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Sebi

@sebi06.bsky.social

I am a Software Architect for AI Solutions, Python Fan, Microscopist Image Analyst and really like Skiing. Science is like magic but real.

136 Followers  |  202 Following  |  52 Posts  |  Joined: 14.01.2025  |  1.9002

Latest posts by sebi06.bsky.social on Bluesky

Ah, I see. Cool.

28.10.2025 20:39 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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napari-czitools/src/napari_czitools/_io.py at 2e587ae0a46c5128c97573c487574d3f5a7d9935 Β· sebi06/napari-czitools napari plugin to open CZI image files and their metadata - sebi06/napari-czitools

Hi @jni.codes I thought you mean what I (try) do here:

github.com/sebi06/napar...

28.10.2025 19:48 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 2    πŸ“Œ 0

I just meant that a while ago i started to read CZI images into Xarrays.

Before that I had to use custom code to label the axis sliders in Napari, but now this just works out-of-the-box thanks to labelled arrays.

27.10.2025 21:17 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
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GitHub - sebi06/napari-czitools: napari plugin to open CZI image files and their metadata napari plugin to open CZI image files and their metadata - sebi06/napari-czitools

This works already, doesn't it.

In my (experimental) github.com/sebi06/napar... I was nicely surprised to see that using Xarrays made my custom code to label axes in Napari based on dimension order useless :-)

It works now out of the box

24.10.2025 17:14 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
Client Challenge

Finally - the latest release of our pylibCZIrw supports also MacOS.

Check: pypi.org/project/pyli...

23.10.2025 17:09 β€” πŸ‘ 4    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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My favorite thingy: Direct data streaming and stage control from Java!

17.10.2025 15:04 β€” πŸ‘ 7    πŸ” 1    πŸ’¬ 0    πŸ“Œ 1

nice to see our ZEN API from @zeiss-microscopy.bsky.social in action. *.proto Files --> Java --> Pixel Stream in Fiji πŸ˜‰

If you want more info check

github.com/zeiss-micros...

github.com/sebi06/ZEN_P...

17.10.2025 19:10 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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That’s Mifobio Spirit @gdrimabio.bsky.social … fun sample thanks FrΓ©dΓ©ric Bolze

14.10.2025 09:50 β€” πŸ‘ 4    πŸ” 2    πŸ’¬ 1    πŸ“Œ 0
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GitHub - sebi06/ZEN_Python_CZI_Smart_Microscopy_Workshop: Material to showcase and test several tools and packages releated to ZEN, OAD, ZEN-API. CZI image files and python packages related to those t... Material to showcase and test several tools and packages releated to ZEN, OAD, ZEN-API. CZI image files and python packages related to those topics - sebi06/ZEN_Python_CZI_Smart_Microscopy_Workshop

Getting ready for the Smart Microscopy Workshop at the EPFL in Lausanne. Cannot wait to get started ... πŸ˜€

www.zeiss.ch/mikroskopie/...

The code for the ZEN API & Python related topics can be found here:

github.com/sebi06/ZEN_P...

13.10.2025 19:24 β€” πŸ‘ 6    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Chatkontrolle stoppen! Die EU-Kommission will Messenger-Dienste wie WhatsApp und Signal zwingen, alle privaten Nachrichten und Fotos in Echtzeit zu scannen. Angeblich zum Kinderschutz. In Wahrheit bedeutet die Chatkontrolle...

Bald kΓΆnnten ALLE Chat-Nachrichten von den BehΓΆrden durchsucht werden – selbst ohne Verdacht! Das entscheiden *heute* die Minister*innen. Sag Nein zur Chatkontrolle! Jetzt unterzeichnen ✍️ weact.campact.de/petitions/ch...

07.10.2025 21:04 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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You need to add our ZEN API interface to that site?

github.com/zeiss-micros...

01.10.2025 15:06 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

Kind off .... but by rahter by showing off out ZEN API at an AiryScan Demo.

01.10.2025 14:50 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Guess what i am just doing ...?

01.10.2025 12:39 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

Americans: tell your friends who are worried about Tylenol that there's a safe alternative available in the UK.
For a modest fee, I will send them Paracetamol, which is NHS-approved for pregnancy and for children.
You can even get it combined with decongestant to make a tasty hot lemon drink.

27.09.2025 09:07 β€” πŸ‘ 8    πŸ” 2    πŸ’¬ 2    πŸ“Œ 0

The first day is open for all (while day 2 and 3 will be hands-on with a limited number of people)

- Learn about concepts and tools available
- AI tools within ZEISS ecosystem
- Guided Acquisition without coding
- ZEN Scripting
- ZEN API - Control ZEN via Python

and more!

