Abhi Aggarwal

We haven’t observed any clear signs of cytotoxicity or aggregation, in contrast to what we’ve occasionally seen with jRGECO1a. We imaged at around 2 weeks in cultured cells and 4–6 weeks in vivo.

Fluorescence responses to increasing numbers of evoked AP for neurons expressing OCaMP or jRGECO1a.

3️⃣ Higher ∆F/F0 and SNR, increased photostability, and reduced photoswitching compared to most existing red and yellow GECIs

1P and 2P excitation and emission spectra of OCaMP. 1P excitation peak is 545 nm, 1P emission peak is 565 nm, and 2P excitation peak is 1050 nm.

2️⃣ Compatibility with non-tunable fiber lasers at wavelengths above 1000 nm

1P excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores.

Why did we develop yet another calcium indicator? 🤔/3
1️⃣ Multiplex across multiple brain regions with existing indicators

A sensitive orange fluorescent calcium ion indicator for imaging neural activity Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance...

#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪

A sensitive orange fluorescent calcium ion indicator for imaging neural activity

www.biorxiv.org/content/10.1...

Where can I get reagents?

Bacterial (pRSET) and mammalian (pAAV) expression vectors, including different promoters, Cre- and Flp- dependent vectors, and both the NGR and PDGFR membrane targeting sequences, are available from @addgene.bsky.social. /3

www.addgene.org/browse/artic...

🚨iGluSnFR4 is finally out!🚨🧪

We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️

www.biorxiv.org/content/10.1...

🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX

#Neuroscience

Hi Dani! Can you add me whenever you get a chance?
scholar.google.com/citations?us...

Thanks for organizing, can you add me?
scholar.google.com/citations?us...

Hi, thanks for organizing. Can you add me?
scholar.google.com/citations?us...

High-speed video showing glutamate lighting up as it’s released in synapses, visualizing the neurotransmitters of the brain with the fluorescent indicator protein iGluSnFR3.

Credit: Allen Institute, Aggarwal et al.

We’re hoping to have the preprint out within the next month or two🤞

First sky post 🦋 Sharing some vibrant iGluSnFR3 minis in cultured neurons!

I’d love to be added! 😊