Tenure-based Research Staff Scientist Position in Computational Structural Biology ISTA (www.ista.ac.at) is seeking a highly skilled Staff Scientist in Computational Structural Biology to support and advance the Institute’s structural biology research through the development and imp...

ISTA @istaresearch.bsky.social is hiring a tenure-track staff scientist in Computational Structural Biology, to develop tools for cryo-EM, cryo-ET, structural prediction methods and protein interaction analysis!

Please spread the word!

ista.ac.at/en/job/tenur...

I am really enjoying this one every year. Together with a great group of students we analyse subjects related to sustainability. E.g. academic travel. We even got a nice little publication out of that work: mr.copernicus.org/preprints/mr...

This year: carbon footprint of computation and AI.

Today, teaching again our sustainability course at @istaresearch.bsky.social, we discussed about the "addiction" of humanity to fossil fuels. The promise of carbon capture and storage ("we'll solve it later") is so convenient, and who cares today that it probably won't ever work ...?

Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme The specific introduction of 1H-13C or 1H-15N moieties into otherwise deuterated proteins holds great potential for high-resolution solution and magic…

Check the last paper, equally pushed forward by Darja and @iomichiamofede.bsky.social in our lab @istaresearch.bsky.social
www.sciencedirect.com/science/arti....

The "L'Oreal For Women In Science" award will help Darja complete the final piece.

#NMR #Research #LOrealAward #FWF @fwf-at.bsky.social

Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme The specific introduction of 1H-13C or 1H-15N moieties into otherwise deuterated proteins holds great potential for high-resolution solution and magic…

The specific labeling allows seeing very important functional arginines even in >100 kDa large proteins. In MAS #NMR, we probe Arg dynamics without size limits.
Special shoutout to Fede @iomichiamofede.bsky.social , a great scientist and our master of enzymes and NMR in solids and solution.

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Synthesis of Selectively 13C/2H/15N‐ Labeled Arginine to Probe Protein Conformation and Interaction by NMR Spectroscopy We offer a new labeling approach to introduce isolated 15N−13C−1H spin systems to the side chains of arginine residues. The labeling scheme delivers high-resolution NMR spectra to study large protein....

Darja combines organic-chemistry synthesis (with our great collaborator Roman Lichtenecker) of specifically isotope-labeled amino acids with protein NMR in solution and solids.
👉 Synthesis and proof of principle is here: chemistry-europe.onlinelibrary.wiley.com/doi/full/10....

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🌟 Huge congrats to Darja Rohden for winning the L’Oréal For Women in Science Award 2025! 🎓
Darja works in our group @istaresearch.bsky.social and at @univie.ac.at, funded by @fwf-at.bsky.social on chemical synthesis of amino acids and protein #nmr.
👉 Get more details in the thread.

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Join us for the Austrian Cryo-EM Symposium Nov 11–12 at @istaresearch.bsky.social!

Top speakers, cutting-edge cryo-EM, and a chance to explore Vienna & the ISTA campus.

Register now 👉 cryoem-symposium.pages.ist.ac.at #cryoEM #teamtomo

ISTA's Science Experience Center opens with a nice festival.
If you are in the Vienna area, come and enjoy.

This will accelerate #nmr studies of large proteins.

Thanks particularly to Robert Konrat and Roman Lichtenecker, Giorgia Toscano, Sonja Knödelsdorfer, Aleksandra Ptaszek @univie.ac.at , and @iomichiamofede.bsky.social and Jakob Schneider @istaresearch.bsky.social for this great collaboration.
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The specific pattern of 13C spins and deuteration allows connecting the two methyls to the backbone.
With this information and chemical-shift predictions many methyls can be assigned in a straightforward manner -- such as in our Tom70 receptor of mitochondria and a 134 kDa large dehydrogenase.

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Methyl-TROSY NMR is powerful to probe interactions, dynamics and structure in large molecule, but a significant bottleneck is the assignment of NMR signals to individual methyls.
We leverage a new isotope labeling scheme and a tailored NMR experiment to connect methyls of Leu and Val (->pic).