26.09.2025 07:47 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Hi Fans of Smart Microscopy,

Are you ready to automate your next large scale microscopy experiment and work smarter, not harder?

Join us for the Smart Microscopy workshop organized by EPFL Bio-Imaging Platform (Lausanne) and @zeiss-microscopy.bsky.social

www.zeiss.ch/mikroskopie/...

26.09.2025 07:47 β€” πŸ‘ 22    πŸ” 8    πŸ’¬ 1    πŸ“Œ 0
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I saw this in my vaccation in a cafe in France.
As a person creating AI software tools I hope that is not true but I am not so sure anymore ...

16.09.2025 06:03 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

And now the real challenge starts - the data analysis :-)

11.09.2025 17:02 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
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From #ome-ngff-validator you can open open #OME-Zarr images in a bunch of viewers, and neuroglancer's rendering of multi-channel images just got a lot nicer: ome.github.io/ome-ngff-val... - See github.com/google/neuro...

08.09.2025 08:23 β€” πŸ‘ 15    πŸ” 4    πŸ’¬ 0    πŸ“Œ 0
Abstract: Under the banner of progress, products have been uncritically adopted or
even imposed on users β€” in past centuries with tobacco and combustion engines, and in
the 21st with social media. For these collective blunders, we now regret our involvement or
apathy as scientists, and society struggles to put the genie back in the bottle. Currently, we
are similarly entangled with artificial intelligence (AI) technology. For example, software updates are rolled out seamlessly and non-consensually, Microsoft Office is bundled with chatbots, and we, our students, and our employers have had no say, as it is not
considered a valid position to reject AI technologies in our teaching and research. This
is why in June 2025, we co-authored an Open Letter calling on our employers to reverse
and rethink their stance on uncritically adopting AI technologies. In this position piece,
we expound on why universities must take their role seriously toa) counter the technology
industry’s marketing, hype, and harm; and to b) safeguard higher education, critical
thinking, expertise, academic freedom, and scientific integrity. We include pointers to
relevant work to further inform our colleagues.

Abstract: Under the banner of progress, products have been uncritically adopted or even imposed on users β€” in past centuries with tobacco and combustion engines, and in the 21st with social media. For these collective blunders, we now regret our involvement or apathy as scientists, and society struggles to put the genie back in the bottle. Currently, we are similarly entangled with artificial intelligence (AI) technology. For example, software updates are rolled out seamlessly and non-consensually, Microsoft Office is bundled with chatbots, and we, our students, and our employers have had no say, as it is not considered a valid position to reject AI technologies in our teaching and research. This is why in June 2025, we co-authored an Open Letter calling on our employers to reverse and rethink their stance on uncritically adopting AI technologies. In this position piece, we expound on why universities must take their role seriously toa) counter the technology industry’s marketing, hype, and harm; and to b) safeguard higher education, critical thinking, expertise, academic freedom, and scientific integrity. We include pointers to relevant work to further inform our colleagues.

Figure 1. A cartoon set theoretic view on various terms (see Table 1) used when discussing the superset AI
(black outline, hatched background): LLMs are in orange; ANNs are in magenta; generative models are
in blue; and finally, chatbots are in green. Where these intersect, the colours reflect that, e.g. generative adversarial network (GAN) and Boltzmann machine (BM) models are in the purple subset because they are
both generative and ANNs. In the case of proprietary closed source models, e.g. OpenAI’s ChatGPT and
Apple’s Siri, we cannot verify their implementation and so academics can only make educated guesses (cf.
Dingemanse 2025). Undefined terms used above: BERT (Devlin et al. 2019); AlexNet (Krizhevsky et al.
2017); A.L.I.C.E. (Wallace 2009); ELIZA (Weizenbaum 1966); Jabberwacky (Twist 2003); linear discriminant analysis (LDA); quadratic discriminant analysis (QDA).

Figure 1. A cartoon set theoretic view on various terms (see Table 1) used when discussing the superset AI (black outline, hatched background): LLMs are in orange; ANNs are in magenta; generative models are in blue; and finally, chatbots are in green. Where these intersect, the colours reflect that, e.g. generative adversarial network (GAN) and Boltzmann machine (BM) models are in the purple subset because they are both generative and ANNs. In the case of proprietary closed source models, e.g. OpenAI’s ChatGPT and Apple’s Siri, we cannot verify their implementation and so academics can only make educated guesses (cf. Dingemanse 2025). Undefined terms used above: BERT (Devlin et al. 2019); AlexNet (Krizhevsky et al. 2017); A.L.I.C.E. (Wallace 2009); ELIZA (Weizenbaum 1966); Jabberwacky (Twist 2003); linear discriminant analysis (LDA); quadratic discriminant analysis (QDA).

