Dirk Remus

Happy to share our perspective on the DNA translocation mechanism by the CMG helicase.

DNA translocation by the CMG helicase: the helical inchworm model url: portlandpress.com/biochemsoctr...

Mitotic errors as triggers of cell death and inflammation - Nature Cell Biology Rizzotto et al. discuss the causes and consequences of deregulated mitosis that allow cells arrested in mitosis to escape to the next interphase, where they can initiate inflammatory signalling or und...

Excited to announce a new review article that Andreas Villunger @villungerlab.bsky.social, Dario Rizzotto (not on bsky) and I wrote. In this article, we discuss mitosis as a central cell cycle stage for integrating DNA damage, cell death and inflammatory responses. www.nature.com/articles/s41...

Hello everybody,
I am looking for an Engineer (IE) to help me start the lab at LBMC / ENS-Lyon in April.
We will investigate chromatin organization during replication in yeast.
RT appreciated!
To apply: inserm.softy.pro/offre/187863

@lbmcinlyon.bsky.social
#NewPI #3DGenome #chromatin #replication

CAD-C reveals centromere pairing and near-perfect alignment of sister chromatids Three-dimensional (3D) genome organization plays a central role in gene regulation, chromatin folding, and genome stability. Although chromosome-conformation capture (3C)-derived methods have revoluti...

Hi all!
We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids.

www.biorxiv.org/content/10.6...

#3DGenome #chromatin #Cohesin #centromere

Chromatin buffers torsional stress during transcription During eukaryotic transcription, Pol II must overcome nucleosome obstacles and, because of DNA’s helical structure, must also rotate relative to DNA, generating torsional stress. However, there is lim...

@science.org Chromatin buffers torsional stress during transcription | Science www.science.org/doi/10.1126/...

Hi all !
Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding.
We will investigate the role the 3D chromatin organization in Replication and Repair.

www.ens-lyon.fr/LBMC/equipes...


Job offers out soon!
#NewPI #3DGenome #chromatin #replication

Support a New York State Biomedical Institute for a Healthier, More Affordable Future Can you spare a minute to help this campaign?

c.org/D52b9rWDDx

93229-Postdoctoral Researcher – Laboratory of Max Wilkinson | Memorial Sloan Kettering Cancer Center

please look here for more information about a postdoc position careers.mskcc.org/vacancies/93...

Wilkinson Lab We discover and study reverse transcriptases

The Wilkinson Lab is open for science! @mskcancercenter.bsky.social

🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬

annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)

RNA polymerase II is a polar roadblock to a progressing DNA fork Nature Communications - Transcription–replication conflicts can threaten genome stability. Here, the authors show that RNA polymerase II (Pol II) is a stronger roadblock to a DNA fork in the...

Great work from Michelle Wang’s group at Cornell exploring how RNAP II and associated R-loops act as intrinsic roadblocks during mechanical DNA unzipping. These findings have mechanistic implications for transcription–replication conflict.

rdcu.be/eILVd

Congrats Maria! very well deserved... @mskcancercenter.bsky.social @mskeducation.bsky.social

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social

Just out: BRCA2 reversion mutation–independent resistance to PARP inhibition through impaired DNA prereplication complex function | PNAS www.pnas.org/doi/10.1073/... @mskcancercenter.bsky.social @kyriepappas.bsky.social #SawyersLabMSKCC

Torsion is a Dynamic Regulator of DNA Replication Stalling and Reactivation The inherent helical structure of DNA dictates that a replisome must rotate relative to DNA during replication, presenting inevitable topological challenges to replication. However, little is known ab...

If you have not already, make sure to check out two fantastic recent preprints from the Patel and Wang labs on the same topic investigated using magnetic tweezers and angular optical trapping:
doi.org/10.1101/2024... and doi.org/10.1101/2024...

Real-time imaging of rotation during synthesis by the replisome During chromosome replication, unwinding by the helicase and synthesis by the polymerases can lead to overwinding and supercoiling of DNA. The mechanical consequences of these events and resulting loc...

🚨New preprint🚨We developed a transverse flow imaging approach to directly visualize bacterial replisomes challenged by topological barriers and discovered a fast rotational mode of replisome operation. Fantastic work by thomasmretzer.bsky.social and Hamdan Lab! tinyurl.com/replisomerot...

🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome

Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells Pluripotent stem cells are susceptible to acquiring detrimental epigenetic abnormalities, posing a hurdle to their safe use in biomedical applications. Using pluripotent cells with defined genetic bac...

Our work on the regulation is out www.cell.com/stem-cell-re...! Congrats to our collaborators in the Vierbuchen and Mezey labs. It turns out that genetic variation has to be very much considered if you want to harness stem cells safely in biomedicine. #pluripotency #epigenetics #stemcells

Engineering mtDNA deletions by reconstituting end joining in human mitochondria Recent breakthroughs in the genetic manipulation of mitochondrial DNA (mtDNA) have enabled precise base substitutions and the efficient elimination of…

Combining bacterial end joining with programmable nucleases, we induced mtDNA deletions in human cells at varying heteroplasmy and examined their metabolic and cellular effects. Simple tool to apply across any cell type and engineer pathogenic mtDNA deletions. www.sciencedirect.com/science/arti...

Congrats!!

Cryo-Electron Microscopy Reveals Hidden Mechanics of DNA Replication, Sheds New Light on Cancer Target MSK researchers shed new light on G-quadruplexes, a type of secondary DNA structure that has attracted attention as a potential therapeutic target in cancer.

Shout out to Sloan Kettering @mskcancercenter.bsky.social for supporting basic science / foundational biology...

www.mskcc.org/news/cryo-el...

In our latest preprint, we uncover how CMG helicase overcomes covalent DNA-protein crosslinks. Check out our findings here:
www.biorxiv.org/content/10.1...

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

Thrilled to share first part of my post-doc work, published today in @science.org www.science.org/doi/10.1126/...
We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:

www.science.org/doi/10.1126/...

America's Greatest Workplaces for Inclusion & Diversity 2025 Newsweek is partnering with Plant-A Insights Group to honor America's Greatest Workplaces for Inclusion & Diversity 2025. These companies are committed to fostering an inclusive and diverse work envir...

MSK has been recognized as one of the national's top workplaces for inclusion and diversity by @newsweek.com magazine. 🎉

MSK earned five stars, the highest ranking, among mid to large employers in America. Learn more: www.newsweek.com/rankings/ame...

In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨

🚨 New preprint alert! 🚨 doi.org/10.1101/2025...
🧵👇 (1/)

Proud to share our latest publication in @narjournal.bsky.social! A fantastic team effort led by @marcelwerner.bsky.social and Manuel T., where we explore the chromatin dynamics at transcription-replication conflicts (TRC) sites and their impact on genome stability using a novel TRC reporter system.

Please check out our preprint where we find that mitotic transcription helps ensure ecDNA inheritance through chromosomal tethering:

www.biorxiv.org/content/10.1...

Setting up shop in advance of closing our profile on X.

Deadline to apply (Feb 10) is quickly approaching. If you are interested in learning about a postdoc opportunity at MSK, don't wait to apply.