Adam Glaser

For really big ExM tissues we made a teflon "drumhead" for one of our open-top systems that was inspired by the FEP flexvat bases for 3D printers. At high mag vibration could definitely be an issue though...

High Resolution, High-speed, 105 MP & 100 fps Global Shutter Image Sensor | Products & Solutions | Sony Semiconductor Solutions Group Sony Semiconductor Solutions Group develops device business which includes Micro display, LSIs, and Semiconductor Laser, in focusing on Image Sensor.

New 105 MP Sony IMX927 sensor (global shutter) capable of streaming data at 100 fps... 10.5 gigavoxels/sec.

www.sony-semicon.com/en/products/...

Latest imaging data from our completely re-designed ExA-SPIM system.

Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.

That is a good data point to know. We hypothesize (no data yet) that aberrations will reduce quickly with expansion factor (RI gradients proportional to density, density gradients will reduce with expansion factor ^3). Whereas the imaging path length only increases linearly with expansion factor.

ExA-SPIM2 will provide 35 mm WD with 0.5 NA.

I think there are definitely applications where higher NA is needed. If we designed a 3rd variant, I think aiming for ~0.8 - 0.85 NA (which allows for symmetric 90 deg objectives) with 20 mm WD would be very valuable.

"Expansion-assisted" is all about balancing light collection (NA^2) with practicality of engineering lenses with both high NA and long WD. We are collecting data now to quantify this, but we also believe aberrations in ExM will scale with NA^3.

So IMO go as low in NA as specimen brightness allows!

First images from world’s largest digital camera leave astronomers in awe The Rubin Observatory in Chile will map the entire southern sky every three to four nights.

First 3200 MP images from the LSST at the Vera C. Rubin Observatory!

www.nature.com/articles/d41...

A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging Recent advances in high throughput optical microscopy have achieved whole-organism scale imaging at diffraction-limited resolutions. Current microscopes, however, require making compromises between ac...

High-throughput line scanning confocal microscopy
www.biorxiv.org/content/10.1...

None of the above captures the long WD (35 mm). Perhaps we should introduce a volumetric SBP... that divides the usable WD by either the Rayleigh axial resolution, or an isotropic resolution assumption that divides by the lateral resolution -> would be very high for this lens.

Thanks for resolving the numbers.

Agreed, this was the primary motivation for ExA-SPIM in the first place. More pixel parallelization with a larger SBP, either in 2D (global shutter) or even along 1D for rolling shutter (i.e. for ASLM). Also removes some scanning bottlenecks with a low FPS sensor.

That is right, I used the equation from doi: 10.1117/1.ap.3.4.044001. The required number of samples to fully resolve the signal - also their equation uses 0.5 instead of 0.61. Lens has more information than any current standard camera sensor but potentially could be fully used in the near future.

Totally agree with you 😆 space-bandwidth product > etendue (!)

For 420 - 850 nm light the SBP is 175 - 825 megapixels. Around 500 megapixels at 500 nm.

We are excited to share our development of a new 10X lens with NA=0.50 over a 7.2 mm FOV and a 35 mm working distance (water), fully corrected from 400-850 nm. This lens will power our upcoming ExA-SPIM "2" and is available for dissemination - please get in touch if interested!

Looking forward to the @mesospim.bsky.social #lightsheet #Microscopy anniversary symposium in October with @preibischs.bsky.social @adamkglaser.bsky.social @christellelangevin.bsky.social & many more! And to the next mesoSPIM decade! 🔬 Thanks @nvladimus.bsky.social & Co for organizing it!

GitHub - HarrisonKramer/optiland: Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. - HarrisonKramer/optiland

To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...

Imaging 3D cell cultures with optical microscopy - Nature Methods This Review discusses current 2D and 3D microscopy methods for imaging three-dimensional cell cultures and emerging strategies to address key challenges.

New review out on imaging 3D cell cultures. Congratulations to Huai-Ching and @jonliu123 on leading this excellent new review! www.nature.com/articles/s41...

Curved light sheet microscopy for centimetre-scale cleared tissue imaging - Nature Photonics Light sheet microscopy with curved light sheets enables tiling-free imaging of an entire intact cleared mouse brain with lateral and axial spatial resolutions of 1.0 μm and 2.5 μm, respectively, in le...

Curved light-sheet microscopy
www.nature.com/articles/s41...

Thermal Lensing Effects in Two-Photon Light-Sheet Microscopy In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging perf...

Thermal Lensing Effects in Light-Sheet Microscopy
arxiv.org/abs/2504.02969

Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescen...

Sound-sheet microscopy - in this study, we introduce nonlinear sound-sheet microscopy (NSSM), a method to image targeted biological functions across cubic centimeters of opaque living tissue.

www.science.org/doi/10.1126/...

New SPIM system underway!

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue Light sheet microscopy is the ideal technique for multiscale imaging of large and cleared tissues, and it is desirable to achieve the highest possible isotropic resolution across the entire sample. Ho...

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue

www.biorxiv.org/content/10.1...

It’s across the board. All grants from all agencies, future ones on pause and current ones also on pause :/

Can maybe estimate based on the 35 mm full frame sensor size (36x24 mm). So probably around 1.46 micron pixels? The max rate is 3280 megapixels/sec 🤯

Canon develops CMOS sensor with 410 megapixels, the largest number of pixels ever achieved in a 35 mm full-frame sensor | Canon Global TOKYO, January 22, 2025—Canon Inc. announced today that it has developed a CMOS sensor with 410 megapixels, which is the largest number1 of pixels ever achieved in a 35 mm full-frame sensor.

New 410 MP camera sensor coming in 2025 from Canon global.canon/en/news/2025...