Falk Schneider

I won’t buy a lens from you after seeing you put it upside down. 🙃

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy De novo protein design has emerged as a powerful strategy with the promise to create new tools. The practical performance of designed fluorophore binders, however, has remained far from meeting fluore...

Preprint drop! 🚨 Rhobin-tag your proteins and watch Saci glow at high temps! 🔥 Bright, photostable, live-cell imaging made possible with JF dyes. Huge thanks to @samjlord.bsky.social @acharlesorszag.bsky.social @mullinslab.bsky.social and Bo Huang lab at UCSF.😊

www.biorxiv.org/content/10.1...

Introducing mScarlet3-S2: a highly photostable and bright red fluorescent protein.

www.nature.com/articles/s41...

When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.

A microscope on the left, an identical one on the right except that it's glowing green. The left is captioned "Fluoresence Microscope", the right "Flueorescent microcscope"

Know the difference.

StayRose: A photostable StayGold derivative redshifted by genetic code expansion Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence engineering, addresses thi...

Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡

#ZebrafishZunday: Cytoplasmic (or ooplasmic) streaming leads to the segregation of embryo from yolk granules. Credit to @shamipourshayan.bsky.social & @heisenbergcplab.bsky.social. 🧪

🌹🔬🤩

High-speed neural imaging with multiplexed miniaturized two-photon microscopy Zhang et al. develop two types of multiplexed miniaturized two-photon microscopes (M-MINI2Ps) that increase imaging speed via beam multiplexing while preserving spatial resolution and demonstrate thei...

Head-mounted 2-photon miniscope voltage imaging!

Weijian Yang's group at UC Davis sped up 2p scanning by multiplexing to achieve 400-Hz voltage imaging of ~20 neurons, using ASAP4e

These are the first freely moving single-cell voltage recordings as far as I know.

URL: www.cell.com/cell-reports...

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇

www.biorxiv.org/content/10.1...

Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens...

-> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this?

www.fishersci.com/shop/product...

Mind-bender: The observable universe has ~10^80 atoms. But even a simple 1000×1000 black‑and‑white image has 2^{1,000,000} ≈ 10^{301,030} possibilities — and 24‑bit color explodes to ~10^{7,224,720}. The space of (small) images dwarfs the cosmos. 🔬🧪💻

🙏👏 me2

Congratulations 👏🎉
Fantastic news!
#newPI :)

Happy to share that I will be starting my independent research career as a Research Group Leader at the German Cancer Research Center (DKFZ). Starting from January 2026, my group will be based at DKFZ National Center for Tumor Diseases (NCT WERA), Würzburg, Germany.

Get into flow with zebrafish — soon your eyes will glow 🐟✨

Hi Fans of Feedback Microscopy,

what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?

Btw, it works as well in Fiji&ImageJ :-)

Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.

Multiple-Awesome-Colleague Alert! The first is Fred Heberle (not on bsky). His group made a series of 3-component vesicles (high-Tmelt PC-lipid, low-Tmelt PC-lipid and chol) and varied only the high-Tmelt lipid. Then they measured liquid-liquid phase transition temperatures (Tmix) of the membranes 🧵

A 17-Year-Old BJ Article Explored the Ground Rules of Phase Separation in Lipid Bilayer For Biophysics Week, members of the Publications Committee selected a few influential articles from Biophysical Journal to highlight as well as the people who wrote them. This is the first in the seri...

This week, Sarah and I were reflecting how all those years ago, when we were a new PhD student and a new faculty member, we had no idea that our data would be quantitatively relevant and actively used decades later by this awesome community. It’s humbling. www.biophysics.org/blog/a-17-ye...

Synthetic fluorophores for live-cell fluorescence microscopy and biosensing - Nature Chemical Biology Performing live-cell microscopy experiments with high spatial and temporal resolution requires fluorophores with highly optimized properties. This Review examines the progress in developing synthetic ...

🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩‍🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...

FPbase Fluorescence Spectra Viewer An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.

The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!

www.fpbase.org/spectra/

I'd only dabbled with napari, but needed to use it for a paper we're writing. This is a quick post about getting started: from installation to a folder full of thousands of processed images, with gotchas along the way.

quantixed.org/2025/11/04/a...

👇🏻 great opportunity for a PhD, great environment & PI!

Finally:
The MRC DTP at Warwick is open for applications.

Great program, small cohort, focus on Interdisciplinary Biomedical Research

www.findaphd.com/phds/program...

Deadline: 19-11-2025

Interested in more basic research?
Also offering a project with Munehiro Asally at the School of Life Sciences.

Probing molecular diffusion in living cells in vivo and in vitro

Apply through the same program #MIBTP
warwick.ac.uk/fac/cross_fa...

🚨 #PhD Opportunities 🚨
🧵

How does plasma membrane structure underpin tissue development?
Let’s find out!

Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.

🔬✨🐟

More info:
www.findaphd.com/phds/project...

Interested? Reach out!

Get out your calendars! The motors are out of quarantine and we plan to assemble in person on 8th May 2026 at University of Warwick Campus for a fun day on motors and the filaments they walk along. Livestream is planned too. More details on registration, travel bursaries etc. will be revealed soon.

Tracing fluorescence tracking’s past, present, and future Artificial intelligence and parallel computing could refine studies of the movement of single molecules inside cells.

Tracking has always been at the ❤️ of the scientific method, from planetary motion to single particles.

Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.

Take a look! 😊

www.aip.org/scilights/tr...

Call for Papers: Lipids and Lipidation | ACS Publications Chemistry Blog This Special Issue in _ACS Chemical Biology_ will highlight the latest advancements and perspectives in the chemical biology of lipids and lipidation. Submit your manuscript by May 31, 2026.

#ACSChemBio and #Biochemistry invite submissions to a special issue on Lipids and Lipidation guest edited by @chembiobryan.bsky.social and me! Find details in the #CallforPapers and submit your exciting findings to one of these journals by May 31, 2026. axial.acs.org/chemical-bio... #lipidtime

Very happy to be in Nashville today for #zebrafishzunday before my talk @vanderbilt.edu tomorrow, so here's a sneak preview of differential clearance of monomeric (turquoise) vs. oligomeric (red) amyloid beta from the zebrafish brain