A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function - Nature Communications Here they combine in vivo imaging with proteomics to characterize a protein complex comprised of Ccdc78 and Ccdc33 that acts at the extreme distal tip of 9 + 2 motile cilia to control tip architecture...

Cilia alert! Stoked to have the new paper from Juyeon Hong about a new domain at the extreme distal tip of motile cilia! (The EDT, y'all!) It's out now @natcomms.nature.com.
Check it out!
#cilia

www.nature.com/articles/s41...

CiliaBuilder Tutorials - YouTube CiliaBuilder Tutorials

The entire video list with Installation, Build Cilia, Centriole & 2D template

www.youtube.com/playlist?lis...

YouTube video by Bui Lab ChimeraX CiliaBuilder - Modelling Cilia Basics

CiliaBuilder Video Tutorial
www.youtube.com/watch?v=SJHo...

If your installation didn't work a few days ago, try again. I've fixed a few things, and let me know if it still doesn't work for you.

I just noticed that I did not put the proper dependency. Now, if you download version 1.0.6, it will install the dependency properly to run.

I am planning to do the next release with ArtiaX style cilia in the near future :) You probably will like that a lot more.

This was built in ~10 days of vibe-coding with Claude & Gemini. Now officially on holiday 😌

Video tutorial coming after the holidays

Tired of drawing cilia or centrioles for slides and papers? I feel you.

Check out CiliaBuilder for ChimeraX → cxtoolshed.rbvi.ucsf.edu/apps/chimera...

Build custom 3D cilia & centriole models (tips + primary cilia included).

We also analysed SPEF1-KO cilia by cryo-ET to show that SPEF1 restricts C1 and C2 microtubule movement.

In the final version, we included a lot more data and analysis compared to the previous biorxiv version. We achieved a 4.6 Angstrom resolution of the microtubule by subtomogram averaging of MT-SPEF1 complexes.

Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1 Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The cil…

Our newest paper is now online in Current Biology. In short, we identified 7 proteins in the tip of the cilia and found SPEF1 is a seam-binding protein and important for cilia stability. Great work from my postdoc Thibault Legal and everyone involved!

www.sciencedirect.com/science/arti...

This seems great. I want to working on our picking using AI in the summer. Perhaps, I can ask you for an installation and usage guide if I have problems.

I hated Amira with the $$. Need to try DragonFly but most of our processing is done on the cluster so anything using command line is a big advantage. Perhaps, my summer project :)

interesting I want to use dragonfly for segmentation but can we run dragonfly in command line for batch mode? Do you have a script to cluster and turn dragon fly into filament light?

Very nice preprint from the Wallingford lab. Seems like there is a common theme of tip proteins as these proteins seem to be in the tip of the ciliate cilia too.

Thanks. I found template matching is too slow for my need as I can manual pick microtubules much faster and more accurate. These tryout takes time so I 'd love to see people's experiences.

#TeamTomo What is the open-source and good cryoET deep-picking program?

Cryo-electron tomography of Tetrahymena #cilia by Sam Li, David Agard, Mark Winey provides insights into the intricate spatial organization of basal body inner junctions, compromised by loss of Poc1
www.embopress.org/doi/full/10....

Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination Eukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia structures rely on intraflagellar transport (IFT), which ...

We have posted our new #preprint regarding the track recognition mechanism of dynein-2 that works in #cilia on bioRxiv @biorxivpreprint.bsky.social!!
www.biorxiv.org/content/10.1...
1/8 🧵

DNAHX: a novel, non-motile dynein heavy chain subfamily, identified by cryo-EM endogenously Ciliogenesis and cilia motility rely on the coordinated actions of diverse dyneins, yet the complexity of these motor proteins in cilia has posed challenges for understanding their specific roles. Tra...

Excited to share our work on bioRxiv before my thesis defense! Together with my undergraduate mentee Diego, postdocs Wan and Jun, and my advisor Kai, we identified a novel dynein heavy chain subfamily, DNAHX, from sea urchin sperm axoneme.

Check out the details here: www.biorxiv.org/content/10.1...

Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social

www.nature.com/articles/s41...

Amazing work. It is so useful for us working with Tetrahymena and for cilia in general.

I am excited to share some of my PhD work on the motile cilia interactome revealed by XL/MS with @jbwallingford.bsky.social @edwardmarcotte.bsky.social Ophelia Papoulas, Chanjae Lee, David Taylor, and @builab.bsky.social www.cell.com/developmenta...

ChimeraX 1.9 has been released and is available now on our website

Thanks. Warp on Linux is awesome. This is my Twitter import so it is very old tweet

My last post on Twitter before.

Proudly present our new preprint on my first post here. We performed SPA on the ciliary tip and identify 7 proteins. Among them, we found the conserved protein SPEF1 at the microtubule seam. In vitro assays show that SPEF1 binds to the seam and stablizes microtubules.
www.biorxiv.org/content/10.1...

The Bui lab Twitter will stop and we are migrating to Mastodon. Anyway, the last post is to thank the @Beck_Laboratory and @jankosinski @aobarska for the new wall art decoration. NPC rules :

Our paper on the T- and N-pilus structure with the @ChristianBaron and @NatalieZeytuni labs is finally online. Great work from Amro Jaafar and @TheRealCSBlack and others.

authors.elsevier.com/a/1gElW3SNvc2%…

Taking forever but finally here. We identified close to 40 MIPs in Tetrahymena doublet. Specifically, the MIPs at the outer junction (CFAP77, OJ2 & OJ3). @dynein_awk @TheRealCSBlack @ahmad__khalifa

Just update the subtomo2Chimera withto visualize subtomo from Dynamo. Also, small fixes for relionsubtomo2ChimeraX. now you can also using stl file for visualization for more efficient memory #TeamTomo

github.com/builab/subtomo… dynamotable2ChimeraX.py