Table 1. Below some of the typical terminological disarray is untangled. Importantly, none of these terms
are orthogonal nor do they exclusively pick out the types of products we may wish to critique or proscribe.

Table 1. Below some of the typical terminological disarray is untangled. Importantly, none of these terms are orthogonal nor do they exclusively pick out the types of products we may wish to critique or proscribe.

Protecting the Ecosystem of Human Knowledge: Five Principles

Protecting the Ecosystem of Human Knowledge: Five Principles

Finally! 🀩 Our position piece: Against the Uncritical Adoption of 'AI' Technologies in Academia:
doi.org/10.5281/zeno...

We unpick the tech industry’s marketing, hype, & harm; and we argue for safeguarding higher education, critical
thinking, expertise, academic freedom, & scientific integrity.
1/n

06.09.2025 08:13 β€” πŸ‘ 3240    πŸ” 1652    πŸ’¬ 100    πŸ“Œ 285

Ah OK. Slow means it takes too much time for a single DF3 run?

03.09.2025 20:21 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

What exactly is not convincing and should be improved? And did you try the Microscopy Copilot?

02.09.2025 18:45 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 2    πŸ“Œ 0
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ez-zarr: A Python package for easy access and visualisation of OME-Zarr filesets Barbiero et al., (2025). ez-zarr: A Python package for easy access and visualisation of OME-Zarr filesets. Journal of Open Source Software, 10(109), 7882, https://doi.org/10.21105/joss.07882

We’re excited to share that ez_zarr now supports Zarr v3! πŸš€ Huge thanks to @mbstadler.bsky.social & @csoneson.bsky.social who took on the challenge of creating this open-source tool for fast, programmatic access to OME-Zarr file sets + easy plotting πŸ“Š
πŸ“„ joss.theoj.org/papers/10.2110… (1/3)

15.08.2025 13:07 β€” πŸ‘ 20    πŸ” 11    πŸ’¬ 1    πŸ“Œ 0
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Deutsche Umwelthilfe belegt: HVO100 aus AltspeiseΓΆl klimaschΓ€dlicher als fossiler Diesel β€’ Neue Studie im Auftrag der DUH: HVO100 und β€žBioβ€œ-Diesel aus AltspeiseΓΆl in der Gesamtbetrachtung von der Produktion bis zum Verbrauch noch klimaschΓ€dlicher als fossiler Dieselβ€’ Versprechen von fast ...

#HVO100 und "Bio"-Diesel aus AltspeiseΓΆl ist klimaschΓ€dlicher als fossiler Diesel – das ergibt unsere neue Studie. Besonders relevant sind dabei KlimaschΓ€den durch Verlagerungseffekte in der PalmΓΆlproduktion. www.duh.de/presse/press...

22.08.2025 09:27 β€” πŸ‘ 76    πŸ” 36    πŸ’¬ 4    πŸ“Œ 2

Another topic is that Smart Microscopy often requires dedicated (even low level) hardware control. Therefore making all those solutions "interoperable" across imaging system will be really, really, really hard ...

20.08.2025 18:29 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

I think one reason for the fragmentation is that it so much fun to create "your own smart microscopy solutions" (I know what I am talking about 😜). This is also the reason why existing commercial solutions are often not used ...

Plus the publishing issue that new is "cooler" than "re-use".

20.08.2025 18:29 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
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I finally managed to upload the first version of the napari-czitools plugin (still in alpha - expect issues...)

- open complete CZI images or subsets
- read CZI metadata

pypi.org/project/napa...

or

github.com/sebi06/napar...

20.08.2025 18:23 β€” πŸ‘ 32    πŸ” 8    πŸ’¬ 0    πŸ“Œ 0

Available Tools:

- Guided Acquisition incl. AI Segmentation
- Experiment Feedback
- Automated Photomanipulation
- ZEN Internal Scripting Interface
- ZEN API: Control ZEN "from the outside" using Python & more incl. direct access to Pixel Stream

Maybe they should be mentioned there as well 😜?

20.08.2025 18:19 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

As you might know I am big fan of smart microscopy because I worked on that topic in academia and also in several industry position (still do).

Therefore I have to point out that @zeiss-microscopy.bsky.social offers several tools that are not mentioned as "Industry Implementations".

20.08.2025 18:19 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

Are the slides available?

20.08.2025 07:05 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

@sebi06 is following 20 prominent accounts