I tried that but what worked the best was to germinate on agar germinating medium, then resuspend them gently and pipette them on grid. Not trivial but doable.
30.07.2025 04:14 — 👍 0 🔁 0 💬 0 📌 0
Congratulations!
29.07.2025 20:05 — 👍 2 🔁 0 💬 1 📌 0
New preprint: In situ Architecture of #Plasmodesmata.
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
26.07.2025 15:38 — 👍 192 🔁 71 💬 9 📌 8
Our methods paper made it to bioRxiv:
doi.org/10.1101/2025...
Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM
19.02.2025 06:06 — 👍 118 🔁 43 💬 2 📌 5
Great news ! Will give it a look. The tomograms look very nice!
I will release the data on the CZI public tomography portal
29.07.2025 20:03 — 👍 1 🔁 0 💬 1 📌 0
'cux good data don't wait!
24.07.2025 16:11 — 👍 2 🔁 0 💬 0 📌 0
Outstanding work from Christine, Stan and the whole team on this beautiful story. Wow.
17.07.2025 13:44 — 👍 5 🔁 1 💬 0 📌 0
Reminds me of the South Park episode where Randy tries to do the biggest poop in the world
16.07.2025 23:06 — 👍 0 🔁 0 💬 1 📌 0
C'est a Arcachon ça ? Sur la promenade ? J'ai aussi cru a une vrai baleine. Mais vu les couleurs quand je me suis rapproché....
16.07.2025 21:18 — 👍 1 🔁 0 💬 0 📌 0
Big incel power tripping vibs right here. Dangerous when those kinds of people hold that kind of power.
14.07.2025 06:40 — 👍 0 🔁 0 💬 0 📌 0
Yup. Have been saying this since Trump started overstepping. Where are all the wannabe heroes doing CQB training on weekends to prepare against the evil government? Prob no where because they are on the good side of the fence...for now...
13.07.2025 06:21 — 👍 2 🔁 0 💬 0 📌 0
When I was at UCLA, I started a similar project on hex1 bio crystals in fungus to solve by fib milling microED. Code name of the project was also IncelED 😂 odd coincidence!!
13.07.2025 03:44 — 👍 1 🔁 0 💬 0 📌 0
The maturity of a 5yo basically. Arrested development?
13.07.2025 03:34 — 👍 0 🔁 0 💬 0 📌 0
They are paid crap salary to do gestapo work too. They must really love the hunt.
10.07.2025 04:57 — 👍 3 🔁 0 💬 2 📌 0
Neural net picker with a truly general model - no per dataset training required (at least if it works as advertised). Want to try this on some messy/mixed samples where other approaches struggle!
09.07.2025 13:03 — 👍 6 🔁 2 💬 1 📌 0
Walkthroughs on how to apply protein design to cryo-EM (if you have experience in doing so). Please 🙏
30.06.2025 23:17 — 👍 1 🔁 0 💬 1 📌 0
Running Phenix and stuck?
Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program.
Big thanks to Tom Willinger for making this happen!
Try it out: phenix-online.org/version_docs...
23.06.2025 05:14 — 👍 26 🔁 10 💬 1 📌 0
Frostbyte 🥶 socks provided by @hammerspace.com
16.06.2025 15:48 — 👍 2 🔁 0 💬 0 📌 0
It was really fun! Do you mind linking to where all your python scripts for cryoEM are ?
13.06.2025 00:32 — 👍 0 🔁 0 💬 1 📌 0
And it’s done! Thanks to everyone who came out for the first edition of FrostByte, our event for the local computational cryo-EMers in the Bay Area ❄️
Special thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing 🙏
12.06.2025 01:44 — 👍 23 🔁 2 💬 1 📌 0
Sure does
10.06.2025 20:05 — 👍 1 🔁 0 💬 0 📌 0
Nice tattoo! I see a-helices? 😆
10.06.2025 00:11 — 👍 0 🔁 0 💬 0 📌 0
This paper combines two sort-of-crazy (in the best sense) approaches in cryoEM sample preparation:
(1) Native, soft-landing electrospray ion beam deposition, followed by (2) deposition of amorphous ice, and reheating and freezing by laser flash melting. Has potential for time-resolved studies.
