We also analysed SPEF1-KO cilia by cryo-ET to show that SPEF1 restricts C1 and C2 microtubule movement.

In the final version, we included a lot more data and analysis compared to the previous biorxiv version. We achieved a 4.6 Angstrom resolution of the microtubule by subtomogram averaging of MT-SPEF1 complexes.

Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1 Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The cil…

Our newest paper is now online in Current Biology. In short, we identified 7 proteins in the tip of the cilia and found SPEF1 is a seam-binding protein and important for cilia stability. Great work from my postdoc Thibault Legal and everyone involved!

www.sciencedirect.com/science/arti...

This seems great. I want to working on our picking using AI in the summer. Perhaps, I can ask you for an installation and usage guide if I have problems.

I hated Amira with the $$. Need to try DragonFly but most of our processing is done on the cluster so anything using command line is a big advantage. Perhaps, my summer project :)

interesting I want to use dragonfly for segmentation but can we run dragonfly in command line for batch mode? Do you have a script to cluster and turn dragon fly into filament light?

Very nice preprint from the Wallingford lab. Seems like there is a common theme of tip proteins as these proteins seem to be in the tip of the ciliate cilia too.

Thanks. I found template matching is too slow for my need as I can manual pick microtubules much faster and more accurate. These tryout takes time so I 'd love to see people's experiences.

#TeamTomo What is the open-source and good cryoET deep-picking program?

Cryo-electron tomography of Tetrahymena #cilia by Sam Li, David Agard, Mark Winey provides insights into the intricate spatial organization of basal body inner junctions, compromised by loss of Poc1
www.embopress.org/doi/full/10....

Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination Eukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia structures rely on intraflagellar transport (IFT), which ...

We have posted our new #preprint regarding the track recognition mechanism of dynein-2 that works in #cilia on bioRxiv @biorxivpreprint.bsky.social!!
www.biorxiv.org/content/10.1...
1/8 🧵

DNAHX: a novel, non-motile dynein heavy chain subfamily, identified by cryo-EM endogenously Ciliogenesis and cilia motility rely on the coordinated actions of diverse dyneins, yet the complexity of these motor proteins in cilia has posed challenges for understanding their specific roles. Tra...

Excited to share our work on bioRxiv before my thesis defense! Together with my undergraduate mentee Diego, postdocs Wan and Jun, and my advisor Kai, we identified a novel dynein heavy chain subfamily, DNAHX, from sea urchin sperm axoneme.

Check out the details here: www.biorxiv.org/content/10.1...

Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social

www.nature.com/articles/s41...

Amazing work. It is so useful for us working with Tetrahymena and for cilia in general.

I am excited to share some of my PhD work on the motile cilia interactome revealed by XL/MS with @jbwallingford.bsky.social @edwardmarcotte.bsky.social Ophelia Papoulas, Chanjae Lee, David Taylor, and @builab.bsky.social www.cell.com/developmenta...

ChimeraX 1.9 has been released and is available now on our website

Thanks. Warp on Linux is awesome. This is my Twitter import so it is very old tweet

My last post on Twitter before.

Proudly present our new preprint on my first post here. We performed SPA on the ciliary tip and identify 7 proteins. Among them, we found the conserved protein SPEF1 at the microtubule seam. In vitro assays show that SPEF1 binds to the seam and stablizes microtubules.
www.biorxiv.org/content/10.1...

The Bui lab Twitter will stop and we are migrating to Mastodon. Anyway, the last post is to thank the @Beck_Laboratory and @jankosinski @aobarska for the new wall art decoration. NPC rules :

Our paper on the T- and N-pilus structure with the @ChristianBaron and @NatalieZeytuni labs is finally online. Great work from Amro Jaafar and @TheRealCSBlack and others.

authors.elsevier.com/a/1gElW3SNvc2%…

Taking forever but finally here. We identified close to 40 MIPs in Tetrahymena doublet. Specifically, the MIPs at the outer junction (CFAP77, OJ2 & OJ3). @dynein_awk @TheRealCSBlack @ahmad__khalifa

Just update the subtomo2Chimera withto visualize subtomo from Dynamo. Also, small fixes for relionsubtomo2ChimeraX. now you can also using stl file for visualization for more efficient memory #TeamTomo

github.com/builab/subtomo… dynamotable2ChimeraX.py

#TeamTomo Which free software is good for segmentation of cryo-tomograms? Solutions for just 1 tomogram & many tomograms are welcome.

Presenting the cryo-EM structures of T4SS T-pilus and N-pilus in a fruitful collaboration with @ChristianBaron @NatalieZeytuni @TheRealCSBlack and the twitterless Jaafar Amro. My first ever structures/publication on a bacterial protein

biorxiv.org/content/10.110…

Our lab's first MD preprint, led by my postdoc @dynein_awk . In this preprint, we examined the regulatory mechanism of post-translational modification on dynein-2 stochastic stepping behaviours.

biorxiv.org/cgi/content/sh…

My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module.

github.com/builab/subtomo…

Anybody has a script to visualize structures after subtomgram averaging from Relion or Dynamo to Chimera/ChimeraX in the context of each tomogram? #TeamTomo

#TeamTomo What is the most user-friendly template matching software for cryo-ET with samples with rather low complexity? Can't do Warp now without Windows machine.

Our paper on the structure of the outer dynein arm on the doublet of Tetrahymena is finally online.

embopress.org/doi/full/10.15…