Matyas Bubna-Litic

Job alert! 📣 I’m looking for a research assistant to join my new team @idrm.ox.ac.uk

Were using #zebrafish to understand gene-environment interactions that shape the heart 🫀generate natural diversity 🐸🐭 and contribute to congenital defects ❤️‍🩹

Full info below, and please share! 🫶🏻

bit.ly/467TO0M

Excited to share Alison's @alisonkickuth.bsky.social paper from the lab out in @nature.com this week! We uncovered how a mechanical ratchet mechanism drives cytokinesis in early #zebrafish embryos. Read more in this thread 🧵 and at www.nature.com/articles/s41... 🤩
@poldresden.bsky.social @mpi-cbg.de

My group will investigate the stochastic morphodynamics of living systems at the interface of biophysical modelling and computational biology.

I will announce positions for PhD students and postdocs in the coming months! Feel free to DM me if you are interested!

Many small photos taken from the symposium organised to reveal Spemann and Mangold in the background.

We're in love with this cover image! 🧡
Part II of our Special Collection celebrating the Centennial of the Discovery of The Organiser is here. This issue focuses on the work presented at the Centennial Symposium at the University of Freiburg in September 2024:
www.sciencedirect.com/journal/cell...

#Mastodon, the Command Center for Large-Scale Lineage-Tracing Microscopy Datasets, is finally heading for publication (well, let's see). To #cite or not to cite shall no longer be the question 🙃, because the #preprint. Software is available in every #Fiji near you.

www.biorxiv.org/content/10.6...

Pleased to say that Jonas Hartmann (bs-less) and I have finally released DySTrack (“diss track”) - Dynamic Sample Tracking.

It’s a Python-based, modular tool that brings smart microscopy to everyday imaging on commercial systems.

Preprint: www.biorxiv.org/content/10.6...

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🌈 Rainbow neurons on the move!🧠
MultiColor FlpOut of L3 optic lobe neurons projecting into the medulla neuropil. 📸 Image by Isabel Holguera.
#FluorescenceFriday #DevBio

Thanks! 🦓🐟 are great for that

A figure illustrating examples of physics-inspired theory in developmental biology. Please read the linked article for more information.

Searching for physical principles of morphogenesis

In this #OpenAccess Spotlight, Nikolas Claussen, Fridtjof Brauns and @streichan.bsky.social highlight the ‘physics of development’, focusing on the interplay between quantitative experiments and mathematical theory

doi.org/10.1242/dev....

Fluorescent microscopy image of a 28 hours post fertilization zebrafish labelled with DAPI and phalloidin

Happy (and spooky) #FluorescenceFriday

Switching from frogs (which are underrated btw) to zebrafish has really made me appreciate transparent tissues! 🐟

28 hpf, ⚪ nuclei, 🔴 F-actin

📣Our project, “Mechanometabolic Control of Vertebrate Limb Elongation,” has been funded by Wellcome!
Over the next 8 years, we’ll collaborate with @manningresearch.bsky.social and Nathalie Agar’s group to understand the mechanics of ECM-rich mesenchymal tissues! Funded positions available!🎉

That's such a great book cover

Cartoon of a speculative heterogenous cell population performing mesenchymal collective cell migration.

1/12 A very special moment for me! 🎉

My first paper as corresponding author—a @jcb.org Perspective on how contact-based decision-making in collective cell migration can itself encode blueprints for complex patterns and shapes.

rupress.org/jcb/article/...

#cellbio #devbio #science

🧵for details

Animal caps are back in fashion!

Pretty cool

Schematic overview of our project. Dissection of Xenopus neural crest following morpholino knockdown of miRNA, then extraction of RNA for RNA-seq

Our new paper is out in Dev Biol! 🎉 🐸🧬
We show #miR-196a directs #neural crest fate in #Xenopus by repressing immature neural #ectoderm — evidence #miRNAs can potentially drive, not just fine-tune, early patterning
👉 Read more here: doi.org/10.1016/j.yd...
@devbiol.bsky.social @biouea.bsky.social

Mouse (Mus musculus) 🐭 is the powerhouse of mammalian genetics, essential for studying embryogenesis, organ development and gene function using transgenic technologies. 📸 Image by Zhong Hua also featured on the cover of Developmental Biology textbook (11th edition) #ModelMonday

Thank you for sharing! I'd like to highlight the concept of embryonic 'actuation' where a mechanical state change is caused by cells acting on each other through mechanics and/or biochemical signals but importantly not mediated by fate transitions.

Got stunning fluorescence microscopy images? 🌈🔬 SDB wants to feature YOUR work for #FluorescenceFriday! We highlight images from SDB members with a short description and the image creators 📸. Fill out the form to be featured: docs.google.com/forms/d/e/1F...

I'm going to be advertising for a few positions in my group in the next couple of months for a big project on synthetic microbial communities.

I would love to hear from you if you are interested and I'd also appreciate if everyone could share this far and wide!

✨Not a rainbow trout… a rainbow zebrafish 🌈 🐟
The expression of 10 different genes simultaneously including Hox genes along the body axis, somites, brain regions, skeletal muscle, and heart 🔬Image by Alice Sherrard, Gabby Jerz & Nipam Patel 🧪 #FluorescenceFriday

🚨Only 4 days to apply!!!🚨Join the SDB SciComm Internship! Gain hands-on experience writing stories on the latest developmental biology discoveries🔬Connect with top mentors & earn $500 to attend the SDB Annual Meeting 🎉! 👉 www.sdbonline.org/science_comm... #SciComm #DevBio

Postdoctoral Research Associate: Modelling Neural Crest Anomalies (mouse/human) - the Node The Liu lab is looking for a postdoc with a love of embryos and developmental biology! This post is part of an MRC-funded collaboration between Karen Liu’s lab at King’s College London and Marco Trizz...

Dev biologists! 3-yr postdoc position at King's, collaboration with Trizzino lab @marcotrizzino.bsky.social modelling CHD3 neural crest. We are looking for postdocs with mad embryology skills @the-node.bsky.social #developmental @ccrb27.bsky.social

thenode.biologists.com/jobs/postdoc...

This worked for me:
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.

Use: After permeabilising apply AF-reducing agent overnight at 4˚C

It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.

Hope it works!

Looks like an exciting meeting. Would you know if it's possible to attend online? Thanks

I'm not sure how bacteria on oysters are but in early Xenopus cells sodium borohydride works well to remove autofluorescence from sources like PFA fixation. There's a good protocol on CSH I can try look for it.

Got stunning fluorescence microscopy images? 🌈🔬 SDB wants to feature YOUR work for #FluorescenceFriday! We highlight images from SDB members with a short description and the image creators 📸. Fill out the form to be featured: docs.google.com/forms/d/e/1F...

Interesting review about the role of ECM in development!

We are searching for an enthusiastic scientist to join our team and establish annual killifish as a model system for early developmental biology and biophysics! @embl.org @embldbunit.bsky.social

Apply here:
embl.wd103.myworkdayjobs.com/EMBL/job/Hei...

Please re-post 🙏

Mechanochemical coupling of cell shape and organ function optimizes heart size and contractile efficiency in zebrafish Andrews et al. demonstrate that multiscale feedback between mechanical and chemical cues builds a functional heart to support zebrafish embryonic life. Cell recruitment and organ-scale forces drive tr...

Thrilled to bits to see our latest work online in Dev Cell! 🥳

We wanted to know how cells build functional organs with precision🫀🫁📏 Here we show how coupling of cell shape and organ function fine tunes the form and contractile power of the developing #zebrafish heart 1/n

tinyurl.com/cell-stretch