John Ridenour

NIH Grants Fueled $95 Billion In FY 2024 Economic Activity, Finds New Report National Institutes of Health grants generated almost $95 billion in economic activity nationwide in FY 2024 according to a new report by United for Medical Research.

New report shows that NIH grants fueled $95 billion in economic activity and 407,782 jobs in 2024.

That's not to mention the countless lives that biomedical research has saved.

Show me a better investment than that.
www.forbes.com/sites/michae...

Zachary Lewis on LinkedIn: Laboratory Research Technician We have open positions for a lab manger and a laboratory technician. Applications from displaced federal employees are welcome. Lab manager position:…

Two open positions for a lab manger and a laboratory technician. Applications from displaced federal employees are welcome.

Lab manager position: www.ugajobsearch.com/postings/410...
Lab tech position: www.ugajobsearch.com/postings/391...

Please email zlewis@uga.edu if you have questions

Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA Quiescence in Saccharomyces cerevisiae is a reversible G0 crucial for long-term survival under nutrient-deprived conditions. During quiescence, the genome is hypoacetylated and chromatin undergoes sig...

a short and sweet paper in which we use 3D STED and Micro-C analysis to show dramatic changes in large 3D chromatin structures in quiescence. www.biorxiv.org/content/10.1...

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2

A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes https://www.biorxiv.org/content/10.1101/2024.12.06.627179v1

Remarkable structural insights from Carl Wu lab: SWR1 complex has a mechanism for sensing open DNA regions to insert H2A.Z-nucleosomes. CryoEM is transforming structural biology:
www.cell.com/cell/fulltex...

Delighted to share my Current Opinions review on nucleosome remodelers in DNA repair, co-authored with Jessica Downs: an uptodate survey of R-loops, 3D organization and remodelers especially jn DSB repair. Enjoy!

article link:
authors.elsevier.com/sd/article/S...

On my reading list, a incredible work again by the Xu lab #cryoEM

www.science.org/doi/10.1126/...

Structure of Rpd3S-nucleosome complex.

Our paper on the structure of the complete Rpd3S-nucleosome complex is now out in Nat Comm: rdcu.be/ds6k1 . Congrats to @jonmarkert.bsky.social
and co-corresponding author Seychelle Vos! This is the second HDAC-nucleosome structure from Jon this year. Amazing!

TODAY is your LAST CHANCE to submit an abstract for #Fungal24

#Fungal24 abstracts and early registrations are due today, December 5, 2023 at 8:00 p.m. EST.

community.alliancegenome.org/t/today-is-l...

This looks very interesting. 🧬🔄🧬

Excited to share my postdoc work, REMORA (RNA-encoded molecular recording in adenosines), now officially online in Nature Methods: www.nature.com/articles/s41... (1/7)

The Fusarium Workshop is now open for registration at the Fungal Genetics Conference 🥳 Looking forward to co-chairing with Tom
Baldwin and Domingo Martinez-Soto! genetics-gsa.org/fungal-2024/...

Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitiv... bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

The hacked the @nanopore sequencer to read amino acids and PTMs along protein strands. This opens up the possibility for barcode sequencing at the protein level for highly multiplexed assays, PTM monitoring, and protein identification 🧬🧪🤯
www.biorxiv.org/content/10.1...

Invited Speakers - 32nd Fungal Genetics Conference Visit our website to learn more.

Registration has started for the Fungal Genetics Conference 2024! Looking forward to organizing the session ‘Chromatin and Chromosome Biology‘ with Mareike Möller, many thanks to the organizers Michael Freitag and Natalia Requena for this opportunity! genetics-gsa.org/fungal-2024/...

Nucleus showing Pol II cluster at the histone locus

📣 New preprint! Pol II forms clusters, some of which are giant: Pol II clusters at fly histone genes could fill a yeast nucleus!👇How do these structures regulate transcription? This is what
Feiyue Lu, a joint postdoc w Ruth Lehmann, looked into 1/6 biorxiv.org/content/10.1...

ChEC-seq2: an improved Chromatin Endogenous Cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein-DNA interactions https://www.biorxiv.org/content/10.1101/2023.10.15.562421v1 Defining the in vivo DNA binding specificity of transcription factors (TFs) has relied nearly exclus

ChEC-seq2: an improved Chromatin Endogenous Cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein-DNA interactions https://www.biorxiv.org/content/10.1101/2023.10.15.562421v1

Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells - Nature Methods A comprehensive redevelopment of the ribosome profiling workflow involves improved nuclease treatment and sequencing library preparation, enabling richer and more accurate translatome profiling with l...

#RNABiology recent highlight, a thoroughly revised and streamlined RiboSeq method from @nickingolia.bsky.social

Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells. doi.org/10.1038/s415...

Nanopore sequencing of DNA-barcoded probes for highly multiplexed detection of microRNA, proteins an... This study establishes a method for multiplexed detection of up to 40 different biomarkers in a single assay by combining nanopore sequencing with analyte-selective barcoded probes, including cardiac ...

A clever approach because it combines Oxfordn nanopore sequencing with barcoded molecular probes to achieve highly multiplexed and sensitive detection of various biomolecules in a single sample.
www.nature.com/articles/s41...

Can’t begin to express my profound gratitude to each and every one of these people for making the first edition of the New Investigators in Chromatin and Epigenetics (NICE) conference so stellar! Particularly big 🙏 to my co-organizers @hainerlab.bsky.social and @4everBiochemist

Heading to the inaugural NICE meeting: www.nicechromatin.com
A chromatin&epigenetics mtg for new PIs only (pre-tenure); we are hoping to expand pending how this initial mtg goes! if you are interested in next year, get in touch! We want to build a chromatin community filled nice (get it?!) folks!

Nowadays nanopore can call m6A, which means we can do ChIP-seq-like experiments using DNA adenine methyltransferase and feed it to nanopore. Figure from: genomebiology.biomedcentral.com/articles/10.... Note that they used EcoGII (no sequence specificity) instead of Dam.

Very cool. Never knew it was from the Hutch.

I've been dogmatic about not capitalising the w in "western blot": Southern blot is named after a person, so should be, but northern and western aren't.

Here Burnette certainly capitalises "Western". Hmm. Wiki doesn't.

bioRxiv goes big on Bluesky: accounts for all subject categories now available (Please Reskeet) connect.biorxiv.org/news/2023/09...

Differential Modulation of Polycomb-Associated Histone Marks by cBAF, pBAF, and gBAF Complexes bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

This is cool @kirklandlab.bsky.social - Really intrigued by the ability to specifically recruit c/p/g BAF complexes! 🧬🔄 www.biorxiv.org/content/10.1...

Exactly! These methods work most of the time but not always.

Chemical-genetics refines transcription factor regulatory circuits Transcriptional dysregulation is a key step in oncogenesis, but our understanding of transcriptional control has relied on genetic approaches that are…

www.sciencedirect.com/science/arti...

Skeet @ labnotes.org Find your Twitter/Mastodon follows on BlueSky

Hey newcomers! You can find the folks you followed on Twitter with this tool (it’s imperfect but useful!) skeet.labnotes.org