Wim Hagen

Finally, an FSC discussion again! It’s been a while…..

Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

Last day of a fantastic Inserm tomography workshop in Bordeaux.

Oranjerie Roermond tonight.

Cold showers and warm beer the rest of the week at CCPEM!

Mark? @subangstrom.bsky.social

Ever wondered how some of the automation in cryo-EM data collection works? Read Bram Koster's 1989 thesis!

repository.tudelft.nl/record/uuid:...

I like the genre!

Take a look at my personal perspective on the past, present, and future of in situ structural biology with cryo-ET+

While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all

www.cambridge.org/core/journal...

Looking for a primer on why the contrast transfer function matters in #cryoEM?

Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...

Phase plates: what do we expect from the next attempt, the laser phase plate?

As long as it’s not past 2/3 Nyquist, nobody cares.