plant biology! Big opportunities lie ahead for the field. [1/n]

nph.onlinelibrary.wiley.com/doi/10.1111/...

22.05.2025 06:46 — 👍 108 🔁 61 💬 3 📌 1

Plant Peptide Receptor Meeting 2025

Join us for the 🪴 🌵 Plant Peptide Receptor Meeting 2025 ☘️ 🪴 in 🏴󠁧󠁢󠁳󠁣󠁴󠁿 Edinburgh 🏴󠁧󠁢󠁳󠁣󠁴󠁿 8th-10th September

#plantsignaling #PlantScience #plantsci

pprm2025.events.kdmeventsportal.co.uk
@pprm2025.bsky.social

A thread 🧵
(1/n)

20.05.2025 14:43 — 👍 28 🔁 27 💬 1 📌 0

PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Congratulations Michael and the entire team!👏Cool work from the @carogutj.bsky.social lab and a testament to all the long hard work behind it @pnas.org . Grateful to have contributed to this story✌️. Checkout the mechanisms by which the RAM1 functions.
www.pnas.org/doi/full/10....

19.05.2025 20:37 — 👍 4 🔁 1 💬 0 📌 0

Thank you☘️

14.05.2025 01:42 — 👍 2 🔁 0 💬 0 📌 0

Our editors write:

- "Symbiosis signalling: Mycorrhizal CLE mimicry" rdcu.be/elQ6S

13.05.2025 12:53 — 👍 10 🔁 2 💬 2 📌 1

Mycorrhizal CLE mimicry - Nature Plants Nature Plants - Mycorrhizal CLE mimicry

Great to see our work highlighted in
@natplants.nature.com !🍀☘️🍄
Check it out: nature.com/articles/s41...
@lmueller.bsky.social @salkinstitute.bsky.social @ucsandiego.bsky.social

14.05.2025 01:31 — 👍 4 🔁 0 💬 0 📌 0

🌍 International Molecular Mycorrhiza Meeting 2025!
#IMMM2025
🏨 Book your hotel at our discounted group rate: genetik.bio.lmu.de/immm-2025/lo...
⚠️ Discounted rate only available until May 16th – act fast!

13.05.2025 12:00 — 👍 4 🔁 3 💬 0 📌 0

Signaling peptides control beneficial and pathogenic plant-microbe interactions Interactions between organisms, such as those between plants and microbes, require extensive signaling between and within each organism to detect and recognize the partner and elicit an appropriate response. Multiple families of small signaling peptides regulate plant interactions with beneficial or pathogenic microbes, and sometimes both. Some of these signaling peptides transmit information between different cells or organs of the host and allow plants to orchestrate a coordinated response towards microbial mutualists or pathogens. However, not only plants produce signaling peptides required for the interactions. Microbes themselves also secrete peptide signals, which are detected by host receptors and required for infection. Among these are microbial peptides mimicking those of plants, allowing mutualistic or pathogenic microbes to hijack endogenous plant signaling pathways and evade the host immune system. In this review, we provide a comprehensive summary of current knowledge on host- and microbe-derived signaling peptides and their cognate receptors regulating mutualistic and parasitic plant-microbe interactions. Furthermore, we describe how microbes hijack endogenous host signaling pathways, and discuss possible crosstalk between the plant signaling pathways controlling mutualism with those modulating immune responses to pathogens.

Great review from @lmueller.bsky.social -> Signaling peptides control beneficial and pathogenic plant-microbe interactions | Journal of Experimental Botany | Oxford Academic

09.05.2025 14:16 — 👍 11 🔁 6 💬 0 📌 0

Bacterial community-emitted volatiles regulate Arabidopsis growth and root architecture in a distinct manner from those from individual strains Short summary: This study reveals that the effects of bacterial community-produced volatiles on Arabidopsis growth and root architecture are an emergent property beyond the sum of effects by individua...

Finally, I am thrilled to share our long-term volatile project is out in Plant Communications! 🥳

Bacterial community-emitted volatiles regulate Arabidopsis growth and root architecture in a distinct manner from those from individual strains: Plant Communications www.cell.com/plant-commun...

07.05.2025 11:51 — 👍 30 🔁 11 💬 3 📌 1

Figure 1 from Bascom et al JEXBOT comparing the arfa-septuple mutant's growth to that of WT, tir1/afb-quad, and iaa2-degron. The arfa mutant's phenotype is strong but less severe than those of the tir1/afb and iaa2 mutants.

