A.Bosson

Just out in Nature! “The astrocytic ensemble acts as a multiday trace to stabilize memory.” We identified astrocytic ensembles that link experiences across days to stabilize memory nature.com/articles/s41.... New astrocyte tools are openly available at Addgene: addgene.org/Jun_Nagai/. 1/8

Étude réalisée à Montréal | L’impact du stress sur le cerveau dépendrait du sexe Le cerveau des mâles et des femelles ne réagit pas de la même manière au stress, démontrent pour la première fois des travaux réalisés à Montréal.

L’impact du stress sur le cerveau dépendrait du sexe www.lapresse.ca/actualites/s...

Thrilled to share our new study showing microglia prune astrocyte processes in adult brain, to regulate synaptic transmission, hypothalamic neuron activity, and salt-induced hypertension in🐀. Huge team effort - congratulations to all🥂🍾🎉

Go Lewis 👏👏👏

Sleep loss is a metabolic disorder Sleep deprivation shifts metabolic resources and deprioritizes processes that support cognition.

Sleep loss is a metabolic disorder

"This review describes how sleep deprivation increases resting energy expenditure, leading to the development of a negative energy balance in highly active cells like neurons."

www.science.org/doi/10.1126/...

Overall, this heterogeneity shows astrocytic functional adaptations to synaptic circuits within CA1.

Thanks to everyone involved.

Despite this, both astrocyte populations rely on the eCB system to maintain iLTD at the postsynapse.
Possibly underlying a fundamental property of CA1 astrocytes to regulate pyramidal cells inhibition.
Finally, the 2 astrocyte populations are also reflected in their gene expression profiles

Combining chemo- and optogenetics with dual patch-clamp revealed heterogeneous synaptic regulation, mirroring the spatial segregation of astrocytes and interneurons. We found that SP astrocytes exclusively modulate PV related inhibition while SR astrocytes exclusively affect SOM related inhibition

Using GCaMP and dye-filling, we uncovered syncytial and calcium dynamics heterogeneity between SP and SR astrocytes. Ca2+ activities progressively shifted along the SP–SR axis, with a threshold at 20 μm from stratum pyramidale border.

Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells - Nature Communications Astrocytes have been shown to regulate glutamatergic transmission in the brain. Here, the authors show that astrocytes also detect and modulate GABAergic transmission from somatostatin but not parvalb...

Following on the finding that stratum radiatum astrocytes upregulate SOM inhibition (but not PV inhibition) onto pyramidal cells(www.nature.com/articles/s41...), I wondered whether this could be tied to heterogeneous astrocyte populations within CA1. Meeting the needs of diverse synaptic environment

Happy to see that the 2nd part (2018 to 2021) of my postdoc work is now out as a preprint.

@lewisholt.bsky.social sharing some cool data about astrocytes driving stress-induced behaviour! #astroglia

Astrocytes This book gathers methods essential for investigating the multifaceted roles of astrocytes in both physiology and pathological conditions.

Very happy to share several common protocols used in our lab for the second edition of the book "Astrocytes: Methods and Protocols"
@lewisholt.bsky.social @astroantho.bsky.social

Thanks @dibenedetto-lab.bsky.social for being a very patient editor!

link.springer.com/book/10.1007...

Poster showing details for seminar from Dr Jun Nagai. Principal Investigator Team Leader at RIKEN Centre for Brain Science Tokyo, Japan Title: Behaviorally relevant astrocyte ensembles for systems stabilization Le conférence seront présentées en anglais Le mercredi 2 Avril 2025 - Wednesday 2nd April 2025 at 12pm Locale : R05.212B

Montréal glia people / neuroscience community - I'm hosting Jun Nagai (RIKEN) on April 2nd at the @crchum.bsky.social
If you're interested see details below, or reach out to me! Open to all.

Two-photon image showing astrocytes with stained with SR101 in magenta, membrane tethered-GCaMP in cyan

Not sure if our 'striking image' we submitted will get used but here it is. Super striking, if you ask me!

Credit - @mathiasgua.bsky.social

Very honoured to be named the researcher of the month by SENSUM - the mental health and neuroscience strategy at @umontreal.ca

www.sensum.umontreal.ca/en/2025/01/3...

A spatial threshold for astrocyte calcium surge In astrocytes, there exists an intrinsic spatial threshold of subcellular calcium levels that triggers an astrocyte calcium surge throughout the cell, demonstrating cellular astrocyte calcium integrat...

Astrocyte physiologists and computationalists!

Imaging astrocyte structure and function, we discovered and quantified a spatial threshold to astrocyte calcium surge ⭐

doi.org/10.7554/eLif...

Sped up*

Same here :)
Yes spontaneous activities in acute slice. It's been speed up for aesthetics here, initially it is a 7 min recording.

More folks will realize how beautiful astrocytes are 🤩

A touch of blue velvet LUT with this #FluorescenceFriday

GCaMP8 astrocytic activities at the 2p

How convenient - this tree already comes with ornaments! 🎄🧪

Get into the holiday spirit with this beautiful cell tree by @oozguc.bsky.social from @ibecbarcelona.eu. This beautiful fusion of science and art was made using our Lamin B1 stem cell line!

What would you want to see under your cell tree?

2 eGFP #astrocytes contacting a blood vessel with their arborization almost looking like a christmas tree for this #fluorescencefriday #astrocyte

Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation Early-life stress can have lifelong consequences, enhancing stress susceptibility and resulting in behavioural and cognitive deficits. While the effects of early-life stress on neuronal function have ...

Delighted to share an update to our preprint, reviewed at @elife.bsky.social, and now sent back for re-review.
Colossal work by the team and driven home by @mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...

we haven't talked about this in the SKY>

exciting preprint from Kevin Guttenplan in Marc Freeman's lab at @volluminstitute.bsky.social

Adrenergic signaling gates astrocyte responsiveness to neurotransmitters and control of neuronal activity

www.biorxiv.org/content/10.1...

Point of View: Journal clubs in the time of preprints Early-career researchers can learn about peer review by discussing preprints at journal clubs and sending feedback to the authors.

10/ Why should you bring #preprints into your journal club?

Here’s what #ECRs stand to gain by looking beyond published papers.
https://buff.ly/3VbvNjT

Microglial engulfing of glutamatergic inputs and diminished excitability of D1 medium spiny neurons of the nucleus accumbens by peripheral inflammatory insult Neuroimmune responses to systemic inflammation can generate anxiodepressive behaviors and psychomotor slowing. The nucleus accumbens (NAc) is a neural hub encoding mood and motivation that contributes...

New paper from our neighbours Stephanie Fulton’s lab investigating microglia, inflammation and synaptic transmission in the NAc.
www.biorxiv.org/content/10.1...

some great starter packs for the #glia, #astrocyte, #oligodendrocyte, #microglia, #neuro researcher: gliasky, astrocytesky, microglia
But if you also like interactions between celltypes, please help this starter pack get started. Maybe someone can connect all of us in a feed?

go.bsky.app/MHtJzeC

Great one ! I think we can even spot a supernova remnant from 2011 in the white square

Not sure I have any good pictures in the back pocket to beat the calcium imaging from @mathiasgua.bsky.social

Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!