T3SS

Bacterial effector OspB hijacks apoptosis through peptide-bond recombination of BH3 domain proteins Shao et al. show that the Shigella effector OspB blocks apoptosis by enzymatically remodeling host BCL-2 proteins through peptide-bond recombination. This reveals a unique pathogen strategy to rewire ...

Bacterial effector OspB hijacks apoptosis through peptide-bond recombination of BH3 domain proteins: Cell Host & Microbe www.cell.com/cell-host-mi...

IscR-mediated sensing of iron-sulfur cluster demand coordinates virulence gene expression in Yersinia pseudotuberculosis The type III secretion system (T3SS) is a needle-like appendage that translocates effector proteins into host cells to disrupt host defenses. Strict control of T3SS expression is critical for facultat...

IscR-mediated sensing of iron-sulfur cluster demand coordinates virulence gene expression in Yersinia pseudotuberculosis

www.biorxiv.org/content/10.1...

Celebrated every 15 October, Global Hand Washing Day aims to raise awareness of the importance of hand hygiene in curbing the transmission of certain diseases and the spread of antimicrobial resistance.

New in JB: Déraspe, Roy et al. perform a comparative genomics study of the PA7-clade of P. aeruginosa - now recognized as a distinct species, Pseudomonas paraeruginosa. This analysis revealed 2 sub-clades, the lack of T3SS & multiresistance.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages - Communications Biology Edwardsiella piscicida T3SS effector EseJ inhibits PANoptosis (pyroptosis, apoptosis and necroptosis) in murine macrophages by activating TAK1 and disrupting the formation of NLRP3-caspase-8-RIPK1 PANoptosome, thereby enhancing bacterial virulence.

T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages
www.nature.com/articles/s42...

Injectisome assembly primes Pseudomonas aeruginosa for Type III secretion Many Gram-negative pathogens, including Pseudomonas aeruginosa , use a Type III Secretion System (T3SS) to intoxicate eukaryotic cells. The T3SS is an important virulence factor linked to increased mo...

Injectisome assembly primes Pseudomonas aeruginosa for Type III secretion
www.biorxiv.org/content/10.1...

First, first-author publication from undergrad Tabitha Cowell is live!

She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!

I'll make an explainer sometime this week.

📢A new effector-triggered immunity role for GBP1! No place for pathogens to hide now that GBP1 can detect extracellular pathogens that hijack the actin cytoskeleton. Surprise*suprise* GBP1 does this independently of LPS! 🧵
biorxiv.org/content/10.1...

Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome | mBio Bordetella bronchiseptica and Bordetella pertussis are two closely related respiratory pathogens that employ their T3SS injectisome to deliver the BteA effector into host cells. In this study, we visu...

Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome | mBio journals.asm.org/doi/10.1128/...

Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion Host mucins and synthetic glycopolypeptides block Salmonella virulence via glycan-mediated suppression of Salmonella pathogenicity island 1 (SPI-1). In this study, Wheeler et al. reveal that gastroint...

Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion: Cell Reports www.cell.com/cell-reports...

Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system | PNAS CRISPR-associated (Cas) endonucleases and their derivatives are widespread tools for the targeted genetic modification of both prokaryotic and euka...

Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system | PNAS www.pnas.org/doi/10.1073/...

Structure-Based Identification of Salvianolic Acid B as an Inhibitor Targeting Salmonella InvC ATPase Multidrug-resistant (MDR) Salmonella poses a significant global health threat. The Type III Secretion System 1 (T3SS-1) and its ATPase InvC are crucial for virulence and promising drug targets. Throug...

Structure-Based Identification of Salvianolic Acid B as an Inhibitor Targeting Salmonella InvC ATPase - Journal of Biological Chemistry www.jbc.org/article/S002...

Proximity labelling reveals VPS13C as a regulator of Salmonella-containing vacuole fission Author summary Salmonella enterica serovar Typhimurium (Salmonella) is a bacterium present in our food supply that can cause food poisoning. These bacteria can also cause severe disease in immunocompr...

Proximity labelling reveals VPS13C as a regulator of Salmonella-containing vacuole fissionhttps://dx.plos.org/10.1371/journal.ppat.1013507

Regulation of host immunity by a novel Legionella pneumophila E3 ubiquitin ligase Author summary Successful pathogens have evolved virulence factors to co-opt the host ubiquitin network to counteract immunity or to redirect host cellular events to create a niche conductive for thei...

Regulation of host immunity by a novel Legionella pneumophila E3 ubiquitin ligase dx.plos.org/10.1371/jour...

The type III secretion system translocases IpaC, SipC, and BipC are partially folded alpha helical proteins lacking in tertiary structures The Type III secretion system (T3SS) is essential in the virulence of many bacterial pathogens that cause infectious diseases in humans. The T3SS consists of an injectisome that bacteria use to inject...

The type III secretion system translocases IpaC, SipC, and BipC are partially folded alpha helical proteins lacking in tertiary structures
dx.plos.org/10.1371/jour...

Point of View: How the Global South is reshaping scholarly communication Publishing initiatives in Latin America, Africa, and Asia are promoting diamond open access, multilingualism, and non-profit, scholarly-led governance structures.

