Kiran R. Patil

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social

Another fantastic study from @lisamaierlab.bsky.social showing how non-antibiotics impact gut ecosytem. Congratulations @ghammeranne.bsky.social et al.!

@skamrad.bsky.social @kiranrpatil.bsky.social show xenobiotics impact growth of gut bacteria and their metabolic homeostasis by affecting amine metabolism ➡️ www.embopress.org/doi/full/10....

Scientists' discovery could help protect against PFAS chemicals Cambridge University scientists say a certain species of microbe in the human gut can absorb PFAS.

Scientists discover protection from PFAS chemicals

#Cambridgeshire #BBCNews

🔗: https://www.bbc.com/news/arti...

Many many thanks to @skamrad.bsky.social @guan06rui.bsky.social @indraroux.bsky.social Leon Barron lab (ICL), A. Zelezniak ab (KCL), @lisamaierlab.bsky.social @savitski-lab.bsky.social @thav-lab.bsky.social lab @tbharat-lab.bsky.social lab and apologies to those I could not locate on Blue Sky :)

p.s. the manuscript also reports a screen of 42 widespread pollutants x 14 gut bacteria for bioaccumulation and biotransformation– we followed up on PFAS because of the major risk they pose to our and environmental health.

Dr. Anna Lindell, first author of the study

Our study provides a new window into understanding the toxicokinetics of PFAS and a step towards development of scalable biotechnological solutions for PFAS removal. Thanks to fantastic team members, especially first-author Anna Lindell, and all collaborators for making this possible.

Mice colonised with human gut bacteria show increased PFAS excretion

Last but not least, we show that bioaccumulation happens within mouse gut environment and high-accumulating bacterial communities accelerate PFAS clearance through feces. Thanks to @lisamaierlab.bsky.social

We also have thermal-proteomic-profiling (TPP, @savitski-lab.bsky.social ) and metabolomic data as well as laboratory evolution experiments supporting intra-cellular localisation of these molecules and surprising high tolerance of the accumulating bacteria cells.

Artist's illustration of PFAS bioaccumulation. Peter Northrop

PFAS was thought to interact only passively with cell membranes (and thus get stuck there). Our genetic analysis shows involvement of efflux pumps and thus implies active transport. We should not repeat the mistake of assuming passive interactions - Biology is complex and full of surprises!

Cryogenic FIB-SIMS shows intracellular PFAS aggregates

This feat is achieved because these molecules aggregate in dense clumps, leaving the cellular machinery unaffected. These clumps are visible at single cell resolution with cryo-FIB-SIMS. @tbharat-lab.bsky.social

Despite their surfactant-like properties, PFAS get inside the cells and can accumulate in amounts comparable to native metabolites; without much affecting the growth!

Bacteroides have remarkable capacity to accumulate PFAS

The study is the first report of PFAS bioaccumulation by gut bacteria. Through extensive screening, we discovered bioaccumulation by a group of prevalent human gut bacteria and across a wide concentration range (as low as 0.34 nM).

Human gut bacteria bioaccumulate PFAS

Excited to share some key details on our latest research in @natmicrobiol.nature.com reporting intra-cellular accumulation of #PFAS (aka forever chemicals) by certain human gut bacteria. #microsky #mevosky #microbiomesky
www.nature.com/articles/s41...

Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances - Nature Microbiology Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances (PFAS), commonly known as forever chemicals, in intracellular aggregates. Colonization of gnotobiotic mice with bioaccumulating bac...

#NewResearch

🚨Out now!

Human gut bacteria bioaccumulate forever chemicals, in intracellular aggregates and colonization of gnotobiotic mice with these bioaccumulating bacteria increases faecal PFAS excretion. @kiranrpatil.bsky.social

#MicroSky #MicrobiomeSky 🦠

www.nature.com/articles/s41...

Great collaboration with @savitski-lab.bsky.social! Thank you for this impactful collaboration!

thank you @tbharat-lab.bsky.social lab! So cool to see the PFAS inside single cells with your technology!