09.06.2025 06:42 — 👍 36 🔁 12 💬 0 📌 0
09.06.2025 17:52 — 👍 21359 🔁 3773 💬 240 📌 156
Yet all the people who live to bear arms probably see no problem with this interference. As long as no one forces their kids to be trans 🤣
08.06.2025 06:02 — 👍 0 🔁 0 💬 0 📌 0
It's hype. They are just surfing on it and getting VC money by claiming that they will achieve this. Obviously they won't.
06.06.2025 11:10 — 👍 0 🔁 0 💬 0 📌 0
Doxing someone. Great job. You suck.
05.06.2025 16:37 — 👍 0 🔁 0 💬 1 📌 0
Your bar is very low if for you the cybertruck is doing well because it's driveable. It's not allowed in Europe because the design is dangerous and the sales are very very low. It fails at towing because of huge design flaws in the rear hitch and it's briques out if out of battery. Lmao
30.05.2025 21:47 — 👍 14 🔁 0 💬 1 📌 0
Improving accessibility of TEM and cryo-TEM in research and industry.
Postdoc at IGFL, ENS-Lyon.
Ecology-Evolution-Development
Nature Photography
website: https://arnaudbadiane.com/
CryoEM, membrane proteins and whatnot
An expedition to study coastal ecosystems and their response to the environment, from molecules to communities
Facts & strategy, in an authoritarian takeover.
Rightwing billionaires want to privatize NIH and use it to control universities.
We work to cure diseases like cancer.
Pers views. #science #medicine
European Research Council, set up by the EU, funds top researchers of any nationality, helping them pursue great ideas at the frontiers of knowledge. #HorizonEU
Cryo-electron tomography and subtomogram averaging. Pushing buttons, turning knobs, and furiously smashing keyboard. Assistant prof at Vanderbilt University; I ask other people to pipette things, often viruses.
Staff Research Associate @ UC Davis
🌱🍇🧪🧬🖥️ #PlantScience #Genomics
Grape (pan)genomics
Scripps Research Professor. HHMI Investigator. Nobel Prize Physiology or Medicine 2021. Opinions my own.
CNRS Research Director in Bordeaux 🔬 Structural biologist & microbiologist, father of 2, daily cyclist (#velotaf), runner (just for fun). mountain & nature enthusiast.
UCL/Birkbeck and institut Pasteur alumni
fronzeslab.cnrs.fr
#cryoEM #cryoET #microsky
cellarchlab.com Univ. of Basel Biozentrum🇨🇭. Exploring molecular architecture inside cells with #CryoEM #TeamTomo. Plants and algae in a changing climate. ❄🔬 OF 🌿 4THE 🌍!
Joint Head of Structural Studies at @mrclmb.bsky.social. Develops & uses #cryoEM to study amyloids in neurodegeneration. #tau, #alphasynuclein, #opensoftware, #RELION. All opinions my own.
Scientist, 2017 Chemistry Nobel Prize (math and computational methods underlying cryo-EM of biological molecules), fiction writer, photographer, gardener.
Climate change, science education, fight of fascism
franxfiction.com and joachimfranklab.org
Assistant Professor at Utrecht University 🇳🇱
We use #cryoEM and molecular #virology techniques to study the infection mechanisms of +ssRNA viruses.
https://www.uu.nl/medewerkers/DLHurdiss
Views are my own.
Molecular biologist. Group leader at MRC LMB Cambridge UK. Fellow at Clare Hall college. #CryoEM #RNAbiology #DNArepair
Views/opinions are my own.
https://www2.mrc-lmb.cam.ac.uk/group-leaders/n-to-s/lori-passmore/
biophysicist @ucsdbiosciences @hhminews, cell exploration, molecular anthropology
