Lucien Hinderling

Low-Cost 3D Printed Optics for Super-Resolution Multifocal Structured Illumination Microscopy https://www.biorxiv.org/content/10.1101/2025.10.09.680962v1

Un-frigging-believable. I have waited for something like this for so long!

Hi Fans of Smart Microscopy,

Are you ready to automate your next large scale microscopy experiment and work smarter, not harder?

Join us for the Smart Microscopy workshop organized by EPFL Bio-Imaging Platform (Lausanne) and @zeiss-microscopy.bsky.social

www.zeiss.ch/mikroskopie/...

... and 7 academic labs! @olivierpertz.bsky.social @toettch.bsky.social @hannah-superres.bsky.social @alfredorates.bsky.social
Your implementation is missing? Want to join our monthly meetings on smart microscopy approaches? Contact us & find out more on
smartmicroscopy.github.io

Now featuring contributions from 6 microscope brands (Inscoper, Zeiss, openUC2, Luxendo, Cytely, Nikon)
@inscoper.bsky.social @zeiss-microscopy.bsky.social @openuc2.bsky.social @healthcare.nikon.com ...

screenshots of some of the sample implementations

Screenshot of smartmicroscopy.github.io/implementations.html, showing an overview of collected implementation examples

Want to get started with smart microscopy? 🧠🔬
Check out our online repository with implementations from labs and industry -- lots of practical tips and links to sample code! smartmicroscopy.github.io/implementati...

More details in the ✨updated preprint!✨
www.biorxiv.org/content/10.1...

seems to also contain a lot of useful IO/processing/filtering functions i find myself reimplementing again and again across projects
mannlabs.github.io/scPortrait/i...

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

New release of #BigTrace plugin is out. Together with @aafkegros.bsky.social from Simone Köhler group we added a fully automatic tracing mode. + more macro functions and bug fixes github.com/ekatrukha/Bi...

🧵1/14 Preprint thread! Can we predict a cell’s fate based on its dynamics? 🔮 Our new study unveils a framework for watching development unfold in real-time, revealing how a cell's shape and movement encode info about its future fate. 🔬📄 Preprint: tinyurl.com/4shf8v4x

we found that nuclear envelope rupture triggers rapid DIAPH3 formin influx to generate a actin mesh to prevent further chromatin leakage from damaged nuclei. thanks to Pitter Huesgen @degradomics.bsky.social and @cibss.bsky.social, @chrkam.bsky.social

Fibroblast co-culture shapes Microvascular network on a chip

HUVEC/human adult lung fibroblast (5/1) in fibrin hydrogel➡️a sustained, arborized & perfusable microvasculature

Gravity-driven flow prunes the network 🤓

Void-based microvascular morphometry

bioRxiv 2025
www.biorxiv.org/content/10.1...

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

GitHub - cxli233/FriendsDontLetFriends: Friends don't let friends make certain types of data visualization - What are they and why are they bad. Friends don't let friends make certain types of data visualization - What are they and why are they bad. - GitHub - cxli233/FriendsDontLetFriends: Friends don't let friends make certain ty...

My most successful outreach effort by far is the "Friends Don't Let Friends Made Bad Graphs" repository. It has 6.8 bookmarks on GitHub. That's just people with a GitHub account, not counting those who have used it but don't have a GitHub account.

github.com/cxli233/Frie...

Figure 1. The excitation and emission peaks of G-Ca-FLITS are red-shifted relative to Tq-Ca-FLITS. (A) The excitation and emission spectra show in lighter lines the calcium-free state (10 mM EGTA) and the darker lines the calcium-bound state (39 μM free Ca2+). The Tq-Ca-FLITS is shown as a reference and was reported before (van der Linden et al., 2021). Spectra are normalized to the maximum of the calcium-free state for G-Ca-FLITS. (B) The photo shows the fluorescence from tubes of purified biosensors when excited with UV (312 nm).

We have updated our preprint "A green lifetime biosensor for calcium that remains bright over its full dynamic range" with some new data - including this photo showing the difference in color for the Turquoise and Green lifetime probe. www.biorxiv.org/content/10.1...

Join our workshop to explore remote user access experiences at our Finnish Advanced Microscopy Node and at the Multimodal Molecular Imaging (MMMI) Italian Node. Facility experts & users will share their experiences!
🗓️ September 23 at 15:00 CEST
Register 🔽
www.eurobioimaging.eu/events/user-...

Just posted (bioRxiv): A novel #mechanobiology model revealing how #immune #Tcells combine a #nucleus as a #piston, an #uropod, and #microenvironment to power #amoeboid #cellmigration in #cellconfinement via a hydraulic cell engine. #CellBiophysics #ImmuneMigration
www.biorxiv.org/content/10.1...

