Hasan Yardimci

📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...

Yadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...

Thanks Gideon!

£2.3m awarded to study DNA replication stress Hasan Yardimci, Senior Group Leader at the Francis Crick Institute, has received a Wellcome Discovery Award for £2.3m to study how cells faithfully duplicate their genetic material when under stress.

Congratulations to @hyardimci.bsky.social on receiving a £2.3m @wellcometrust.bsky.social Discovery Award to study how cells faithfully duplicate their genetic material when under stress. 🥳

www.crick.ac.uk/news-and-fea...

RNA transcripts regulate G-quadruplex landscapes through G-loop formation G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics are controlled remains poorly understood. Here, we report that RNA tra...

Excited our paper is out! G-quadruplex (G4) unwinding via an intricate G-loop assembly and disassembly mechanism maintains genome integrity. Pioneered by Koichi Sato with great collaborators Jing Lyu and @simonelsasser.bsky.social Check it out: www.science.org/doi/10.1126/science.adr0493 🧵(1/4)

New from our lab @crick.ac.uk, in collaboration with the Enchev and Bukau labs.
By studying a protein that is difficult to fold, we discover fascinating new mechanisms by which the ribosome supports protein biogenesis.
www.biorxiv.org/content/10.1...

Postdoctoral Research Associate in the Blackford Group A position as Postdoctoral Research Associate is available at the Department of Cellular and Molecular Medicine, in the group headed by Dr. Andrew Blackford. Th

We’re #hiring, please share! A fully funded #postdoc position is available in my lab to work on DNA damage response mechanisms.

Please get in touch with your CV if you are interested; closing date: 27th June 2025.

More details here: candidate.hr-manager.net/ApplicationI...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established.
www.science.org/doi/10.1126/...

Application deadline is this Friday!

3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴󠁧󠁢󠁳󠁣󠁴󠁿

www.biochemistry.org/events-and-t...

📢 For all DNA fiber enthusiasts! 🟥🟩 We are thrilled to announce the release of our long-awaited AI-based workflow for the automated and quantitative analysis of DNA fibers. It's an exciting step forward—check it out on bioRxiv! lnkd.in/dQWUPtsP

Science under siege: protecting scientific progress in turbulent times As Editors-in-Chief of The Company of Biologists' journals, we have been watching with growing concern the policy changes in the United States of America (USA) and the challenges that these are creati...

Science under siege: protecting scientific progress in turbulent times

journals.biologists.com/dev/article/...

Resolution of collapsed forks is separate from completion of DNA synthesis Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...

🧬⛓️‍ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

In our latest preprint, we uncover how CMG helicase overcomes covalent DNA-protein crosslinks. Check out our findings here:
www.biorxiv.org/content/10.1...

A new PhD position is available in my lab to explore DNA replication mechanisms using single-molecule approaches. Apply by 19th of March:

www.crick.ac.uk/careers-stud...

SAVE THE DATE! The next EMBO | EMBL Symposium on 'DNA replication and genome maintenance: from basic biology to disease' will be held October 20-23, 2026.
 
Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).

www.embl.org/about/info/c...

Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions Schmid and Walter train a classifier that discerns functionally relevant structure predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer, and they use this co...

Very excited to share that my thesis work is out in Molecular Cell! We trained a Structure and Omics informed Classifier (SPOC) to score binary AlphaFold multimer (AF-M) predictions by structural quality and consistency with experimental omics datasets. www.cell.com/molecular-ce...

PhD student recruitment 2025 PhD recruitment is now open!

Our spring round of PhD recruitment is now open!

We’re looking for researchers of any nationality with backgrounds in biological or biomedical sciences, physics, chemistry, maths or computer science.

Find out more and apply by midday, 19 March:

www.crick.ac.uk/careers-stud...

4-year PhD studentship available in my lab to study helper helicase function in eukaryotic DNA replication. Happy to receive any enquiries directly to me. Come join us and add your figure to the lab lego collection!

institute-genetics-cancer.ed.ac.uk/igc-graduate...

Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield on FindAPhD.com PhD Project - Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield, listed on FindAPhD.com

Please share: Still plenty of time to apply for PhD projects in my lab using single-molecule methods to look at disordered regions and condensin complexes!
Contact me at e.cutts@sheffield.ac.uk
For details on how to apply: www.findaphd.com/phds/project...

Opening for a senior postdoc in our single-molecule DNA replication lab! Deadline January 15, 2025!

CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.

The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...

I have received a Wellcome Discovery Award to study the initiation of eukaryotic DNA replication, with 8 years of funding at the Francis Crick Institute.

Looking for a postdoc in molecular mechanisms, cryo-EM, cryo-FIB and in situ structure? Contact me now!

3 postdoc positions advertised soon.

Congratulations, Gideon!

📢 Happy to share our bioRxiv preprint on how CRL4-DCAF12 regulates the cellular level of MCMBP, a chaperone of nascent MCM2-7 complexes, to ensure error-free genome duplication. The full story can be found here: lnkd.in/eCjVNQks

Two fully funded PhD opportunities in our lab. Interdisciplinary, DNA replication, cancer research, structural biology… deadlines in early Jan. Open to international students. t.co/WEJJIU4zU4

www.findaphd.com/phds/project...

Happy to share our latest work🍾 🥳

PhD student Sophie Dürauer discovered together with many wonderful collaborators that the ubiquitylation of DNA-protein crosslinks promotes their cleavage by SPRTN more than 100-fold 🧬🔧

Super duper resolution fluorescence microscopy 🤩

Made a new starter pack for labs developing and applying advanced microscopy for biological discovery. Definitely still missing loads of labs, write me to be added.
go.bsky.app/SHQCnEe