A blueprint for a fairer system! Our new article explores how the GlobalSouth is pioneering equitable, multilingual, and community-driven models of scholarly publishing. #OpenScience #SciComm Read it here, via @elife.bsky.social @elifecommunity.bsky.social 📖

elifesciences.org/articles/108...

Shigella type-III secretion system effectors counteract the induction of host inflammation and cell death | The EMBO Journal imageimageBacterial pathogens produce virulence effectors targeting the innate immune signaling of host cells, which activate alternative, “effector-triggered immunity” mechanisms in response. This study shows how Shigella flexneri uses its type-...

Shigella type-III secretion system effectors counteract the induction of host inflammation and cell death | The EMBO Journal www.embopress.org/doi/full/10....

The accessory type III secretion system effectors collectively shape intestinal inflammatory infection outcomes Injection of effectors via a type III secretion system (T3SS) is an infection strategy shared by various Gram-negative bacterial pathogens, many infecting mucosal surfaces. While individual T3SS ef...

Full article: The accessory type III secretion system effectors collectively shape intestinal inflammatory infection outcomes www.tandfonline.com/doi/full/10....

Engineering non-pathogenic bacteria for auto-transporter-driven secretion of functional interferon In recent years, various strategies have been developed to enable the oral administration of protein-based drugs (biologics) with the aim of overcoming the degradation and inactivation of these dru...

Full article: Engineering non-pathogenic bacteria for auto-transporter-driven secretion of functional interferon www.tandfonline.com/doi/full/10....

Single-cell analysis of genetically minimized Salmonella reveals effector gene cooperation in vivo - Nature Microbiology A minimal effector subset enables Salmonella Typhimurium to overcome bottlenecks regulated by the early innate immune response and establish infection within a CD62L+ monocyte niche in the spleen.

Single-cell analysis of genetically minimized Salmonella reveals effector gene cooperation in vivo

www.nature.com/articles/s41...

New preprint in the lab! How the mitochondrial ETC works during infection of human macrophages with #Legionella or #Salmonella? Spoiler: there are some similarities and also pathogen-specific differences. Work led by Francisco Garcia and Paula Martínez, with @cbuch.bsky.social at @pasteur.fr

Enteric (typhoid and paratyphoid) fever Enteric fever, caused by the human-restricted bacteria Salmonella enterica serovar Typhi (typhoid) and Salmonella enterica serovar Paratyphi A, B, and C (paratyphoid), affects persons residing in, or ...

Enteric (typhoid and paratyphoid) fever - The Lancet www.thelancet.com/journals/lan...

Commensal yeast promotes Salmonella Typhimurium virulence - Nature Commensal Candida albicans enhances the virulence and dissemination of Salmonella enterica subsp. enterica serovar Typhimurium.

Commensal yeast promotes Salmonella Typhimurium virulence

www.nature.com/articles/s41...

Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ Eislmayr et al. reveal a previously unrecognized role of macrophages in controlling the intracellular pathogen Shigella. By sensing bacteria and triggering IFN-γ production, macrophages, not neutrophi...

Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ: Cell Host & Microbe www.cell.com/cell-host-mi...

Interplay between SpaO variants shapes the architecture of the Salmonella type III secretion sorting platform Salmonella enterica utilizes a virulence-associated type III secretion system (T3SS) to inject bacterial effectors directly into host cells. Central to this machinery is the sorting platform (SP), a c...

Interplay between SpaO variants shapes the architecture of the Salmonella type III secretion sorting platform

www.biorxiv.org/content/10.1...

Combinatorial effects of tryptophan derivatives serotonin and indole on virulence modulation of enteric pathogens | mBio Pathogens sense a plethora of signals within the gut to successfully establish colonization by precise regulation of virulence gene expression within the right niche. Our study shows that it is crucia...

Combinatorial effects of tryptophan derivatives serotonin and indole on virulence modulation of enteric pathogens | mBio journals.asm.org/doi/10.1128/...

The predicted sorting platform dynamically associates with the type III secretion system from Xanthomonas euvesicatoria in response to the external pH - BMC Microbiology The Gram-negative bacterium Xanthomonas euvesicatoria (Xe) causes bacterial spot disease in pepper and tomato plants, and employs a type III secretion (T3S) system to translocate effector proteins int...

bmcmicrobiol.biomedcentral.com/articles/10....

A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly | PNAS The FliPQR complex constitutes a channel for export of the flagellar proteins involved in axial structure assembly. It also serves as a template fo...

A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly | PNAS www.pnas.org/doi/abs/10.1...

FliO is an evolutionarily conserved yet diversified core component of the bacterial flagellar type III secretion system | PNAS The bacterial flagellum is a complex nanomachine essential for motility, environmental sensing, and host colonization. While many of its core compo...

FliO is an evolutionarily conserved yet diversified core component of the bacterial flagellar type III secretion system | PNAS www.pnas.org/doi/abs/10.1...

A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly | PNAS The FliPQR complex constitutes a channel for export of the flagellar proteins involved in axial structure assembly. It also serves as a template fo...

A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly | PNAS www.pnas.org/doi/full/10....