Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
26.09.2025 02:28 — 👍 57 🔁 24 💬 1 📌 2
Lattices is an optimal way of packing so perhaps it just packs this way to cram as many as possible on the membrane in a stable way....Anyways, always impressed with the Engel lab papers! In-situ structure work is the best kind of in-situ
24.09.2025 18:22 — 👍 1 🔁 0 💬 1 📌 0
A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾
20.09.2025 19:24 — 👍 136 🔁 46 💬 3 📌 0
Very impressive tomograms. Those lattices on the thylakoid membranes...could almost do 2D xtallography...Beautiful work!
21.09.2025 22:18 — 👍 1 🔁 0 💬 1 📌 0
It has also worked for us for a small protein without using any scaffolding. Although we are careful with the model, we are quite impressed by the map quality achieved as we were able to dock it a refine it pretty well. With that said, we are cautious and eager to hear the whole story :)
21.09.2025 22:15 — 👍 1 🔁 0 💬 0 📌 0
Absolutely!
21.09.2025 22:12 — 👍 1 🔁 0 💬 0 📌 0
Very interesting. Thanks
13.09.2025 18:52 — 👍 1 🔁 0 💬 0 📌 0
Love the drawing!
24.08.2025 01:40 — 👍 3 🔁 0 💬 1 📌 0
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
23.08.2025 23:21 — 👍 88 🔁 27 💬 2 📌 0
Research
Xu Lab of Computational Biology
New Title Alert: AITom- an open-source platform for AI driven cellular electron cryo-tomography analysis.
Learn more here: buff.ly/LLGhjvY
#SBGrid #SBGridSoftware #StructuralBiology
12.08.2025 16:03 — 👍 4 🔁 4 💬 0 📌 0
I tried that but what worked the best was to germinate on agar germinating medium, then resuspend them gently and pipette them on grid. Not trivial but doable.
30.07.2025 04:14 — 👍 0 🔁 0 💬 0 📌 0
Congratulations!
29.07.2025 20:05 — 👍 2 🔁 0 💬 1 📌 0
New preprint: In situ Architecture of #Plasmodesmata.
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
26.07.2025 15:38 — 👍 197 🔁 72 💬 9 📌 8
Our methods paper made it to bioRxiv:
doi.org/10.1101/2025...
Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM
19.02.2025 06:06 — 👍 118 🔁 43 💬 2 📌 5
Great news ! Will give it a look. The tomograms look very nice!
I will release the data on the CZI public tomography portal
29.07.2025 20:03 — 👍 1 🔁 0 💬 1 📌 0
'cux good data don't wait!
24.07.2025 16:11 — 👍 2 🔁 0 💬 0 📌 0
Outstanding work from Christine, Stan and the whole team on this beautiful story. Wow.
17.07.2025 13:44 — 👍 5 🔁 1 💬 0 📌 0
Reminds me of the South Park episode where Randy tries to do the biggest poop in the world
16.07.2025 23:06 — 👍 0 🔁 0 💬 1 📌 0
C'est a Arcachon ça ? Sur la promenade ? J'ai aussi cru a une vrai baleine. Mais vu les couleurs quand je me suis rapproché....
16.07.2025 21:18 — 👍 1 🔁 0 💬 0 📌 0
Big incel power tripping vibs right here. Dangerous when those kinds of people hold that kind of power.
14.07.2025 06:40 — 👍 0 🔁 0 💬 0 📌 0
Yup. Have been saying this since Trump started overstepping. Where are all the wannabe heroes doing CQB training on weekends to prepare against the evil government? Prob no where because they are on the good side of the fence...for now...
13.07.2025 06:21 — 👍 2 🔁 0 💬 0 📌 0
When I was at UCLA, I started a similar project on hex1 bio crystals in fungus to solve by fib milling microED. Code name of the project was also IncelED 😂 odd coincidence!!
13.07.2025 03:44 — 👍 1 🔁 0 💬 0 📌 0
The maturity of a 5yo basically. Arrested development?
13.07.2025 03:34 — 👍 0 🔁 0 💬 0 📌 0
They are paid crap salary to do gestapo work too. They must really love the hunt.
