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Jake Smith

@cryingem.bsky.social

PFIB-tamer, tissue lifter-outerer, stage-tilter, electron advocate, neuron fan Oxford/RFI - Grange Lab๐Ÿ”ฌ

565 Followers  |  372 Following  |  18 Posts  |  Joined: 14.11.2024  |  1.6175

Latest posts by cryingem.bsky.social on Bluesky

if you have to do MFA twice in the same "day": go to bed

30.09.2025 01:09 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
teamtomo.org Modular Python packages the cryo-ET community can depend on.

maybe teamtomo.org needs a blooper reel section @alisterburt.bsky.social? I have quite the impressive back catalogue from the early lift out days...

16.09.2025 15:49 โ€” ๐Ÿ‘ 5    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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Tiny Tubes Reveal Clues to the Evolution of Complex Life | Quanta Magazine Scientists have identified tubulin structures in primitive Asgard archea that may have been the precursor of our own cellular skeletons.

Lokiarchaea are tricky to work with in the lab: they grow very slowly, exclusively in an oxygen free environment with the presence of symbiotic bacteria. But with careful nurturing, scientists hope to witness their cell division at work.

10.09.2025 15:46 โ€” ๐Ÿ‘ 40    ๐Ÿ” 14    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 2
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repurposing library monitors

#upthearsenal

31.08.2025 15:38 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

woooooo!๐ŸŽ‰

19.08.2025 19:38 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

hot (well, cold) off the press! โ„๏ธ

well done @glynnca.bsky.social & @michaelgrange.bsky.social & fabulous co-authors ๐Ÿ‘๐Ÿผ

#teamtomo #cryoET

21.07.2025 20:55 โ€” ๐Ÿ‘ 10    ๐Ÿ” 1    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

thanks Jenny!!!๐Ÿซฐ๐Ÿผ

19.06.2025 09:24 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Delighted to see our work in press! ๐Ÿ™Œ๐Ÿ”ฌ๐Ÿง 

Thanks to @glynnca.bsky.social, @michaelgrange.bsky.social and the rest of the fabulous co-authors for all your contributions.

Excited to see where in tissue structural cell biology goes next ๐Ÿ‘€!

#liftlaughlove #teamtomo #whopper

19.06.2025 09:23 โ€” ๐Ÿ‘ 8    ๐Ÿ” 3    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Honestly, I don't think there is an inherent difference (willing to be proven otherwise).

My guess is it's largely due to the uptake in post-processing sorcery (denoising, deconvolution etc.)

28.04.2025 15:23 โ€” ๐Ÿ‘ 3    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Happy to chat over DM!

28.04.2025 12:35 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Congrats, Rasmus! Awesome stuff

28.04.2025 12:16 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

I am a ride-or-die Hydra fan. The most versatile of the whole bunch!

28.04.2025 12:13 โ€” ๐Ÿ‘ 5    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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inspiring and relatable talk from @alisterburt.bsky.social

#ccpem

25.04.2025 18:31 โ€” ๐Ÿ‘ 10    ๐Ÿ” 1    ๐Ÿ’ฌ 3    ๐Ÿ“Œ 0

note to self: don't turn out of office replies on

22.04.2025 17:27 โ€” ๐Ÿ‘ 4    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1

06.04.2025 22:47 โ€” ๐Ÿ‘ 6    ๐Ÿ” 3    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1

Check out our paper on Xenon milling of HPF samples, now online @naturecomms.bsky.social

rdcu.be/eczIb

07.03.2025 12:54 โ€” ๐Ÿ‘ 10    ๐Ÿ” 3    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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Barista: How caffeinated would you like to be?
Me: Yes

05.03.2025 11:51 โ€” ๐Ÿ‘ 2    ๐Ÿ” 1    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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Eukaryotes' closest relatives are internally simple syntrophic archaea Eukaryotes are theorized to have originated from an archaeal phylum Promethearchaeota (formerly 'Asgard' archaea)1,2. The first cultured representatives revealed valuable insight3,4 but are distantly ...

Isolated in just 3 years! (previously, 12 years). We obtained two strains from 'Hodarchaeales', proposed to be closest to our archaeal ancestor. Our analysis suggests the ancestor was an anaerobic, syntrophic, peptidotrophic, archaeon with a simple intracellular structure and possible aerotolerance.

27.02.2025 03:58 โ€” ๐Ÿ‘ 99    ๐Ÿ” 35    ๐Ÿ’ฌ 7    ๐Ÿ“Œ 3

A great opportunity! (especially to hear @glynnca.bsky.social spread the plasma gospel๐Ÿ™Œ๐Ÿป)

22.02.2025 13:54 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Congrats Charlie & co.! The use of optogenetics for in tissue CLEM here is really elegant.

22.01.2025 12:58 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

A long read from 2017 but well worth the time for any scientist who both loves their job and and often finds themselves questioning their life decisions. www.danielnettle.org.uk/wp-content/u...

08.12.2024 22:05 โ€” ๐Ÿ‘ 115    ๐Ÿ” 45    ๐Ÿ’ฌ 5    ๐Ÿ“Œ 15

We have quite a range of PhD projects this year, covering cryo-EM methods, bacteriology/bioenergetics, and infection/antibiotics/vaccines. Would really appreciate if people can RT and share with their teams!

29.11.2024 11:55 โ€” ๐Ÿ‘ 27    ๐Ÿ” 25    ๐Ÿ’ฌ 2    ๐Ÿ“Œ 2

Chefs kiss

26.11.2024 22:00 โ€” ๐Ÿ‘ 3    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Is there such thing as TEMTuesday? Maybe CryoETuesday? Myelinated axon cross section with microtubules and intermediate filaments:

26.11.2024 18:50 โ€” ๐Ÿ‘ 144    ๐Ÿ” 14    ๐Ÿ’ฌ 10    ๐Ÿ“Œ 1

it is nicer over here

15.11.2024 21:20 โ€” ๐Ÿ‘ 6    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

@cryingem is following 20 prominent accounts