Pranav NM Shah

Neat!

Nice one Flo @schurlab.bsky.social !

Our aim was to create a kit to produce in-house 1.2 holey carbon cryoEM grids, in three simple steps, at less than half of the prize, and without due times. I think we are there. Academic teams that want to know more, receive training or order a kit, please contact grids@puxano.com.

Confidence-guided cryo-EM map optimisation with LocScale-2.0 www.biorxiv.org/content/10.1101/2025.09.11.674726v1 #cryoEM

We finally wrote up the paper for LocScale-2.0.

edu.nl/pvhxv

Please try it out. LocScale is evolving & we are actively building in new features and improvements. Your feedback will help us shape the next steps.

Code: cryotud.github.io/locscale/

Work by @alok-bharadwaj.bsky.social and Reinier.

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

Ben Vollmer and Kay Grunewald & team @cssbhamburg.bsky.social and the beautiful structure along important insights for herpesvirus entry and membrane fusion.

3D-Animation of the nanobody (light yellow/semi-transparent) inhibiting HSV-2 glycoprotein B. The different domains of gB are color-coded. Three domains are held together by the footprint of the nanobody. IMAGE: Benjamin Vollmer / Centre for Structural Systems Biology

Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de

t1p.de/cxt7a

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

One week left to apply for a fully funded PhD position in my group! Please repost. jobrxiv.org/job/max-plan...

The structure of the mammalian bornavirus polymerase complex - Nature Communications Borna disease virus 1 replicates and transcribes its negative sense RNA genome in the nucleus of infected cells. Here, the authors present the cryoEM structures of the large polymerase protein in comp...

Very pleased to have our new work out where we perform a structure/function study on the bornavirus polymerase complex with fantastic coauthors. Have a read www.nature.com/articles/s41... @warwicklifesci.bsky.social @loiccarrique.bsky.social @franziskaguenl.bsky.social

GitHub - shahpnmlab/tomcat: Tomography Catalogue Tool Tomography Catalogue Tool. Contribute to shahpnmlab/tomcat development by creating an account on GitHub.

New Title Alert: Tomcat- a web-based tool designed for cataloging and managing tomography data providing an easy way to view, annotate, and organize tomograms.

Learn more here: buff.ly/75XbvPv

#SBGrid #SBGridSoftware #StructuralBiology

Twist and Scout: Analysis and Curation of Particles in Cryo-Electron Tomography Using TANGO www.biorxiv.org/content/10.1101/2025.07.17.665296v1 #cryoEM

Latest preprint from the lab, many years in the making!

By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase.

This could have big implications for our understanding of viral replication & assembly (🧵)

www.biorxiv.org/content/10.1...

Proud to share our latest paper. doi.org/10.1016/j.cr...

Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.

Structural biology in tissue is well and truly here!

@rosfrankinst.bsky.social

Chromatic aberration (Cc) corrected cryo-EM: The structure of pseudorabies virus (PRV) using both zero-loss and energy loss electrons pubmed.ncbi.nlm.nih.gov/40499325/ #cryoEM

YouTube video by Eames Office Powers of Ten™ (1977)

powers of 10?
www.youtube.com/watch?v=0fKB...

🎀✨Recording now available ✨🎀

youtu.be/jtDunWK8g1o?...

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.

In the Bay Area? Love computational cryo-EM/ET?

Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!

Details and registration at frostbyte-cryoem.github.io

Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

⚠️ Discovery of a new mode of viral transmission:

Deltaviruses spread through a viral Trojan Horse !

These viral satellites that do not encode their own glycoproteins, are in fact physically encapsulated within their helper virus particles!

www.biorxiv.org/content/10.1...

A thread 1/14

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Congrats @ievadr.bsky.social! Good move! 😊

Would you plan to release this as a standalone tool? I think it would have big impact on sustainability as users of other tools could be compelled to get rid of their garbage mics as soon as possible.

He is starting to sound very repetitive, dont you think? I cannot hear any difference between this album and any album up until hand cannot erase…

Congrats @doryenbubeck.bsky.social !

Great stuff @tomo.science ! Quite inspiring 🥰

Same here 😆

TomoCPT is a transformer-based solution that reformulates particle detection in cryo-ET as a centroid-prediction task using Gaussian labels #CryoET #ParticlePicking #SubTomogramAveraging doi.org/10.1107/S205...