Seeger Lab

Laser flash melting cryo-EM samples to overcome preferred orientation - Nature Methods Individual proteins tend to adopt preferred orientations when subjected to vitrification for cryo-electron microscopy analysis. A laser flash melting procedure followed by rapid revitrification provid...

A laser flash melting procedure, followed by rapid revitrification, provides a simple approach to help mitigate the preferred orientation problem that plagues cryo-EM analysis.

www.nature.com/articles/s41...

We are hiring a Postdoc in membrane protein cryo-EM at the University of Georgia. Looking for candidates with expertise in membrane protein purification and biochemistry. Fully funded for up to 5 years. Join us in Athens, GA. Apply: camilo.perez@uga.edu

always a pleasure discussing all things #tuberculosis with this group @seegerlab.bsky.social @berndwollscheid.bsky.social & Michael Berney

Dear all,

I am very excited about the upcoming 842 WE-Heraeus-Seminar #Cryo-EM on the Move. @djanuliene.bsky.social and I are looking forward to a great conference with many exciting speakers!

We had an amazing time with the ABC family and already miss you all! Can’t wait to hear your exciting talks, have great discussions, and hit the slopes of the Alps again. #ABC2025

See you next time!🗻⛷️🚠🧬🧫🧪💙

Proposed mechanism for import and reduction of mycobactin by IrtAB

Structural biology:

#Mycobacteria use molecules called mycobactins to capture iron, then taken up by a membrane transporter

Gonda et al. show that mycobactin slides down a crevice at the transporter surface & goes across the membrane in a “credit card” mechanism

🦠🧪
www.nature.com/articles/s41...

Markus from @seegerlab.bsky.social wakes us up with a fresh take on metal metabolism in Mycobacteria... So you think you have an exporter on your hands? Make sure it doesn't import instead - no iron(y) 😁

Here you'll find everything a structure-function aficionado might hope for:
✅ Crystallography 💎
✅ Cryo-EM 🔬
✅ Nanobodies 🦙
✅ Mutational analysis 🧬
✅ Molecular dynamics simulations 🖥️

Congrats to Dr. Sille Remm for winning the 1st special prize from the President of Estonian Academy of Science "for the most elegant work" in the Estonian National Student Science Competition!🥳👏 So proud of you!

Check out this nicely written News & Views article by Parjit Kaur about our paper!

nature.com/articles/s4158…

Many thanks to our collaborators @Barandunlab, @kai_ehrenbolger for their great effort to determine the cryoEM structure of EfrCD💪💪☺️

Proud to see our DMS paper out in @nchembio after going through a productive review process🥳EfrCD's drug efflux activity profile determined for >1400 single variants. Identified single mutations that convert the exporter into a drug-specific ABC importer!

nature.com/articles/s4158…

For their project - IBD NanoBiotics - the team engineered gut microbiota member E. coli Nissle 1917 to secrete anti-inflammatory nanobodies which dampen inflammation in GI tract of IBD patients. Supervised by Kenny from @MartinJinek lab, Jenna and @f_ackle from our lab

Congrats to the @uzh iGEM team for winning the best therapeutics project🥇 and best new basic part awards🥇 and for getting into the top 10 among the 350 projects at this year's @iGEM Jamboree in Paris! We're happy for all your achievements! 🤩👏It was fun to host you in our lab!

new group photo... PARKOUR!🤸🏻‍♀️💨

😍😍

x.com/krparey/status…

The climax of a 5 year collaborative project with the @enricabordignon lab: spin-labelled nanobodies to probe the conformational space of MsbA in intact cells published in @ScienceAdvances. Structures by @MoellerGroup and MD simulations by Lars Schäfer lab

science.org/doi/10.1126/sc…

Congrats to Sille Remm for successfully defending her PhD thesis!🥳👩‍🎓 You did very well biting through this tough project! We're so proud of you!☺️☺️

The temperature dropped by a few degrees yesterday, so we decided to have a raclette on our rooftop🧀😋

Congrats to everyone! We are super proud of you, Imre 🤩💪@Prolatium

x.com/ITTS_Cure/stat…

Four weeks of time flied so fast! We're happy that you enjoyed your stay and we had a great time with you too. Many thanks for all the scientific discussions and after work beers🍻😉. We're excited to see the sybody selection platform being implemented in the @Intein lab🥳

t.co/x5vKO8BMQu

...and here are the heros of the review, Michèle Sorgenfrei and Lea Huber-Hürlimann. The PI is very proud!!

We're happy to announce that our latest review about biomolecules capturing live bacteria from clinical samples is out on @TrendsBiochem !!🥳🥳

doi.org/10.1016/j.tibs…

How many memes does it take for people to clean up?

4 unsuccessful attempts in form of memes to encourage people to clean their mess😅😅 #TGIF

On a special mission to join a delegation of the Round Table Antibiotics to talk with members of the Swiss parliament about the urgent need to increase the federal budget to fight #AMR 💊🦠

📢Our rapid response to the COVID-19 pandemic finally in peer-reviewed form @emboreports in collaboration with Philippe Plattet, @CPaulino_ @MembraneEnzymo1 🥳
„Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance“

doi.org/10.15252/embr.…

Preparing E.coli produced avocado smoothie🥑 for the afternoon apéro... or is it GFP-tagged protein?😜

We know it😅 thanks for measuring the samples!

x.com/enricabordigno…