Development of AI-designed protein binders for detection and targeting of cancer cell surface proteins Artificial intelligence (AI)-based protein design opens new avenues for the rapid generation of new research tools and therapeutics, but experimental validation lags behind the computational design th...

Please also check out another exciting work on AI-based protein design, which was a fantastic team effort with Michael Hölzel, @gha14.bsky.social, Tobias Bald and many colleagues at @unibonn.bsky.social
www.biorxiv.org/content/10.1...

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We are grateful to the Hans und Ria Messer Stiftung and the cluster of excellence @immunosens.bsky.social for supporting our research at @unibonn.bsky.social and Uniklinikum Bonn

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This work was led by the phenomenal team of Alexander Hoch, Caroline Fandrey, Thomas Ebert in collaboration with Brian Coventry and David Baker at @uwproteindesign.bsky.social, Tim Kempchen at the Hölzel lab, and Sebastian Virreira Winter and Philipp Geyer from ions.bio

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Not yet in the preprint: Mass spectrometry confirmed the highly specific degradation of one target protein out of 9,677 human proteins that were quantified in cell lysates

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Fusing AI-binders to a mutant FKBP12 domain enabled drug-controlled degradation of the endogenous target protein in human macrophages

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Coupling AI-binders to EGFP enabled the antibody-free staining of an endogenous protein in primary human macrophages

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Using Transposon-Display, we identified binders for four intracellular human proteins and further improved them by saturation mutagenesis

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We developed a technology called Transposon-Display, which couples AI binders to their own DNA for pooled screening

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Transposon-Display of AI-designed binders enables manipulation of the proteome in human cells Transposon-Display is a highly scalable screening method that links proteins to their encoding DNA during expression in E. coli via a mutant transposase. Leveraging this system, we identified AI-desig...

Can AI-designed binders degrade, aggregate, stain and enrich endogenous proteins in human cells? Check out our new preprint!
www.biorxiv.org/content/10.1...

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