(GIRISH)MALI LAB

Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...

The result of a fantastic Iwasa-Drubin lab collaboration.

@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...

Congrats to the whole team!

Add our first pre-print to your reading list?

Using AF2 and MD, we've characterised structures and conformational ensembles of human rhomboid proteases - and revealed some surprises along the way. Feel v.lucky to work alongside Bryony & @robincorey.bsky.social!

Please share: tinyurl.com/3h8r7f5y

This has now been published in print! Check it out:
www.science.org/doi/10.1126/...

Our work accurately measuring the 3D affinity between the OT-I TCR and a large number of foreign and self antigen ligands is now published at EMBO Journal.

Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens
www.embopress.org/doi/full/10....

JCS snapshots: the cilia and flagella edition - FocalPlane JCS snapshots: the cilia and flagella edition - News

We’re excited for the UK Cilia and Centrosome Network e-symposium this afternoon featuring the #JCSciliaSI!

To discover more about the research in this issue, check out these JCS snapshots, video highlights from authors about their research: focalplane.biologists.com/2025/11/24/j...

So proud of the lab for its achievements - Thank you to the Pettenkofer foundation for recognising our contributions - here’s to some more bacterial effector related fun in the future! @dunnschool.bsky.social

Thrilled to share our work on the 🔥 single-celled predator Podophrya collini, which rewires its cell morphology to hunt more efficiently. Huge thanks to our amazing team—Amy, Lauren, Omaya, Marine, Mari, and especially Scott—for making this shine! ✨

I am just sad to see an act of vampirism against a harmless Tetrahymena!

Key transcripts encoded centrin-like proteins, a frequent component of sensory/contractile structures in protists. We visualized tentacle ultrastructure by U-ExM with @dudinlab.bsky.social lab, discovering stunning tip and collar structures 🤯that add structural complexity to new tentacle formation.

I saw her at the poster session at ASCB 2024! very very impressive work and such a cool model system to study.

Excited to share our new preprint in collaboration with Ahmet Yildiz's lab. Check out how our team uncovers a novel binding footprint and motor regulation mechanism for MAP9 Congrats to Burak Cetin and @aryantaheri.bsky.social

www.biorxiv.org/content/10.1...

Thanks for the shoutout! @jcellsci.bsky.social :)

If you’re at #sfn25 you definitely don’t want to miss this nanosymposium on cilia, tomorrow from 1-4pm! Come learn about neuronal cilia, they do some pretty cool stuff!

An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, app...

Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...

Same, worst respiratory infection I have ever had, persistent cough only just recovering. Hope you are doing ok!

We're still accepting submissions for our 'Cell Biology of the Nucleus' special issue, which is being guest edited by
@abbybuch.bsky.social and Megan King (@luskinglab.bsky.social).
Submission deadline: 5 January
journals.biologists.com/jcs/pages/nu...
#nucleus #notforprofit #freetopublish

Chris is a brilliant structural biologist and mentor. Would highly recommend applying!

Great to see this work out! Congratulations to the team but especially James Stowell and Conny Wu (a real labour of love)

This work is now officially published
@natsmb.nature.com
with some fun new analyses included in the revised version. Congratulations to all the authors! @connyyu.bsky.social @rappsilber.bsky.social @lrsinn.bsky.social @fjoreilly.bsky.social www.nature.com/articles/s41...

Congrats Richard. Nice to see this work out!

Very excited to share this one! Our comprehensive structure, genetics and biophysics dissection of which flagellar dyneins and other proteins are needed for flagella to beat and drive cells swimming behaviours: www.biorxiv.org/content/10.1...

Wonderful to see this comprehensive study out in pre-print! Congratulations to the whole team.

In an eight-person rowing boat, each rower contributes to movement but also has a unique role: balancing, powering, or setting the rhythm and pace. In our latest collaborative work we asked – do the eight dynein “rowers” in #cilia and #flagella operate in the same way?

Our latest collaborative effort - with Eva Gluenz and @zephyris-science.bsky.social - to understand how the various proteins and complexes of the axoneme contribute to ciliary motility:

ICE is sending a chill through the construction industry The construction industry, where more than a quarter of workers are foreign-born, has long struggled to find enough workers. Now, industry officials say Trump's immigration crackdown is making it worse.

The construction industry, where more than a quarter of workers are foreign-born, has long struggled to find enough workers. Now, industry officials say Trump's immigration crackdown is making it worse.

Keren Hilgendorf

Girish Ram Mali

Also in Issue 20:
- Editorial from Pleasantine Mill @cilialab.bsky.social and Lotte Pedersen @lottepedersen.bsky.social.
- 'Cell Scientist to Watch' interviews with Keren Hilgendorf and Girish Ram Mali @dyneinassembly.bsky.social
journals.biologists.com/jcs/issue/13...
#JCSciliaSI

It was a great pleasure working with @cilialab.bsky.social and @seemagrewal.bsky.social plus others at @jcellsci.bsky.social on this special issue on ‘Cilia and flagella’. Lots of cool stuff on #cilia #flagella in there, enjoy!

We are thrilled to see this out in @jcellsci.bsky.social an in the special issue on Cilia. Cfap298 - which we call Kurly - is the mutant that keeps on giving us surprises! In this paper, a mutation generated by CRISPR, that deletes two aa and changes a third, specifically affects cilia motility.

Volume 138 Issue 20 | Journal of Cell Science | The Company of Biologists Journal of Cell Science publishes cutting-edge science encompassing all aspects of cell biology. It is published by The Company of Biologists, a not-for-profit organisation.

Our special issue on ‘Cilia and Flagella’ is now out! Huge thanks to all (authors + reviewers) who contributed, and to our wonderful Guest Editors (@cilialab.bsky.social + @lottepedersen.bsky.social) for pulling it together.
Happy reading!
#cilia #flagella

journals.biologists.com/jcs/issue/13...