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A Novel HMBC-CC-HMQC NMR Strategy for Methyl Assignment Using Triple-13C-Labeled α-Ketoisovalerate Integrated with UCBShift 2.0 In this study, we describe an integrated approach for methyl group assignment comprising precursor-based selective methyl group labeling, a novel puls…

📢 New paper: a combination of a new isotope labelling with solution-NMR, allowing to assign methyl groups even in large proteins -- we've tested it with our favourite ~130 kDa enzyme and a 70 kDa receptor.
->see thread
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@istaresearch.bsky.social @univie.ac.at
www.sciencedirect.com/science/arti...

Does this comparison take into account that electric vehicles are much heavier? And a lot of the gain may be eliminated by the fact that the cars that are bought get heavier over the years?

The VISTA Science Experience Center Opening Festival offers a free, diverse program for science, art & music lovers. Several activities, incl. exhibition tours, keynotes, and panel discussions, will also be accessible with sign language interpretation.

Join us from Oct 3-5!
https://bit.ly/4mnJ3Ne

I missed this talk as my calendar was too full. Looked like a very good one. Will it be available somewhere?

Great. It usually is an inspiring conference, indeed.

I hope you had nice runs there :-)

I'd kindly propose that at least those who are directly dependent on taxes (well, that's all of us...) simply stop supporting Amazon.
Quite easy to do.

The usual argument brought forward by Amazon users is along the lines of "fast/convenient".
But how convenient is it to be robbed? They don't pay fair taxes, offer terrible working conditions, undermine local business.
What exactly is convenient there?

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Could we scientists please stop having anything to do with Amazon? Could we please stop shooting into our own foot?

Amazon massively avoids paying taxes (profit shifting, declaring losses...). We talk about hundreds of M€ (or more..) every year. This is tax money we could use for useful stuff.

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Thanks for these thoughts and for all the work of reviewing preprints and making them public. I agree, author shall be notified!

I wonder if editors also look at these reviews. That may be useful, provided no nonsensical "reviews" are posted by competitors. Not sure if that happens; I guess not.

Check out these thoughts about reviewing preprints. I agree, a change in the culture and practices would be very welcome.

Thanks for these thoughts AND for all the reviews of preprints you and your lab are doing. This is great and I really support this.
Maybe a reason why not so many people write reviews of preprints is that doing a proper useful review of a paper is a lot of work -- usually takes me a full work day.

Join us on Tuesday, September 9, at 5 PM Paris/ 11 AM Boston/8:30 PM Delhi for a talk by Dr. Vineeth Francis Thalakottoor: PyOR: A Versatile Magnetic Resonance Simulator for Learning and Teaching #NMR #NMRchat #ChemSky

@Bruker #NMR User Meetings are coming to the US. Join us either in Washington DC on Oct. 28. or in La Jolla CA on Nov. 4. 2025. You can see the agenda and register here. www.bruker.com/en/news-and-...

Check out Fede's and Darja's MAS #NMR / isotope-labeling paper.

NMR TITAN user survey This survey aims to assess the impact of NMR TITAN on biomolecular research and identify priorities for future development. The form should take approximately 5 minutes to complete. Your responses may...

I’m carrying out a short survey of NMR TITAN users - if you’ve made use of this tool in your research, or have suggestions for its future development, I’d be grateful for your input: forms.gle/MMXYwSozszhi... #nmrchat

Check out more ongoing projects about protein dynamics, methods developments and application to biophysical and biological questions and recent publications schandalab.pages.ist.ac.at and ista.ac.at
@istaresearch.bsky.social
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Read the full story here:
doi.org/10.1016/j.jm...

Congrats particularly to Darja Rohden and Fede Napoli, and thanks Roman Lichtenecker for one more joint project that shows the power of organic chemistry for specific labeling combined with NMR. @istaresearch.bsky.social @univie.ac.at
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New publication: Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme

Our selective arginine labeling yields well-resolved 1H-detected spectra in solids (and solution). We apply it to study dynamics in crystalline ubiquitin and a >130 kDa large enzyme

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