Felipe Opazo

🧪🧬 New preprint!
What if the same biosensor could reveal how cysteine is regulated across life domains?

We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.

www.biorxiv.org/content/10.6...

🧵A thread ⬇️

This (and other slugs able to do this) inspired photosynthetic skin for humans!! www.symbiox.org/technology/, paper: www.symbiox.org/wp-content/u...

Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social

Let us know if your computer survived the cleaning process 😉. Mine will need this.

We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners.
Here’s to what’s next 🥂

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that the...

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Link ot he @biorxivpreprint.bsky.social is here: www.biorxiv.org/content/10.6...

Fantastic teamwork: @samratb.bsky.social, Kaushik Inamdar, Yoav Pollack, Laszlo Albert, Daniel Jans, Stefan Jakobs, @joergenderlein.bsky.social and @romantsukanov.bsky.social

JF635b-nanobodies are now commercially available via @nanotag.bsky.social.

✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Microtubule Organizing Centers of America (MTOCsA), sorry, not funny.

Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪!
Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨

#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.

Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!

See you, Christopher, in Rainiy Göttingen!

🦙 NanoTag at #SingleDomainAntibodies Conference 2025!

Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.

Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.

#ExM turned 10 this year - just like #NanoTag! 🎉
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.

See you in Göttingen!
#GEF25

Sounds great! Send tips, I am invited to the #MBSJ2025 in Tokyo the first week of December. Very much looking forward to it!

A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2 - Nature A neutralizing nanobody specific to the prefusion conformation of herpes simplex virus glycoprotein B has cross-species activity and offers insights into virus neutralization, possible immunogens and ...

Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...

🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth!
It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy.

See you next year! 🦙

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...

🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens.
Check full text: doi.org/10.1038/s414...

- A postdoc to work on cancer extravasation (4-year project).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).

🌞🦙 Summer makeover? You bet.
Our alpacas ditched the fleece to stay cool – maybe your antibodies should too.
sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more.
Browse our #nanobodies: nano-tag.com/shop/

💬Thank you, Dr. Maja Klevanski @majaklevanski.bsky.social (University of Heidelberg), for the great feedback on our recombinant anti-SARS-CoV-2 S1 antibody (rHcAb)! 😊 🔬 Applied in #dSTORM to study virion–cell interactions under antiviral conditions. Learn more about sdAb–Fc fusions 👉 bit.ly/3xdhYsn

🤹‍♀️ Still juggling primary antibodies from different species?
Still stuck in sequential staining?

⏱️Switch to Smart Secondaries®🦙:
✅OneStep-IF
✅Same-species #multiplexing
✅Higher precision & reproducibility!

Choose smart 👉 bit.ly/3UZxRMI

#SecondaryNanobody #Microscopy #Immunofluorescence

Yes, it might be that in some synaptic type, other synaptotagmin isoforms are more dominant, but it is far from demonstrated that there is NO Syt1 in such synapses...

A Versatile Synaptotagmin‐1 Nanobody Provides Perturbation‐Free Live Synaptic Imaging And Low Linkage‐Error in Super‐Resolution Microscopy Selection, characterization, and development of a nanobody that strongly and selectively binds the C2A domain of synaptotagmin-1, providing unparalleled imaging accuracy in various super-resolution i...

Try our anti-Synaptotagmin1 #nanobody, then you label endogenous targets without adding extra copies of anything synaptic. We can send you the AAV plasmids. Check paper here: onlinelibrary.wiley.com/doi/10.1002/...

@abberior.rocks — and NanoTag Biotechnologies rocks with them! 🤘

Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩

#SecondaryNanobodies
#SuperResolution
#FluorescenceMicroscopy

Congrats, nice work! Clever use of the ALFA system!