Reed Stubbendieck

Revitalizing actinobacteria research: an urgent response to the antimicrobial resistance crisis - Natural Products and Bioprospecting Abstract The crisis of antimicrobial resistance (AMR) is escalating while the antibiotic pipeline remains stagnant. Our bibliometric analysis of eight decades of literature reveals a critical imbalanc...

Research into filamentous actinomycetes, the antibiotic producers, is in steep decline. Loads of natural products chemistry research worldwide but not much aimed at understanding their biology and ecology. The latter is key to unlocking their specialised metabolism

link.springer.com/article/10.1...

Agreed. Printing PP is a pain, but so worth it for lab stuff. There are also services you can upload an STL to and get a print that might be easier for one off pieces.

Audience attentively listening to speaker at the Women Microbiologist Network from Annual Conference 2025.

On International Day of Women and Girls in Science 🔬👩‍🔬
We’re launching the Women Microbiologists Network: a member-led networking and discussions event for women in microbiology by Dr. Enas Newire.
Read the news story and join:
🔗 https://microb.io/4ax5M5I

Would you mind sharing a reference or sequence? Would love to look into this more.

Colleague I've talked to says they specifically have the problem using sacB in plasmids with R6K origins. Anyone else experience this?

Does anyone have experience with counter-selectable markers for Gram-negative bacteria besides sacB? It's been working poorly in our hands.

Rothia similimucilaginosa sp. nov., isolated from the human nasal cavity Four strains of a Gram-stain-positive, coccoid, catalase-positive, non-motile bacterium were recovered from nasal lavage samples collected from children in Wisconsin during the Spring of 2008. These s...

My lab has published our first paper! We previously showed that Rothia are associated with healthy nasal microbiomes and inhibit the pathobiont Moraxella catarrhalis. We now describe the novel species Rothia similimucilaginosa. Check it out here: www.microbiologyresearch.org/content/jour...

I’m excited to share the first paper from my lab that was led largely by undergraduate students at University of Wisconsin and Washington College! #secmet #natprod
www.frontiersin.org/journals/mic...

Semester ended with two manuscripts accepted after over a year each in review! Happy Holidays all!

#secmet

Come and work with me and @ariannebabina.bsky.social on #Streptomyces evolution and antibiotic production

Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces

www.gla.ac.uk/postgraduate...

please repost

Prabhakar Raghavan realized in 2019 if you make search less efficient, then you can serve customers more ads.

That's a gorgeous frog!

Achromobacter spp.: Emerging pathogens in the cystic fibrosis lung

The first paper from PhD student Niladri Bhowmik is out today as a PLOS Pathogens Pearl: Achromobacter spp.: Emerging pathogens in the cystic fibrosis lung. Congrats Niladri!

journals.plos.org/plospathogen...

Recorded #MattersMicrobial Episode 88 with Dr. Reed Stubbendieck from Oklahoma State today. Great conversation about how microbes converse with one another during sickness and health. Fascinating! @bactereedia.bsky.social

Frontiers | Inexpensive Apparatus for High-Quality Imaging of Microbial Growth on Agar Plates The ability to capture images of results or processes is an important tool in the biologist’s tool kit. In microbiology, capturing high-quality images of mic...

We built this in my laboratory: www.frontiersin.org/journals/mic...

Ooof. No thank you!

Yeah. Standard LB. This is looking possible likely based on some preliminary tests we've done.

Thanks! Yeah. Agreed. Both are worth testing. Might be time to dump the competent cells.

We have been dealing with high background using erythromycin selection for multiple E. coli strains in my lab. We've tried multiple vendors and lots, but the problem persists. Has anyone else had this problem before? Any ideas?

A slack message from me saying "... is anyone waiting on something from me? I think I'm caught up again"

#NewPI life

In biology, I keep thinking of the bacterial strains, yeast strains, plasmids, mouse lines, worm mutants etc etc etc that will be lost to science FOREVER because the freezers and animal facilities won't be maintained

Generations of work will be lost in essentially an instant

Functional Metagenomic Selections for Phage Defense

My lab’s first preprint!

We used functional metagenomics to identify phage defenses in human and soil microbiomes. We scaled these selections while maintaining accuracy, enabling us to examine 9 habitats for defense elements against 7 phages.

www.biorxiv.org/content/10.1...

1/10

NSF downsizes summer research program for undergraduates Many participants are from groups underrepresented in science

"downsizes" is a weak way of saying "forced to cancel dozens of programs, which jeopardizes future STEM careers of talented undergrads" @science.org www.science.org/content/arti...

Some of my first year grad students in my class are asking for books to read that will get them thinking about what questions to ask and how to know what they should ask. I have a couple of recs but wondering if anyone can help. What inspired you??

GATOR-GC preprint is live! If you haven't already, check out our new tool for targeted gene cluster mining. @jcedielbecerra.bsky.social

doi.org/10.1101/2025...

Context matters: assessing the impacts of genomic background and ecology on microbial biosynthetic gene cluster evolution | mSystems Microbial secondary metabolites are compounds produced by bacteria and fungi that are not required for their replication and unconditional survival (1, 2). While they are thus not expected to be unive...

Our minireview on BGC evolution being shaped by genomic & ecological context is now live on mSystems:

journals.asm.org/doi/10.1128/...

#secmet #MEvoSky

Graduate Reductions Across Biomedical Sciences (2025)

We are crowd sourcing reductions in graduate admissions and hiring freezes across biomedical research and higher ed in response to pauses in NIH funding and EO’s. If you have information if you could add to this spreadsheet, it would be greatly appreciated!: docs.google.com/spreadsheets...

Just sitting here, trying to write a grant, crying for so many thousands who dedicated their lives to making the world better and safer, just thrown out like trash today.