Yongdeng Zhang

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods 4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves near-isotropic resolution. @yongdengzhang.bsky.social

www.nature.com/articles/s41...

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods 4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt

Congrats!

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.

▶️ www.biorxiv.org/content/10.1...

🚨 2 × PhD positions @EPFL! 🚨
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → fschueder@ethz.ch
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Congrats Rita! Maybe my next paper will be in NBME!

Array detection enables large localization range for simple and robust MINFLUX - Light: Science & Applications Light: Science & Applications - Array detection enables large localization range for simple and robust MINFLUX

Excited to share our paper on ISM-FLUX, our new #MINFLUX-based technology. Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6 www.nature.com/articles/s41...

This has been one of my favorite ER images ever since I first saw it in Jennifer's presentation.

Congratulations!!!

Direct-view oblique plane microscopy The ability to rapidly image mesoscopic samples is critically important for many areas of biological research. Owing to its high throughput and gentle, volumetric imaging nature, light-sheet fluoresce...

We present:

Direct-view oblique plane microscopy

www.biorxiv.org/content/10.1...

This is fantastic！

Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM - Nature Methods The 4Pi-SIM microscope combines the benefits of structured illumination microscopy with 4Pi geometry for improved axial resolution, offering isotropic, multicolor super-resolution imaging of dynamic p...

Incredible new work from Yongdeng Zhang demonstrating live 4Pi SIM--and in two colors! www.nature.com/articles/s41...