Amir Rahmani

But does it have to be H-1B visa?

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

Thank you! 🤓

Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.

doi.org/10.1101/2025...

I’ve never had that privilege!

I really need a keyboard blocker like this!

Huge thanks to an incredible team who made this possible 🙌 especially Aleks for his great supervision and support.

Single-molecule flow cytometry Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...

We believe smFC brings single-molecule sensitivity to scalable cell analysis.
Excited to see how this impacts biology & medicine!
Read the full story here 👇
www.biorxiv.org/content/10.1...

We applied smFC to triple-negative breast cancer (TNBC) cells:
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.

With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail

smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.

Conventional flow cytometry is powerful but has a blind spot:
❌ It can’t detect proteins expressed at very low levels (<100–1000 molecules/cell).
That means many weak but biologically crucial signals are invisible.

Single-molecule flow cytometry Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...

👀 What if your flow cytometer could see the invisible?

Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

I was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?

Image quality metrics fail to accurately represent biological information in fluorescence microscopy Image processing methods offer the potential to improve the quality of fluorescence microscopy data, allowing for image acquisition at lower, less phototoxic illumination doses. The training and evalu...

Hello! Very excited to share our latest preprint, which is great news for us but terrible news for any diehard fans of PSNR and SSIM as image quality metrics in microscopy... (1/5)
www.biorxiv.org/content/10.1...

That makes perfect sense!

Excellent work Johannes!

Would SST work for long docking strands (70–80 nt, like in DNA-PAINT SPT)? I believe ROS-induced damage is the primary cause of degradation in longer strands too, but they may be more vulnerable.

Also, does sodium sulfite affect buffer viscosity or refractive index?

A role for class I p21-activated kinases in the regulation of the excitability of the actin cytoskeleton Highlighted Article: Inhibition of class I p21-activated kinase activity leads to the constitutitive production of PI(3,4,5)P3 at the plasma membrane and a dysregulation of the actin cytoskeleton.

Feedback mechanisms involving p21-activated kinase enzymes underlie excitability of a cell's actin scaffold.

journals.biologists.com/jcs/article/...

I can't imagine there is any other way!

The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...

comparison table for v2 and v3

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

📽️ G&D Tapes 📽️

G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social

Read the full story here:
➡️ tinyurl.com/gd352899

Excellent choice of photo to begin the talk!

Speaker: Dr Meng Lu from Peking University

Check out our latest preprint, which is the latest production from our long-lasting collaboration with Andrey Klymchenko

> functionnalizing NPs with humanized antibodies (i.e. Trastuzumab).

Work performed by @vmittelheisser.bsky.social & O.Lefebvre in my lab.

www.biorxiv.org/content/10.1...

Model-free photon analysis of diffusion-based single-molecule FRET experiments - Nature Communications The authors show how to compute the autocorrelation function of FRET efficiencies and obtain dynamic information from as little as a few thousand molecules.

They showed how the conformational dynamics of single molecules can be retrieved from FRET correlation functions in diffusion-based smFRET experiments.

www.nature.com/articles/s41...

Slanted light-sheet array microscopy for large volume imaging at rates exceeding 100 Hz

arxiv.org/pdf/2506.13664