Sam Collie

that being said i think it’s very rarely necessary.

caveat that all my experience is with magnetic dynabeads not sepharose.

pre-clearing lysates help a lot when your lysates are particularly sticky (if your medium is dense or there or in nuclear fractions with chromatin present). tradeoff is that your are depleting your sample but hopefully mostly of non-specific interactions.

So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵

Intracellular competition shapes plasmid population dynamics From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating g...

Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...

Success. Nearly 259 Foldscope deployed in hands of unsuspecting candy crazy kids. Halloween is always more colorful with the microscopes - specially in a few weeks they could look at the micro-fauna of the teeth with so much sugar consumption.

🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky

www.cell.com/cell/fulltex...

Great example of incredibly useful and relevant “negative” data

Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models Acetaminophen (APAP) is the most widely used analgesic during pregnancy, yet epidemiological studies have reported conflicting associations between prenatal exposure and neurodevelopmental disorders (...

I freaking love that this team must have been like TIME TO PREPRINT

Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models

www.biorxiv.org/content/10.1...

It’s fortunate that preprint servers are already well-established and robust. As an early career researcher, I still find the pull to publish as prestigiously as possible for my first paper. Recently I’ve started the painful process of disabusing myself of this notion.

With recent NIH news it seems much more plausible that the demise of Journals as the de facto method of sharing science is eminent.

How to be a scientist in a post-journal world When we created this site to write about things in science that need to be fixed, we chose not to start with publishing, even though it has been our preoccupation for decades.

This article by @pracheeac.bsky.social and @mbeisen.bsky.social sums up (in much stronger language) a lot of what I heard in a recent talk by Gary Ward (U of Vmt).

thescientistpapers.substack.com/p/how-to-be-...

Really compelling argument to get rid of p-values in cell biology in favor of confidence intervals. Everytime I think about the state of statistics in biological research it makes me want to run, hide and become a statistician. rupress.org/jcb/article/...

I'm not right wing but some of this "woke" stuff is a little over the top [6 months later] Slavery was fine. Your city is under martial law

Opinion | Don't get angry at ICE agents. They are just following orders.

by Adolf Eichmann

In vivo generation of CAR macrophages via the enucleated mesenchymal stem cell delivery system for glioblastoma therapy | PNAS Glioblastoma multiforme (GBM) is one of the most aggressive intracranial tumors for which there is no effective treatment. Chimeric antigen recepto...

Cool new method for generating CAR-Macrophages In Vivo utilizing their phagocytosis of exogenous ennucleated stem cells!
www.pnas.org/doi/10.1073/...

Ohio State announces every student will use AI in class COLUMBUS, Ohio (WCMH) — Starting this fall, every Ohio State student will be asked to use artificial intelligence. “Through AI Fluency, Ohio State students will be ‘bilingual’ — fluent …

"Through AI Fluency, Ohio State students will be ‘bilingual’ — fluent in both their major field of study and the application of AI in that area"

Ohio State really not beating the allegations out of Ann Arbor here.

Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation - Nature Cell Biology Arya et al. report that migrating neutrophils resolve acute inflammation by releasing exosomes associated with nuclear DNA. This process is distinct from the release of neutrophil extracellular traps,...

Excited to share our recently published study on how DNA secretion in neutrophils modulates their inflammatory response spearheaded by @sbarya.bsky.social

Thanks to all our collaborators and contributors for their help getting this story to press.

👏👏

www.nature.com/articles/s41...

Fascinating study with absolutely mind-blowing good EM images. I’m curious about these trimmer and junction points that form in the HFs. How stable are these structures? Is there a unique lipid composition required for this?

Also, is the idea that this is limited to HF structures or canon MVBs too?

Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu

#cryoET #trafficking #organelle #hemifusion

once admirable? our government has always existed to promote the interests of american corporations abroad, at any cost

Tissue-like multicellular development triggered by mechanical compression in archaea The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...

Pretty much everything is fucked, but here is some beautiful and exciting science from @archaeon-alex.bsky.social and colleagues.

www.science.org/doi/10.1126/...

Many brilliant scientists are now being punished by having our funding terminated because we are from underrepresented groups. We did everything right and we EARNED these awards. Remember us when you’re making decisions on whether DEI should be sacrificed to “protect” research funding. #InMyFeelings

Congratulations Tim!

Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores - Nature Communications The contribution of central and peripheral channels of nuclear pores to transport of transmembrane proteins is unclear. Here the authors show that most inner nuclear membrane proteins use only periphe...

This adds to another fascinating paper showing that membrane proteins like lamin B receptor travel not through the central channel at all, but around the side of the nuclear pore complex through a peripheral channel.

www.nature.com/articles/s41...

Overlapping nuclear import and export paths unveiled by two-colour MINFLUX - Nature High spatiotemporal precision tracking using 3D MINFLUX shows that nuclear import and export occur in overlapping regions of the central pore, providing insight into transport across the nuclear pore ...

Incredible findings regarding the mechanism of active transport through Nuclear Pore Complexes. Seems importin travels only through the periphery of the central channel leaving a large dead zone in the center.
www.nature.com/articles/s41...

Ångström-resolution fluorescence microscopy - Nature The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...

www.nature.com/articles/s41...
I am amazed at this achievement. This is done using fairly off the shelf components. 🧪

Thank you so much to our donors so far! You’re helping to make DFB possible. There’s still time to donate! You can use the link in our bio to access the official DFB Giving Blueday page. #givingblueday

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You can support us at maizeraise.umich.edu/schools/Univ...

Hear from current and previous instructors, some of our alumni and one of our faculty advisors about the importance of the work we are doing! Donate now to help support our mission.

love this game, it’s a slot machine with extremely good gameplay. still a lot of kinks to be worked out before release but the devs have been trying stuff out and it’s been improving significantly since the start of the beta