Misha Kudryashev

Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin.

It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend

application.mdc-berlin.de/site/index.p...

It's a team effort of the group: Igor, Ulja, Xiaofeng, Artsemi, Patricia, and we thank the funders.

The print version will come up in a book on methods in molecular biology, hopefully later this year!

Enjoy reading and have a great week!

But for a larger system, smaller proteins, interactions with the cytoskeleton, cluster formation, and other biological applications, there seems to be a use of this microvesicle technique.

Membrane protein isolation and structure determination in cell-derived membrane vesicles - PubMed Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of pro...

Some thoughts: wonderful papers by Roderick Mackinon and other groups show that 2D imaging of membrane proteins in small vesicles can result in high-resolution structures. So if you want structures - try that!

pubmed.ncbi.nlm.nih.gov/37098056/

The book chapter is a set of methods to express your membrane protein of interest, purify vesicles, image them by cryo-ET, and perform subtomogram averaging.

As usual, we like to go into details:

The chapter is inspired by our recent paper, where we show that tetrameric and pentameric forms of the serotonin receptor ion channel 5-HT3R were present in the same membrane patches
www.embopress.org/doi/full/10....

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography

zenodo.org/records/1717...

Roses are red
Because they have SCREP!

Lithium deficiency and the onset of Alzheimer’s disease - Nature Lithium has an essential role in the brain and is deficient early in Alzheimer’s disease, which can be recapitulated in mice and treated with a novel lithium salt that restores the physiological level...

I'd say it's a paper of the week if not a year

Lithium deficiency and the onset of Alzheimer’s disease
www.nature.com/articles/s41...

Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇

For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!

www.mdc-berlin.de/postdocs#t-g...

Building atomic models to cryo-EM maps of huge molecules is intimidating: crowded, uncertain copy numbers, variable resolution ...
Fan Liu and team incl us, show how cross-linking MS can be used to build novel models of unknown complexes, like a giant Melbourne virus
biorxiv.org/content/10.1...

Thanks Mimi! ❄️❄️❄️

Thanks Sandip!

Thanks John !!!

We have a preprint for you

EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer

By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

It's fellowship season in the EU. If you want to talk and join our group at the amazing MDC in Berlin on a fellowship, please write me an email.

We do cryo-EM/ET, membrane proteins, data science/AI, our work: scholar.google.com/citations?so...

Fellowships: Marie Curie, EMBO, HFSP, Humboldt...

Our contribution to Mohit Misra, Ivan Đikić, and Co's stellar research on how a pathogenic bacterium, Legionella pneumophila, hijacks host cell signaling

It was a huge effort and took many years to combine all the data and methods, so enjoy reading👇
www.researchsquare.com/article/rs-6...

I think that it is not compatible with Krios 5

yeah, its very sad that Thermo makes older devices non-compatible.

Thanks!

Thanks !

Cool thanks Paulo. Any pros cons on arctis vs hydra ?

Cool thanks Katie, we have a similar experience in house. It's a great working horse 🐴🐴🐴

Hydra does look good on paper. Anything that could be improved in it ?

Thanks ! Cool. Are you happy with the milling throughput ?

Hi Cryo-EM fellas!

What are the pros and cons of the latest cryoFIB milling tools? Any usage experience with Arctis already? Any huge advantages of plasma?

Haha thanks !!! Text if you know someone!!

A broad call for a PhD researcher/ postdoc in our group at the MDC in Berlin. An ideal PhD student - a computational scientist, a postdoc - a structural biologist, but feel free to re-convince me!

We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...

Sooo, does anyone know the actual specs of the newly announced Thermo Fischer Krios G5?

Marketing material is wonderful, but what does it actually do ? 😁

PhD /Postdoc in In Situ Structural Biology by Cryo-ET Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.

It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...

English version of the documentary about power abuse in the Max Planck Society can be found here. 144.000 views already, more than 800 comments. Share it to make colleagues aware of what happens in German academia & the MPG! #IchBinHanna #PowerAbuse