Guillaume Gaullier

Not even: is some crystal structures (I think including the original 1AOI), the tail of H4 binds to the acidic patch of another nucleosome in the neighboring unit cell. 🧬🙃🧬

Some pioneer TFs.
A good review about factors binding to the acidic patch: doi.org/10.1016/j.sb...
A few years ago I looked for a conserved acidic patch binding sequence motif, but found that there didn't seem to be any: the only common residue was the Arg anchor. Maybe time to revisit this?

HHMI adopts Plan U journals.plos.org/plosbiology/...

Naiavirus: an enveloped giant virus with a pleomorphic, flexible tail - Nature Communications Rodrigues and Queiroz et al. report the discovery of Naiavirus, the largest enveloped virus, isolated from a Brazil biome. With an oval capsid and flexible tail, its giant genome holds many novel gene...

@nucleosomepolice.bsky.social do you think this newly discovered giant virus has nucleosomes? doi.org/10.1038/s414...

This is impressive! And very helpful to the whole community, so thanks a lot for taking the time to make it work and sharing the results!
(But also not thank you: I really didn't need one more distraction in a busy fall semester... 😅 I have a dataset with a very small protein lying around...)

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

Great point. Make it opt out, not opt in, for authors to see feedback.

We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose.

How can we do that if public reviews are not even seen by the authors?

Why hasn't Preprint Peer Review caught on more?

I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs.

more in thread below!

How exactly do you set up the remove duplicates job to answer this question?
I have a similar case: different conformations of a hexameric (D3) complex, and I'd like to know if the different conformations of the subunits (found by symexp and 3D classification) can occur in the same hexamer or not.

Thanks for sharing @olibclarke.bsky.social. I wonder if many more "hollow" complexes are co-purified with binding-partners. Let's all put more datasets on EMPIAR to revisit processing with new creative approaches like Oli's.

It's one of the best time investments I've made. Great for making figures, posters, or any visual really. It even has a tool to make slides! (which produces a file that opens in a web browser, so can be displayed on any computer regardless of which presentation software is available).

Simple drawings are fine. But if you want to re-use and adapt your drawings later, you should really use a vector format, so not Paint nor Gimp (both raster editors). Inkscape is an excellent vector drawing program: inkscape.org
A bunch of others are listed here: en.wikipedia.org/wiki/Compari...

www.jiscmail.ac.uk/cgi-bin/wa-j...

Post this to CCPEM tomorrow. 👌 🍿

And in New Zealand too. I was trying to guess on the same continent. 😉

Mountains so close to the sea = somewhere in the Pacific Northwest? WA or BC?
Looks amazing either way, enjoy! 🤩

But... but... I was not even trying! 😅😅😅

Read this elsewhere, can't remember who said it first, but thought it was really good:

They should do their assignments without AI for the same reason they don't use a forklift to lift weights at the gym. It's not getting the weights to higher elevation that matters, it's the effect it has on you.

In the Bay Area? Love computational cryo-EM/ET?

Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!

Details and registration at frostbyte-cryoem.github.io

Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

Open positions Postdoctoral scholar in biochemistry of cyanobacterial CO 2 fixation The Blikstad research group is searching for a postdoc to study fundamental principles of CO 2 fixation in cyanobacteria. Our...

Come join us as a postdoc! We have an open position in biochemistry of cyanobacterial CO2 fixation focusing on regulation of carboxysome function! Please help me spread the word and welcome to apply! www.blikstadlab.org/open-positio...

Or don't tie people's careers to the number of papers they publish? If there is no longer an incentive to maximize this number, it will become viable to spend more time on fewer, more solid papers. Fewer papers also means it's easier for all to stay up-to-date with the state of the art.

How to self-host all of Bluesky (except the AppView (for now)) 33 comments

It is technically possible, but seems much more costly than running a Mastodon instance. See some messages in this discussion (and the article being discussed for technical details): lobste.rs/s/gsj2e2/how...

The CCPEM Spring Symposium at the end of April in Nottingham, UK.
It became fully hybrid after getting back to in-person, so you can watch all talks online as they happen.

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Well that's one more reason to choose a fediverse instance that doesn't have this limitation. 😉

This is not my observation. I just checked, and I can search, view, list and follow Threads accounts from my Mastodon instance. Haven't tried interacting with one, but I don't see why it wouldn't work if I can successfully follow (this I confirmed).

Some billionaire might well buy Bsky before the next election...

Is it me or is this place really taking off?
Shall we all take a step closer to full sustainable independence and try Mastodon too!

I could tolerate only one "Twitter cycle". Maybe you can take two and are betting on Bsky for now. The median person can maybe take a few more. But eventually this nonsense will wear out everyone who simply wants to communicate with others.