Leo Steg

Big thanks to my co-author and all colleagues who supported this work!
@mariadimitriu.bsky.social

We also explore the idea of non-penetrant intergenerational epigenetic inheritance: why do some paternal exposures affect only a subset of offspring, and what mechanisms could explain this variability?

A father’s legacy: the sperm epigenome, preimplantation development, and paternal environment Paternal exposure to the environment can influence offspring phenotypes via a process known as intergenerational epigenetic inheritance. Such form of inheritance involves the sperm epigenome that i...

Our review on paternal intergenerational epigenetic inheritance and the instructive sperm epigenome is now out!

We summarize how the paternal genome instructs the earliest stages of embryonic development and how environmental exposures can reshape this process.

doi.org/10.1080/1750...

Embryonic signatures of intergenerational epigenetic inheritance across paternal environments and genetic backgrounds | The EMBO Journal imageimagePaternal environmental exposures have been linked with modulation of phenotype and disease risk in offspring via largely unclear mechanisms. This study employs in vitro fertilization and sin...

❓Can a father’s environmental exposures before conception influence their offspring?

We systematically tackled this - identifying effects of paternal age, environment and genetics on early embryos - as well as confounding influences

www.embopress.org/doi/full/10....

Ab-trapping - a peripheral staining artifact in antibody-based microscopy and genomics Antibodies (Ab) are essential for detecting specific epitopes in microscopy and genomics, but can produce artifacts leading to erroneous interpretations. Here, we characterize a novel artifact, Ab-tra...

🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...

I love the Brezel-in-DNA design :D Enjoy the time and dont forget to take your hat off!

Diagram of chromatin and nucleosomes from https://www.genome.gov/genetics-glossary/Nucleosome

The DNA in our cells is not naked but instead is compacted by being wrapped around nucleosomes assembled from histone proteins. In our current view, this produces the chromatin architecture that leaves some regions open for transcription while others are more compacted & transcriptionally silent 1/n

CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks - Nature Communications Cleavage Under Targets & Tagmentation is a rapidly expanding technique, but thorough evaluation and benchmarking against established ChIP-seq datasets are required. This study shows that CUT&T...

We're so thrilled to finally share with you the published version of our CUT&Tag optimization and benchmarking paper "CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks" - out in @naturecomms.bsky.social today: www.nature.com/articles/s41...

This is a reminder that there is still space in this starter pack if you want to join 🤩. Just send us a message!

Could I be added as well? :)

Advert detailing a postdoctoral scientist position at EMBL Rome. Contact: jamie.hackett@embl.it

Postdoc positions in epigenetics!

We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms

Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️

See👇or contact me for other options. Pls RS

Looks really cool!! Congrats to the whole team! Reading this in detail just replaced some items on this weeks To-Do list :)

Why not just make it "satanic biologists" to be more inclusive? I'm happy to follow biologists of all fields if they post like him!

Also not a PI, but what helped for me was signing up for Twitter (I guess bsky nowadays). If you follow the right people, you will get nice summaries of most papers. And you will learn which ones are really big for your field, because those will be discussed a lot. Then you can dive in to read.