Leo Steg

Ab-trapping - a peripheral staining artifact in antibody-based microscopy and genomics Antibodies (Ab) are essential for detecting specific epitopes in microscopy and genomics, but can produce artifacts leading to erroneous interpretations. Here, we characterize a novel artifact, Ab-tra...

🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...

14.04.2025 20:08 — 👍 69    🔁 41    💬 2    📌 9

I love the Brezel-in-DNA design :D Enjoy the time and dont forget to take your hat off!

03.04.2025 08:05 — 👍 1    🔁 0    💬 1    📌 0

Diagram of chromatin and nucleosomes from https://www.genome.gov/genetics-glossary/Nucleosome

The DNA in our cells is not naked but instead is compacted by being wrapped around nucleosomes assembled from histone proteins. In our current view, this produces the chromatin architecture that leaves some regions open for transcription while others are more compacted & transcriptionally silent 1/n

28.03.2025 11:03 — 👍 91    🔁 31    💬 3    📌 2

CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks - Nature Communications Cleavage Under Targets & Tagmentation is a rapidly expanding technique, but thorough evaluation and benchmarking against established ChIP-seq datasets are required. This study shows that CUT&T...

We're so thrilled to finally share with you the published version of our CUT&Tag optimization and benchmarking paper "CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks" - out in @naturecomms.bsky.social today: www.nature.com/articles/s41...

27.03.2025 11:16 — 👍 84    🔁 31    💬 4    📌 0

This is a reminder that there is still space in this starter pack if you want to join 🤩. Just send us a message!

05.12.2024 08:42 — 👍 8    🔁 3    💬 1    📌 0

Could I be added as well? :)

05.12.2024 09:24 — 👍 0    🔁 0    💬 1    📌 0

Advert detailing a postdoctoral scientist position at EMBL Rome. Contact: jamie.hackett@embl.it

Postdoc positions in epigenetics!

We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms

Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️

See👇or contact me for other options. Pls RS

02.12.2024 11:32 — 👍 82    🔁 57    💬 0    📌 10

Looks really cool!! Congrats to the whole team! Reading this in detail just replaced some items on this weeks To-Do list :)

26.11.2024 16:51 — 👍 2    🔁 0    💬 0    📌 0

Why not just make it "satanic biologists" to be more inclusive? I'm happy to follow biologists of all fields if they post like him!

13.11.2024 22:42 — 👍 4    🔁 0    💬 0    📌 0

Also not a PI, but what helped for me was signing up for Twitter (I guess bsky nowadays). If you follow the right people, you will get nice summaries of most papers. And you will learn which ones are really big for your field, because those will be discussed a lot. Then you can dive in to read.

12.11.2024 11:46 — 👍 1    🔁 0    💬 0    📌 0