Amir Rahmani astormic - Bluesky Statics

When I excite with 405 nm and collect in the 475/50 channel, I basically see nothing, no membrane, no puncta. But when I excite with 488 nm, I clearly see those lysosome-like puncta, and they overlap with LysoTracker Orange. This to me doesn't make sense as Laurdan shouldn't be excited at 488!

26.02.2026 09:52 — 👍 0 🔁 0 💬 0 📌 0

Thanks for sharing! I actually know that one, and yeah, I’m not surprised that Laurdan is colocalising with LysoTracker due to internalisation of Laurdan and accumulation in endo/lysosomal compartments.

What I’m still confused about is the excitation/emission behaviour.

26.02.2026 09:49 — 👍 0 🔁 0 💬 1 📌 0

Has anyone here worked with Laurdan in mammalian cells? I recently labelled some cells and see no signal with 405 nm excitation, but I do see lysosome-like structures when exciting at 488 nm. I also co-stained with LysoTracker and most of the signal overlaps. Any thoughts?

25.02.2026 22:13 — 👍 0 🔁 1 💬 1 📌 0

The #MSCA results are out and the overall success rate is 9.6%. My friend with 96.8 score is in the reserve list! Interesting!

09.02.2026 14:41 — 👍 1 🔁 0 💬 0 📌 0

Which CAD software has AI tools? I mainly use OnShape and haven’t seen any AI tools to pop up!

18.01.2026 22:30 — 👍 1 🔁 0 💬 0 📌 0

0.28

16.01.2026 13:31 — 👍 2 🔁 0 💬 0 📌 0

The one you have is UPlanSApo but they have a new version UPlanXApo. It’s very good!

15.01.2026 20:47 — 👍 1 🔁 0 💬 2 📌 0

You’ve got to get the new 60X/1.20 W.

14.01.2026 22:02 — 👍 1 🔁 0 💬 1 📌 0

Haven’t taken a flight in 14 months ✈️
Won’t take one until April either, which’ll make it 17.
Just a reminder that travel choices do add up for CO₂. One person skipping a flight doesn’t ground a plane, but lower demand over time does mean fewer routes and frequencies.

04.01.2026 09:12 — 👍 3 🔁 0 💬 0 📌 0

Crafting New Year (super-)resolutions at cold for 2026

Happy New Year everyone 🎉

01.01.2026 00:33 — 👍 2 🔁 0 💬 0 📌 0

Winter scene featuring a punt gliding across the River Cam, near a snow-covered arch bridge, accompanied by a 'Merry Christmas from the University of Cambridge' greeting.

Merry Christmas to all our students, staff, alumni and friends around the world 🎄

📸 Lloyd Mann

25.12.2025 08:00 — 👍 27 🔁 4 💬 0 📌 0

Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!

Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.

www.biorxiv.org/content/10.6...

Happy holidays ✨🎄

24.12.2025 07:19 — 👍 20 🔁 6 💬 1 📌 0

Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social

24.12.2025 13:16 — 👍 17 🔁 3 💬 0 📌 0

Nice collection of cameras!

23.12.2025 23:06 — 👍 0 🔁 0 💬 0 📌 0

✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

22.12.2025 10:45 — 👍 76 🔁 19 💬 3 📌 2

I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.

20.12.2025 02:06 — 👍 2 🔁 0 💬 0 📌 0

Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution <p>Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging <italic>in vivo</italic>. Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system. This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.</p>

This is one of the examples of designing a objective lens+tube lens and I was always wondering why they don't include the scan lens in the combo design.

www.oejournal.org/oea/article/...

19.12.2025 18:11 — 👍 1 🔁 0 💬 1 📌 0

I’d say there is no obvious reason for adding telecentricity! It would make the lens bulkier (i guess) but wouldn’t change the image quality that much!

18.12.2025 20:11 — 👍 0 🔁 0 💬 0 📌 0

Great stuffs!

18.12.2025 19:16 — 👍 1 🔁 0 💬 0 📌 0

Tracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff:

Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...

18.12.2025 16:16 — 👍 47 🔁 17 💬 1 📌 0

Cool stuff! Congrats!

15.12.2025 11:29 — 👍 0 🔁 0 💬 1 📌 0

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos.
www.nature.com/articles/s42...

15.12.2025 10:23 — 👍 36 🔁 13 💬 6 📌 2

Interesting! So one can say the GSG's role is purely translational?

13.12.2025 16:23 — 👍 1 🔁 0 💬 1 📌 0