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Kai Johnsson

@kjohnsson.bsky.social

Chemical biologist @ Max Planck Institut for Medical Research in Heidelberg

494 Followers  |  117 Following  |  6 Posts  |  Joined: 23.12.2024  |  1.7982

Latest posts by kjohnsson.bsky.social on Bluesky

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Molecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical Biology Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.

Molecular recording of cellular protein kinase activity with chemical labeling:

www.nature.com/articles/s41...

Congratulations to De-en Sun and the entire team.

10.07.2025 17:46 โ€” ๐Ÿ‘ 22    ๐Ÿ” 11    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

09.07.2025 19:58 โ€” ๐Ÿ‘ 52    ๐Ÿ” 23    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kรผhn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

03.07.2025 19:45 โ€” ๐Ÿ‘ 173    ๐Ÿ” 76    ๐Ÿ’ฌ 2    ๐Ÿ“Œ 3

Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1...
Congratulations to Yin-Hsi Lin!

16.06.2025 14:24 โ€” ๐Ÿ‘ 37    ๐Ÿ” 18    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Improving splitโ€HaloTag through computational protein engineering Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....

Improving split-HaloTag through computational protein engineering:

onlinelibrary.wiley.com/doi/10.1002/...

18.04.2025 20:17 โ€” ๐Ÿ‘ 33    ๐Ÿ” 8    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells Imaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product based fluorescent probes for actin filaments affec...

Check out our new fluorescent probe for imaging f-actin dynamics: ๐—ฆ๐—ถ๐—ฅ-๐—ซ๐—”๐—ฐ๐˜๐—ถ๐—ป: ๐—” ๐—ณ๐—น๐˜‚๐—ผ๐—ฟ๐—ฒ๐˜€๐—ฐ๐—ฒ๐—ป๐˜ ๐—ฝ๐—ฟ๐—ผ๐—ฏ๐—ฒ ๐—ณ๐—ผ๐—ฟ ๐—ถ๐—บ๐—ฎ๐—ด๐—ถ๐—ป๐—ด ๐—ฎ๐—ฐ๐˜๐—ถ๐—ป ๐—ฑ๐˜†๐—ป๐—ฎ๐—บ๐—ถ๐—ฐ๐˜€ ๐—ถ๐—ป ๐—น๐—ถ๐˜ƒ๐—ฒ ๐—ฐ๐—ฒ๐—น๐—น๐˜€ www.biorxiv.org/content/10.1...
Thank you @veselin-nasufovic.bsky.social

07.02.2025 19:42 โ€” ๐Ÿ‘ 40    ๐Ÿ” 13    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

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