David Mick

Another fantastic technology development paper by @davidbreslowlab.bsky.social. Being able to perform microscopy-based CRISPR screens on fixed cells is big. And TZMP1 is a fascinating transition zone proteins. We may be missing many more of these #microproteins. Congrats to all authors!

Thanks Martina! :-)

5) Thanks again to all authors, especially Mick lab grad student Tommy Sroka and PostDoc Avishek Prasai, as well as valetrivi.bsky.social and Josefine Hoeren from the Feistel lab who established iAPEX in developing Xenopus brains.

4) iAPEX is more specific than conventional APEX labeling, which is a huge deal for subcellular proteomics of small subdomains. We profiled primary cilia of two different cell types and identify new cilia candidate proteins. Our data confirms heterogeneity in cilia proteomes of different cell types.

3) Our collaborator @biancaschrul.bsky.social demonstrated this by selectively activating a UBXD8-population that localizes on the surface of #LipidDroplets. We think this approach has lots of potential to investigate organellar contact sites.

2) We can use the iAPEX enzymatic cascade to activate subpopulations of APEX enzymes in subcellular compartments, specifically where they co-localize with DAAO. We can show this for #mitochondria, subdomains of cilia (cilia tips), as well as for proteins that localize to two different compartments.

1) By using a D-amino acid oxidase (DAAO), iAPEX omits the requirement of external H2O2 addition and therefore reduces toxicity and allows in vivo applications, which our fantastic collaborators @kerstinfeistel.bsky.social and @walenteklab.bsky.social could show in developing #Xenopus embryos.

An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, app...

Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...

Congrats all, in particular Rudi! Love the paper.

Everybody in my lab is working so hard, lots of things can happen within two days.

Rethinking Europe’s Forests: Why Tree Species Matter for Climate Cooling Yao et al. show that in Europe, forestation’s climate benefits depend on tree species. Converting conifers to broadleaves can reduce summer heat extremes and reverse warming effects of afforestation, ...

"... the species of trees we choose to nurture may be just as important as the number of trees we plant." Read the blog post behind the paper by Yao et al. regarding the role of #tree species in the #climate impact of European #forests. communities.springernature.com/posts/rethin...

Amazing work by the Breslow lab. Super excited to finally see this published. Unbiased CRISPRa screening reveals a novel and unexpected cilia disassembly pathway associated with neurological disease (focal cortical dysplasia). Must read for both #cilia and screening technology enthusiasts.

It was a lot of fun to write this review on cilia proteimics with Bernhard Schermer @cilib.social.cologne.ap.brid.gy and Ronald Ropeman, and we are excited to see it published in the @jcellsci.bsky.social special issue on Cilia and Flagella: from basic Biology to Disease.

Thank you all for a wonderful meeting full of amazing science and inspiring discussions. It was so much fun! Thanks @bastinlab.bsky.social, Sylvie Friant and Séverine Bär for organizing it at such a beautiful location. Can't wait for next time in Lyon!

Loss of ciliary proteins IFT20 and IFT88 results in defective phagocytosis and metabolism in the RPE A major proportion of retinal disease-causing genes are related to the primary cilium, a microtubule-based signalling organelle essential for multiple developmental pathways. Previous work has shown t...

Interesting connection between primary #cilia and energy #metabolism in #mitochondria. www.biorxiv.org/content/10.1...

Very much looking forward to our next focus workshop on #cilia and #centrosomes. We'll have fantastic speakers again.

Professor Jochen Guck, Director at the Max Planck Institute for the Science of Light (MPL), He headed the division “Cell Physics” at MPL and the Max-Planck-Zentrum für Physik und Medizin (MPZPM).

With deep sadness, we share that Prof. Jochen Guck, Director at the Max Planck Institute for the Science of Light passed away on October 3. His visionary work bridged physics and medicine. We have lost a brilliant scientist and dear friend.

mpl.mpg.de/news/article...
@fau.de

📸 Stephan Spangenberg

An ectopic Hedgehog signaling axis drives directional tumor outgrowth in a mouse model of hereditary multiple osteochondromas Redeployment of a developmental program drives the outgrowth of bone tumors in an inherited disease.

New findings in mice cast light on the pathways behind the growth of painful bone tumors in hereditary multiple osteochondroma and suggest that these mechanisms could be targeted to slow the disease’s progression.

Learn more in #ScienceSignaling: https://scim.ag/46VRSIp

We’re renewing our collaboration with Google DeepMind!

We'll keep developing the AlphaFold Database to support protein science worldwide 🎉

To mark the moment we’ve synchronised the database with UniProtKB release 2025_03.

www.ebi.ac.uk/about/news/t...

🖥️🧬 #AlphaFold
@pdbeurope.bsky.social

Deeply saddened to hear about the passing of Jochen. We had very few but absolutely fantastic interactions when he was still in Dresden. He will be missed dearly.

Today is the #BBSGlobalDay — an opportunity to raise awareness of this rare condition and offer support to everyone living with BBS and their loved ones 🌍 🌎 🌏
www.img.cas.cz/2025/10/8758...

Can I invite myself?

First pizza lunch after a whirlwind week moving into our brand-new research building. Exhausted, but absolutely thrilled by the incredible facilities & lab space. Can’t wait to dive back into science.
A few benches are still waiting for curious minds: PhD students and postdocs, come join us! 😉

Monoclonal antibodies revolutionized biomedical science and health care The story of the discovery of these molecules 50 years ago shows how the free exchange of ideas can lead to life-changing treatments.

'This story shows that it is possible to establish a multibillion-dollar industry purely by letting scientists communicate their research, exchange information and share materials.'
www.nature.com/articles/d41...

Science doesn’t need to be pretty and go according to plan; it just needs to lead to a discovery. If doesn’t have to be done alone or together with someone else; there just needs to be a discovery. It doesn’t need to happen fast or slow; just as long as there’s a discovery, then everybody is happy.

Thrilled to see our study on how kinesin-2 motors are switched on and off published in @natsmb.nature.com ⚛️

➡️ www.nature.com/articles/s41...

Congrats to all authors from me and Anthony 🎉 @dunnschool.bsky.social Check out this animation made by talented PhD student @matthew-batisio.bsky.social 😆

Head of the Cell Biology and Biophysics Unit The European Molecular Biology Laboratory (EMBL) is Europe’s flagship life sciences organisation, and one of the world’s leading research institutions. An intergovernmental organisation of 29 Member S...

EMBL Cell Biology and Biophysics department is searching for a new director. A major position to lead this field in Europe and beyond.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...

This is figure 2 showing how SNAP-tag engineering is aiming for faster and brighter fluorescence labeling

SNAP-tag is a widespread tool for labelling protein for bioimaging. An article published in Nature Chemical Biology presents SNAP-tag2 with increased labelling kinetics and brightness, which translates into a better performance in live-cell super-resolution imaging. go.nature.com/46fMIZd 🧪

Molecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical Biology Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.

Beautiful work on the development of protein kinase reporters. Congrats all! www.nature.com/articles/s41...

Grateful to be part of such a fantastic consortium! Huge thanks to our amazing coordinator Christina and to Silke for organizing an inspiring annual meeting in Jena. Latest new: we’ve got a new rising star on the team! #cilia