Ran Tahan

Phage-associated Cas12p nucleases require binding to bacterial thioredoxin for activation and cleavage of target DNA www.nature.com/articles/s41...

04.01.2026 05:56 — 👍 18    🔁 8    💬 0    📌 0

Inferring single-cell heterogeneity of bacteriophage lysis-associated life-history traits from population-scale dynamics www.biorxiv.org/content/10.1...

24.11.2025 04:39 — 👍 5    🔁 2    💬 0    📌 0

A functional cyanophage thioredoxin increases competitive phage fitness www.biorxiv.org/content/10.1... @rantahan.bsky.social

24.11.2025 04:35 — 👍 5    🔁 2    💬 0    📌 0

the sun is shining through the clouds in the sky above the ocean ALT: the sun is shining through the clouds in the sky above the ocean

Viral NblA proteins negatively affect oceanic cyanobacterial photosynthesis www.nature.com/articles/s41...
this project was led by @omernadel.bsky.social and is a joint work between the labs of @bejalab.bsky.social, Debbie Lindell and Oded Kleifeld from @biologytechnion.bsky.social

12.11.2025 16:00 — 👍 44    🔁 27    💬 9    📌 2

@bejalab.bsky.social

12.11.2025 10:02 — 👍 0    🔁 0    💬 0    📌 0

A functional cyanophage thioredoxin increases competitive phage fitness Thioredoxins are ubiquitous redox proteins that are found in all domains of life. These conserved proteins are also found in many phages, including marine cyanophages that infect the ecologically important marine cyanobacteria. However, their role in phage infection is not known. Cyanophages also carry many small genes lacking homology to known functional domains. Whether these have a functional role or not remains unknown. Here, we explore the distribution and role of a cyanophage thioredoxin (trxA), and that of a small gene directly downstream of it (g26), in phage infection. For this we used the T7-like cyanophage, Syn5, which infects an open-ocean marine Synechococcus strain, WH8109. We found that thioredoxin genes are common in phage genomes, including in cyanophages. The g26 gene, however, is restricted in it distribution to the cyanophages. The cyanophage thioredoxin is catalytically active and it increases phage DNA replication, progeny production and competitive fitness. It also negatively impacts host growth. The g26 gene product is translationally coupled to, and thus dependent on, translation of the thioredoxin gene. This gene itself significantly increases phage virulence and fitness, yet reduces burst size. Our findings demonstrate that cyanophage thioredoxins impact phage fitness and infection physiology and that small viral genes with no homology to known genes can play an important role in the infection process. These findings provide insights into the importance of unusual genes in phage genomes and show that they are likely to play an important role in the interactions between abundant cyanobacteria and cyanophages in ocean ecosystems. ### Competing Interest Statement The authors have declared no competing interest.

A functional cyanophage thioredoxin increases competitive phage fitness | bioRxiv https://www.biorxiv.org/content/10.1101/2025.10.05.680603v1

07.10.2025 01:24 — 👍 11    🔁 4    💬 1    📌 0