Thank you @lisamaierlab.bsky.social for this great collaboration!

Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances - Nature Microbiology Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances (PFAS), commonly known as forever chemicals, in intracellular aggregates. Colonization of gnotobiotic mice with bioaccumulating bac...

Beautiful work from my friend @kiranrpatil.bsky.social . Gut bacteria can accumulate Forever chemicals and help us get rid of them! Happy we could contribute! www.nature.com/articles/s41...

Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances - Nature Microbiology Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances (PFAS), commonly known as forever chemicals, in intracellular aggregates. Colonization of gnotobiotic mice with bioaccumulating bac...

Gut bacteria to the rescue! 🦠✨
They can shield us from harmful chemicals—and the latest study from Kiran Patil's lab (@kiranrpatil.bsky.social)
uncovers how. We’re proud to have contributed to this exciting work!
#Microbiome #Detox
www.nature.com/articles/s41...

3D rendering of a bacterium with flagella cut open to show clumps of PFAS molecules inside along with the bacteria's own cellular machinery.

Researchers in the Patil Lab have discovered that certain species of gut bacteria can absorb PFAS. Boosting these species in our gut microbiome could be a new way to protect us from the harmful effects of PFAS.

Read more here: buff.ly/4FsVFsS

@kiranrpatil.bsky.social @indraroux.bsky.social

Latest study from the lab is online today (and another will be out tomorrow, stay tuned:)). We profiled effect of >1700 chemicals (drugs, pesticides,...) on secretion of amines (involved in gut-brain and gut-immune axis. ) by human gut bacteria. Brilliant work by @skamrad.bsky.social and Tara Davis

Interspecies interactions drive bacterial proteome reorganisation and emergent metabolism Species in microbial communities must respond to the presence of others to stave off resource competition or to capitalise on new resources that may become available due to additional metabolic activi...

Check out our new preprint!
We systematically map proteomic and metabolomic interactions between >100 pairs of gut bacteria, detecting metabolic interactions and functionally-related clusters of proteins.

www.biorxiv.org/content/10.1...

@kiranrpatil.bsky.social

📢 Fresh off the press and featuring new exciting experiments! 🧪
We show how glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling - happy to finally share this 😊
doi.org/10.1016/j.st...

B2B with @jesseveenvliet.bsky.social lab: doi.org/10.1016/j.st... 🤩

Sweeteners are so common in food and drinks. New work expanding our knowledge base of sweetener-bacteria interactions 👇🏽. Kudos to @sonjablasche.bsky.social @indraroux.bsky.social and others from our lab

Kudos to Ghada @kostchristian.bsky.social team and @metagenomez.bsky.social et al. for this mammoth effort! An important step forward towards understanding natural microbial communities and determinants of their fascinating diversity.

Congratulations to talented @naomiirisvdberg.bsky.social for successfully passing her PhD exam! Very proud of the latest PhD graduate from my lab and the entire team! 🙌🎉🍾

Looking for a postdoctoral fellowship? Interested in tackling real-world challenges? Then check EMBL's EIPOD-LinC fellowships.

👉 Gain transferable skills and work on self-designed projects to make a lasting impact on global issues.

✍️ More information: www.embl.org/about/info/p...

🧪 #careers

Off-purpose activity of industrial and agricultural chemicals against human gut bacteria Contamination by industrial and agricultural chemicals like pesticides are a cause of great concern due to the risk to human and environmental health. While these chemicals are often considered to hav...

IMHO, given the vast number of chemicals (and growing), and their varied chemical/biological properties, systematic in vitro studies under comparable conditions will be essential towards accelerating toxicity assessment of chemicals. My lab's 2 cents towards this: www.biorxiv.org/content/10.1...

“Manufactured Chemicals and Children’s Health..” – an excellent evidence-based case in N Eng J Med. Despite overwhelming epidemiological evidence (Note: randomised clinical trials are obviously unethical), less than 20% chemicals are tested for toxicity.

www.nejm.org/doi/full/10....