📣 Call for papers: "Taking the Stage: The Future of Microscopy"

The Journal has launched an open call for papers for an exciting new special issue which will feature submissions by Early Career Researchers. The issue will be guest edited by Katherine Paine, Myfanwy Adams and Colum O'Leary.

YouTube video by Diffraction Limited An Open Source Motorized XYZ Micro-Manipulator - Affordable sub µm Motion Control

An Open Source Motorized XYZ Micro-Manipulator - Affordable sub µm Motion Control
www.youtube.com/watch?v=MgQb...

Excited to share my postdoc work from the Eggeling lab in Jena (@leibnizipht.bsky.social, #FSUJena, #KTHuniversity) on bringing smart microscopy to super-resolution MINFLUX: event-triggered MINFLUX microscopy.

Are different cell types like oil and water? Over a century ago, researchers found that dissociated sponges and embryonic tissues can self-assemble into functional structures. Join me, @benswedlund.bsky.social to explore the differential adhesion hypothesis & its applications in tissue engineering!

BIG NEWS!! We will be hiring a new professor with 25 years of endowed funding at the @biozentrum.unibas.ch @unibas.ch, focused on using biophysics and structural biology to explore climate-related fundamental science topics. Come start an innovative research cluster with us! 🧪🔬🧶🧬🌾🌍

Visual of segmented human induced pluripotent stem cells with the key workshop dates: Symposium on November 10-11, Hackathon on November 12-14th in Zurich Switzerland

We're happy to announce the 2025 International OME-NGFF workshop 🎉

📅 Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025

📍 Location: Zurich, Switzerland

Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up... 

#bioimaging #openscience #OMENGFF #OMEZarr

(1/x 🧵)

Once is an exception, twice is a trend, thrice is a tradition!

Save the date for our now-traditional annual OME-NGFF extravaganza in Zurich!

Whether you’ve just heard about OME-NGFF and are curious to learn more or if you are working with it every day, there is something for everyone this year!

The actin cortex acts as a mechanical memory of morphology in confined migrating cells - Nature Physics Cells often navigate through confined spaces. Now it is shown that cells retain a mechanical memory of previous confinement events, which makes them more efficient at migrating through narrow microenv...

Excited to share our new publication in Nature Physics about the mechanical memory of morphology in confined migrating cells by @kyohalie.bsky.social 🥳

Super fun collab with @davidbrueckner.bsky.social and @gcharras.bsky.social 👏🏻

Enjoy the reading www.nature.com/articles/s41...

🧬🔬🧪 🎉 Our team at @czbiohub is thrilled to share TWO companion papers out today in @NatureMethods!
📦 Ultrack — robust, scalable nD cell tracking
🌐 inTRACKtive — a beautiful, open-source web viewer for lineage exploration
Let’s dive in! 👇
www.nature.com/articles/s41...
www.nature.com/articles/s41...

Microalgae as a Source for Stereolithography (SLA) 3D Printing Resin 3D printers are becoming common household and laboratory appliances thanks to the possibility of producing physical objects in a short time, at low cost, with low waste, and tailored to the needs of each user. One of the most widely used types of 3D printers is stereolithography (SLA) or masked stereolithography ((m)SLA), which uses light to photo cross-link a liquid resin to solid objects layer by layer. 3D printable resins consist of numerous components, with the biggest part consisting of usually methacrylates or acrylates. However, contrary to the trend of reducing plastic consumption, the methacrylates used for SLA resins are mainly petroleum derivatives. Biobased options made with soybean oil have become commercially available in the past few years. These oils are not very sustainable as soybeans have a high demand for land and water use, and there is competition for using soy as food or feed or for materials. In contrast to soybeans, microalgae have simple nutrient requirements and do not need arable land or freshwater, excluding them from competition with crops. They also have high CO2 fixation and can produce and store a high oil content of up to 75% by biomass weight, which can be used for the production of the resin. Here, we show how to produce 3D printable resins based on microalgal oil. Starting from commercial microalgal oil, we perform epoxidation and methacrylation reactions on it to obtain reactive groups that can be used for polymerization. Subsequently, we formulated an SLA resin that we 3D printed and analyzed for mechanical performance.

YO WHAT? how cool is that! by @vsaggiomo.bsky.social: "we show how to produce 3D printable resins based on [...] commercial microalgal oil, we perform epoxidation and methacrylation reactions on it to obtain reactive groups that can be used for polymerization"
pubs.acs.org/doi/full/10....

sure! we call it the "supertracker construct" -- its lifeact::miRFP + H2B::miRFP, marks both nucleus and cell outline for tracking/KTR quantification/segmentation

Collective cell motion has many forms, but rotation is the coolest of them all.

I'm @onenimesa.bsky.social , and in this short🧵, I'll highlight some instances of global tissue rotation like this one from @BauschLab

pretty to the eye (or metric) ≠ good for analysis