10.07.2025 04:57 — 👍 2 🔁 0 💬 2 📌 0
Neural net picker with a truly general model - no per dataset training required (at least if it works as advertised). Want to try this on some messy/mixed samples where other approaches struggle!
09.07.2025 13:03 — 👍 6 🔁 2 💬 1 📌 0
Walkthroughs on how to apply protein design to cryo-EM (if you have experience in doing so). Please 🙏
30.06.2025 23:17 — 👍 1 🔁 0 💬 1 📌 0
Running Phenix and stuck?
Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program.
Big thanks to Tom Willinger for making this happen!
Try it out: phenix-online.org/version_docs...
23.06.2025 05:14 — 👍 27 🔁 10 💬 1 📌 0
Frostbyte 🥶 socks provided by @hammerspace.com
16.06.2025 15:48 — 👍 2 🔁 0 💬 0 📌 0
It was really fun! Do you mind linking to where all your python scripts for cryoEM are ?
13.06.2025 00:32 — 👍 0 🔁 0 💬 1 📌 0
Mathematics Sorceror (sensory alchemist) at the Arctangent Transpetroglyphics Algra Laboratory (ATAL), I transflarnx mathematics into living rainbows. http://owen.maresh.info https://github.com/graveolensa
Psoeppe-Tlaxtlal, (an undreamt splendour?)
Improving accessibility of TEM and cryo-TEM in research and industry.
CNRS researcher at IGFL, ENS-Lyon.
Ecology-Evolution-Development
Nature Photography
website: https://arnaudbadiane.com/
CryoEM, membrane proteins and whatnot
An expedition to study coastal ecosystems and their response to the environment, from molecules to communities
Facts & strategy, in an authoritarian takeover.
Rightwing billionaires want to privatize NIH and use it to control universities.
We work to cure diseases like cancer.
Pers views. #science #medicine
European Research Council, set up by the EU, funds top researchers of any nationality, helping them pursue great ideas at the frontiers of knowledge. #HorizonEU
Cryo-electron tomography and subtomogram averaging. Pushing buttons, turning knobs, and furiously smashing keyboard. Assistant prof at Vanderbilt University; I ask other people to pipette things, often viruses.
Staff Research Associate @ UC Davis
🌱🍇🧪🧬🖥️ #PlantScience #Genomics
Grape (pan)genomics
Scripps Research Professor. HHMI Investigator. Nobel Prize Physiology or Medicine 2021. Opinions my own.
🔬 CNRS Research Director, Bordeaux
| Structural biologist & microbiologist
🚴♂️ Cyclist (mostly Velotaf) & runner (just for fun)
⛰️ Mountain & nature lover
🎓 UCL/Birkbeck & Institut Pasteur alumnus
🔬 fronzeslab.cnrs.fr
#cryoEM #cryoET #microsky
cellarchlab.com Univ. of Basel Biozentrum🇨🇭. Exploring molecular architecture inside cells with #CryoEM #TeamTomo. Plants and algae in a changing climate. ❄🔬 OF 🌿 4THE 🌍!
Joint Head of Structural Studies at @mrclmb.bsky.social. Develops & uses #cryoEM to study amyloids in neurodegeneration. #tau, #alphasynuclein, #opensoftware, #RELION. All opinions my own.
Scientist, 2017 Chemistry Nobel Prize (math and computational methods underlying cryo-EM of biological molecules), fiction writer, photographer, gardener.
Climate change, science education, fight of fascism
franxfiction.com and joachimfranklab.org
Assistant Professor at Utrecht University 🇳🇱
We use #cryoEM and molecular #virology techniques to study the infection mechanisms of +ssRNA viruses.
https://www.uu.nl/medewerkers/DLHurdiss
Views are my own.
Molecular biologist. Group leader at MRC LMB Cambridge UK. Fellow at Clare Hall college. #CryoEM #RNAbiology #DNArepair
Views/opinions are my own.
https://www2.mrc-lmb.cam.ac.uk/group-leaders/n-to-s/lori-passmore/
biophysicist @ucsdbiosciences @hhminews, cell exploration, molecular anthropology