The second new paper from the Estelle lab out this week describes the phenotype of moss lines lacking all seven Class-A ARFs (Activating ARFs) and was lead former postdoc Carlisle Bascom, Jr.
academic.oup.com/jxb/advance-...

05.05.2025 22:39 — 👍 32 🔁 12 💬 4 📌 0

this could be to make a ready to use inoculum and yes they hardly mention the time

29.04.2025 20:06 — 👍 2 🔁 0 💬 1 📌 0

Seems like they were 6 months old....

29.04.2025 20:04 — 👍 2 🔁 0 💬 1 📌 0

Postdoctoral Researcher- Brady Lab University of California, Davis is hiring. Apply now!

We have a postdoc position that just opened up in the Brady lab on reprogramming tomato root system architecture in response to changes in nutrient availability - please consider applying! recruit.ucdavis.edu/JPF07095

19.04.2025 14:15 — 👍 96 🔁 109 💬 1 📌 2

The final version of our study on SERK-BIR module in Marchantia is out.

Congratulations Cate and many thanks to collaborators!

www.sciencedirect.com/science/arti...

17.04.2025 15:23 — 👍 52 🔁 27 💬 7 📌 1

Graphic abstract

🚨Preprint Alert!
Scooped by two other groups due to our lack of infrastructure to grow Arabidopsis, this study started in 2021, is still a great addition to the body of work looking for clubroot resistance in Arabidopsis! 💛
Link: doi.org/10.1101/2025...

16.04.2025 16:33 — 👍 36 🔁 10 💬 1 📌 0

Thank you Jean for the highlight☘️

16.04.2025 19:41 — 👍 1 🔁 0 💬 0 📌 0

A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides have emerged as key regulators of plant–microbe interactions, including arbuscular mycorrhizal (AM) symbiosis. Here, we identify Medicago truncatula CLE16 as a positive regulator of AM symbiosis. MtCLE16 is expressed in root cells colonized by AM fungi (AMF) and its overexpression within colonized tissues increases arbuscule abundance by finetuning their growth and lifespan. Functional and transcriptomic analyses reveal that MtCLE16 acts via the M. truncatula pseudokinase CORYNE (MtCRN) and suppresses the accumulation of reactive oxygen species (ROS) in roots, thereby attenuating immune responses and promoting root colonization by mutualistic AM fungi. Notably, AMF also express MtCLE16-like peptides. We show that the Rhizophagus irregularis MtCLE16-like peptide, RiCLE1, also attenuates ROS and promotes AMF colonization via MtCRN. This finding suggests that RiCLE1 can interfere with the MtCLE16-MtCRN signaling module of host roots to benefit the fungus. Our research uncovers a functional mechanism underpinning cross-kingdom signaling and molecular mimicry in mutualistic plant–microbe interactions.

Great paper on CLE peptides in AM symbiosis from @sagarbashyal.bsky.social, @lmueller.bsky.social and colleagues -> A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression

16.04.2025 18:30 — 👍 2 🔁 1 💬 1 📌 0

A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression | PNAS CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides have emerged as key regulators of plant–microbe interactions, including arbuscular mycorr...

www.pnas.org/doi/10.1073/...

15.04.2025 05:07 — 👍 11 🔁 9 💬 0 📌 0

A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression | PNAS CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides have emerged as key regulators of plant–microbe interactions, including arbuscular mycorr...

📜 A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN- mediated ROS suppression

🧑‍🔬 @sagarbashyal.bsky.social, @lmueller.bsky.social, et al.

📔 @pnas.org

🔗 pnas.org/doi/10.1073/...

#️⃣ #PlantScience #PlantSymbiosis #PlantDevelopment #PlantMycorrhiza

16.04.2025 07:34 — 👍 13 🔁 6 💬 0 📌 0

Thank you 🙏

15.04.2025 21:41 — 👍 1 🔁 0 💬 0 📌 0

Delighted to share this protocol with the scientific community.
This is a follow up to the article: www.sciencedirect.com/science/arti...

@lmueller.bsky.social
Marco Bürger
Joanne Chory

Link: star-protocols.cell.com/protocols/4142

15.04.2025 16:29 — 👍 0 🔁 0 💬 0 📌 0

Yes. This is likely a path we think.

15.04.2025 15:31 — 👍 1 🔁 0 💬 0 📌 0

Thank you Mary 🙏

15.04.2025 15:05 — 👍 2 🔁 0 💬 0 📌 0

Sagar Bashyal

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