Very excited to continue our amazing collaboration with @apacureanu.bsky.social at @esrf.fr - thank you @wellcometrust.bsky.social and all the collaborators at @crick.ac.uk and @esrf.fr

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Young Swiss Tomo Organizers Elda, Zanetta, Leonie and Florent

Kuppelraum, University of Bern

David Kalbermatter from Bern

Jean Daraspe from Lausanne

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋

Excited to share our preprint on cytoskeletal organization in astrocytes!
biorxiv.org/content/10.110…

Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.

Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓

1/x

The connectome of the entire male Drosophila central nervous system is out!! Acquired using FIB-SEM @ 8x8x8nm, we aligned 7.5 mio 2D images aligned into one seamless 3D volume, which was used to segment & proofread neurons and synapses. @hhmijanelia.bsky.social

www.biorxiv.org/content/10.1...

Genetically encoded FerriTag as a specific label for cryo-electron tomography Wang et al. present an optimized labeling strategy for cryo-electron tomography based on genetically encoded FerriTag. This system uses rapamycin to recruit iron-loaded ferritin to target proteins, en...

Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️

Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...

Excited to share our latest collaborative work from Erin Schuman's lab @mpibrain.bsky.social : An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution, now published in @natcomms.nature.com. Here’s a thread summary 🧵
doi.org/10.1038/s414...

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Brain Surfaces of 70 primate species

1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!

🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...

Representational drift without synaptic plasticity Neural computations support stable behavior despite relying on many dynamically changing biological processes. One such process is representational drift (RD), in which neurons' responses change over ...

When neurons change, but behavior doesn’t: Excitability changes driving representational drift

New preprint of work with Christian Machens: www.biorxiv.org/content/10.1...

In a new study, @victoriazhanqi.bsky.social and the Ponce Lab reveal that neurons across the primate visual cortex are tuned not just to objects, but to animal features—highlighting a surprising bias in how the brain encodes natural scenes. brain.harvard.edu/hbi_news/whe...

Tau filaments with the Alzheimer fold in human MAPT mutants V337M and R406W - Nature Structural & Molecular Biology Qi et al. used cryo-electron microscopy to determine the structures of tau filaments from the brains of individuals with MAPT mutants V337M and R406W, known to give rise to frontotemporal dementias. T...

Finally, the paper by @qcxy8886.bsky.social on #cryoEM of tau mutants V337M and R406W is out in NSMB (which is not the fastest journal). Tau filaments with these mutations have the same structures as in AD, which may be useful for the development of faster models.

www.nature.com/articles/s41...

Today was a memorable day.. saying goodbye to our trusty Tecnai 12 Spirit! >180,000 images from just Ian and myself...not counting the numerous MSc's PhD's and publications!

End of an era!

Thank you @thermofishersci.bsky.social

In collaboration with the Posthaus Lab, we identify ANTXR2—also the anthrax toxin receptor—as the receptor for Clostridium perfringens NetF pore forming toxin. Cryo-EM shows extensive contact between the pore and the receptor
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...

Happy about our new paper in Nature rdcu.be/eukUB
The GluA3 structure provides a framework for understanding this disease-prone glutamate receptor - novel interfaces will provide targets for modulator development

A vector calculus for neural computation in the cerebellum Null space theory predicts that neurons generate spikes not only to produce behavior but also to prevent the undesirable effect of other neurons on behavior. In this work, we show that this competitiv...

Neuronal computation in the cerebellum via a vector calculus.

Work of Mohammad Amin Fakharian, Alden Shoup, Paul Hage, and Hisham Elseweifi

www.science.org/doi/10.1126/...

Light-microscopy-based connectomic reconstruction of mammalian brain tissue - Nature A technique called LICONN (light-microscopy-based connectomics) allows mapping of brain tissue at synapse level and simultaneous measurement of molecular information, thus enabling quantification of c...

Mo and Hans's work here is truly a landmark in dense structural imaging, but the proof of the pudding is in the eating. Do yourself a favor and check out the Neuroglancer links and get an early glimpse at a technique I'm confident we'll see more and more over the next few years.

(1/6) Thrilled to share our triple-N dataset (Non-human Primate Neural Responses to Natural Scenes)! It captures thousands of high-level visual neuron responses in macaques to natural scenes using #Neuropixels.

Fascinating bridge between connectomics and system neuroscience!

Linking function and structure at scale with X-rays - and several times over. Massive congratulations to the team ‪@yuxinzhang.bsky.social‬, @carlesbosch.bsky.social, @apacureanu.bsky.social @esrf.fr, @crick.ac.uk and all our collaborators.

📢 New paper alert!

Comparative connectomics of Drosophila descending and ascending neurons

A new publication in Nature from our Drosophila Connectomics group and Jefferis group @jefferis.bsky.social @mrclmb.bsky.social

www.nature.com/articles/s41...

@cambridgebiosci.bsky.social @nature.com

Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET pubmed.ncbi.nlm.nih.gov/39829918/

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

The electron cryo-microscope adapted for use at liquid nitrogen temperatures within its custom-made black box.

A colder frontier in cryo-EM 🧪🔬

Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).

Read more: tinyurl.com/mwwcunkc

#LMBResearch

Hippocampus: Mapping out overlapping connectivity patterns Untangling the functional organisation of a brain region crucial for memory and learning helps reveal how individual differences are linked to variations in recall ability, aging and dopamine…

Hippocampus: Mapping out overlapping connectivity patterns.

Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells - Nature Communications Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that distinct ribosomes cooperate rath...

Out just now @naturecomms.bsky.social: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells:

www.nature.com/articles/s41...

Viruses: How herpes is assembled A combination of imaging techniques reveals how herpes simplex virus type 1 assembles within infected cells, highlighting the roles of essential viral proteins in viral assembly and exit.

I am pleased to share my eLife Insight related to an outstanding manuscript by Nahas et al. This manuscript discusses the assembly of the Herpes simplex virus through a combination of light microscopy and X-ray tomography. You can find my Insight here: elifesciences.org/articles/106....

Cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import, say Ya-Ting Chang, Danielle Grotjahn (@nanigrotjahn.bsky.social) @scripps.edu and colleagues: rupress.org/jcb/article/...

#ProteinHomeostasis #Organelles #StructuralBiology #CryoET

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

GitHub - alisterburt/napari-tomoslice: tomogram visualisation and annotation in napari tomogram visualisation and annotation in napari. Contribute to alisterburt/napari-tomoslice development by creating an account on GitHub.

New Title Alert: napari-tomoslice- a plugin for napari to aid in tomogram visualisation and annotation.

Learn more here: https://buff.ly/3Eq8cGP

#SBGrid #Software #StructuralBiology