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Léa Swistak

@leaswistak.bsky.social

🇪🇺 | Cellular microbiology | Cryo-ET | EIPOD-LinC postdoctoral fellow Mahamid lab @EMBL | Before PhD in the Enninga lab @InstitutPasteur

97 Followers  |  106 Following  |  3 Posts  |  Joined: 16.01.2025  |  1.498

Latest posts by leaswistak.bsky.social on Bluesky

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New paper from my colleague Soheil in the Mahamid and @jonasries.bsky.social groups looking at factors influencing laser devitrification on frozen #cryoem samples. With a far-red fluorophore, all-gold support grids will be especially good for correlative cryo-SMLM and EM!

doi.org/10.1016/j.js...

19.07.2025 02:32 — 👍 12    🔁 3    💬 0    📌 0
Shigella, the first international meeting

Only 328 days to go! We're very busy behind the scenes getting thigns ready for the meeting! In the meantime you can add your email address for updates here: coming-soon.embo.org/w26-04

27.05.2025 15:22 — 👍 7    🔁 9    💬 0    📌 4

Diving “Google earth mode 🌍” in a Shigella infected cell 🔬🔎
Together with @m-albert.bsky.social

26.05.2025 09:46 — 👍 16    🔁 5    💬 0    📌 0

Nicely spotted!

08.05.2025 13:12 — 👍 0    🔁 0    💬 0    📌 0
Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.

Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.

How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z

02.05.2025 17:00 — 👍 29    🔁 10    💬 0    📌 0

Great event! Super experience as a speaker/trainer. Looking forward for the next edition 😄

17.02.2025 23:57 — 👍 4    🔁 0    💬 0    📌 0

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