Lucien Hinderling

🚀 New preprint out!

We introduce SMOLM-LFM: a simple, ratiometric, light-field–based method for 6D single-molecule orientation + localization, no polarization optics, no complex PSFs.
Fast.Robust.Large DoF.
A work done at @henriqueslab.bsky.social. In colaboration with the great L. AlemanCastaneda

YouTube video by Blender Microscopy Nodes: handling large biological volumes — Blender Conference 2025

I gave a talk at Blender Conference yesterday, showing how biological volumes work, and how to visualize them in Blender 😋 youtu.be/WPajSWX730o?... #bcon25

different journal, sorry!

they sell not only sell front-cover but also back-, front-inside- and back-inside-cover 🙃

Microscopy preprints – bioimage analysis - FocalPlane Microscopy preprints – bioimage analysis - News

📢Check out our latest #preprint list on FocalPlane
This list focusses on the latest research on bioimage analysis and data management. Let us know if there are any preprints that we should add to our list and send us recommendation for the next one!
focalplane.biologists.com/2025/11/14/m...

BigVolumeBrowser: a new 3D multi volume/mesh/point clould (SMLM) data viewer Hello everyone, I’d like to share with you another 3D viewer for FIJI, BigVolumeBrowser (full documentation link). It‘s a first initial public release, so there is still space for improvements. Le...

Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2)
forum.image.sc/t/bigvolumeb...

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods 4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt

Excited to share a new preprint! Wireless devices use FM modulation to transmit multiplexed noise-resistant data. Led by @born2raisecell.bsky.social, we create a biochemical analogue of this paradigm using genetically encoded oscillators (GEOs) for single-cell FM streaming tinyurl.com/nbs8rw42 🧵

Segment Anything Playground | Meta A playground for interactive media

you can test it here with your own data: www.aidemos.meta.com/segment-anyt...

quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"

The new open source syringe pump control boards arrived. Exciting!

The first meeting was a blast and the second one promises to be even better - if you submit your abstract and join us!

Last-minute advert for the Convpaint workshop at I2K by Roman Schwob! Interactive segmentation in @napari.org. Today 2:00 PM CET. Free registration:
www.i2kconference.org/workshops

🚨New paper! 🚨
@jasminealqassar.bsky.social led this work on the silk glands of the pantry moth.

These two long tubes inside the caterpillar continuously make a ton of silk
How does this special organ work?

www.cell.com/iscience/ful...
@cp-iscience.bsky.social

🧵THREAD🧵

Hackathon participants group picture

We had an amazing OME-NGFF hackathon last week, Wednesday to Friday, following the inspiring OME-NGFF symposium. What a fantastic week! It’s hard to describe how motivating it is to be surrounded by so many talented, curious & friendly people who care deeply about open, FAIR bioimaging data.
1/14 🧵

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇

www.biorxiv.org/content/10.1...

Dish soap. Seriously. 😆

Felix Zhou's u-Segment3D is out:

www.nature.com/articles/s41...

It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.

It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.

Check out this sneaky lamin A knockdown cell (pink dot) swimming against the tide (migration into gap) & slithering between its sisters.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.

Hi Fans of Feedback Microscopy,

what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?

Btw, it works as well in Fiji&ImageJ :-)

Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.

Intestinal villus cells extrude living, following a calcium pulse. Unlike from extruding apoptotic cells, Ca2+ messaging stops at the first neighbors! #FluorescenceFriday

Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...

Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social

E-cadherin mechanotransduction activates EGFR-ERK signaling in epithelial monolayers by inducing ADAM-mediated ligand shedding Epithelial stretching promotes the release of EGF receptor ligands that stimulate ERK activation.

A bit delayed, but excited to still share our latest paper, showing that intercellular forces transduced by E-cadherin activate EGFR-ERK signaling in epithelia by inducing EGFR ligand shedding! Mechanical and biochemical signals can act together within a single, linear cascade! tinyurl.com/mr9mj9j2

We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!

www.sarstedt.com/en/products/...

We are planning the build of a light sheet setup and are considering a Powell lens to generate the sheet.
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!

What a gem from @dudinlab.bsky.social @gautamdey.bsky.social @centriolelab.bsky.social in Cell! Expansion microscopy atlas of >200 eukaryotes comparing cytoskeletal architectures revealing structures not seen before. Stunning visualisation! Exactly the kind of transformative cell biology we need.

🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky

www.cell.com/cell/fulltex...

GitHub - openMLA/photon-ultra-controller: Raspberry pi hat for the Anycubic Photon Ultra UV projector. Implements parallel video for the DLPC1438. Raspberry pi hat for the Anycubic Photon Ultra UV projector. Implements parallel video for the DLPC1438. - openMLA/photon-ultra-controller

Also see this repo: github.com/openMLA/phot...! Haven't tested any of the open source DMDs specifically built for microscopes yet, but there shouldn't be any reason why they wouldn't work. Some projects are linked in the discussion of the paper... Would love to get my hands on one!

Working on it!! You extract all the hardware (DMD+UV LED) from a consumer 3D printer for $300, while Mightex is 30k... Early tests below, showing how we can increase the printing resolution by changing the optics. Using an inverted vintage canon camera lens as objective 😅

microfabricated stamps, fresh off the printer :)