@proteincapsid.bsky.social
Postdoc at STRUBI. Using cryo-EM to see viruses better.
New Title Alert: Tomcat- a web-based tool designed for cataloging and managing tomography data providing an easy way to view, annotate, and organize tomograms.
Learn more here: buff.ly/75XbvPv
#SBGrid #SBGridSoftware #StructuralBiology
Twist and Scout: Analysis and Curation of Particles in Cryo-Electron Tomography Using TANGO www.biorxiv.org/content/10.1101/2025.07.17.665296v1 #cryoEM
19.07.2025 10:03 — 👍 3 🔁 3 💬 0 📌 0Latest preprint from the lab, many years in the making!
By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase.
This could have big implications for our understanding of viral replication & assembly (🧵)
www.biorxiv.org/content/10.1...
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Chromatic aberration (Cc) corrected cryo-EM: The structure of pseudorabies virus (PRV) using both zero-loss and energy loss electrons pubmed.ncbi.nlm.nih.gov/40499325/ #cryoEM
12.06.2025 15:48 — 👍 4 🔁 2 💬 0 📌 0
powers of 10?
www.youtube.com/watch?v=0fKB...
🎀✨Recording now available ✨🎀
youtu.be/jtDunWK8g1o?...
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
⚠️ Discovery of a new mode of viral transmission:
Deltaviruses spread through a viral Trojan Horse !
These viral satellites that do not encode their own glycoproteins, are in fact physically encapsulated within their helper virus particles!
www.biorxiv.org/content/10.1...
A thread 1/14
1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.
27.04.2025 20:49 — 👍 50 🔁 20 💬 2 📌 2Congrats @ievadr.bsky.social! Good move! 😊
23.04.2025 08:10 — 👍 1 🔁 0 💬 0 📌 0Would you plan to release this as a standalone tool? I think it would have big impact on sustainability as users of other tools could be compelled to get rid of their garbage mics as soon as possible.
10.04.2025 07:33 — 👍 2 🔁 0 💬 0 📌 0He is starting to sound very repetitive, dont you think? I cannot hear any difference between this album and any album up until hand cannot erase…
14.03.2025 19:38 — 👍 0 🔁 0 💬 1 📌 0Congrats @doryenbubeck.bsky.social !
13.03.2025 10:43 — 👍 2 🔁 0 💬 1 📌 0Great stuff @tomo.science ! Quite inspiring 🥰
08.03.2025 18:27 — 👍 2 🔁 0 💬 1 📌 0Same here 😆
08.03.2025 18:26 — 👍 0 🔁 0 💬 0 📌 0
TomoCPT is a transformer-based solution that reformulates particle detection in cryo-ET as a centroid-prediction task using Gaussian labels #CryoET #ParticlePicking #SubTomogramAveraging doi.org/10.1107/S205...
05.03.2025 14:47 — 👍 7 🔁 2 💬 1 📌 0
ProPicker: Promptable Segmentation for Particle Picking in CryogenicElectron Tomography
Figure 1
Figure 2
Figure 3
ProPicker: Promptable Segmentation for Particle Picking in CryogenicElectron Tomography [new]
Cryo-ET: 3D seg. picks particles via prompts.
Injecting arbitrary experimental data into AlphaFold has been a missing feature. With high-throughput crystallography yielding 1000s of structures/year (@covid_moonshot) & cryoET making 😍 but low-res in situ models, it's also become a necessary one for scaling up structural biology. Hence ROCKET👇!
24.02.2025 12:34 — 👍 40 🔁 10 💬 2 📌 0
I don't want to brag (mostly because I had nothing to do with it), but that's a really cool #npjViruses Review with everything you wanted to know about #giantviruses: www.nature.com/articles/s44...
#virus
Same here!
14.01.2025 19:48 — 👍 0 🔁 0 💬 0 📌 0I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for these 1829 Chlamy tomograms and annotations 🧪🧶🧬🌾🔬
29.12.2024 16:15 — 👍 20 🔁 4 💬 1 📌 0
Check out the magnificent #baculovirus structure! The unique virion architecture and structural atlas of hallmark proteins place baculo-like viruses into a separate new realm. Many insights into baculo biology and evolution. Collab with the group of Fasséli Coulibaly. www.science.org/doi/10.1126/...
23.12.2024 19:23 — 👍 107 🔁 33 💬 0 📌 4#TeamTomo #SciArt 🧪
17.12.2024 16:23 — 👍 15 🔁 2 💬 0 📌 0
1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM.
www.youtube.com/playlist?lis...
Excited to share our work describing a series of structures that allow us to propose a new model for Hantavirus genome replication by the viral L-protein. Another great experience with @loiccarrique.bsky.social @ben-nilsson-payant.bsky.social @efodor.bsky.social Jon Grimes.
doi.org/10.1371/jour...
Ah!!! Sorry Huy! 😝
05.12.2024 21:03 — 👍 0 🔁 0 💬 0 📌 0
Warp is now av on linux
github.com/warpem/warp
And it is as simple as running a conda installation
