Tatiana Moiseeva

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

For now our university thinks it may be applied to all H1B holders at border crossing, they advise either to enter the US today before midnight, or stay put and not try

Oh, certainly not look kind. Just to iterate the fake "gold standard science" or something. They would still target specific universities, of course, but maybe generally all of them. I know, it is a weak argument, that's all i got

All I can do is hope at this time, there's not much else. Maybe giving universities a break could be a small performative gesture for them, it is such a tiny fraction of H1Bs🙈

My new senior scientist's H1B was approved yesterday, but now they can't come in. I am still hoping universities will be exempt🙈

Last evening President Trump issued a Proclamation titled Restriction on Entry of Certain Nonimmigrant Workers, which aims to block anyone seeking to enter the U.S. on an H-1B visa, unless a $100,000 fee has been paid by their employer. The best advice we can give at this time comes from outside counsel, Fragomen, Del Rey, Bernsen & Loewy, LLP. At this time, we recommend the following: (1) If you are currently in the United States in H-1B status, do not travel out of the country (even to Canada to Mexico) until further notice; (2) If you are currently outside of the United States with an H-1B visa, do everything you can to return before midnight ET tonight (Saturday), if possible; and (3) If you are outside of the United States and cannot get back by midnight ET tonight (Saturday), do not attempt to return until we know more about how the Order will be implemented.

When your incoming lab member's H1B was approved yesterday, but now they can't travel to the US...

a photo of me chatting with very nice other conference attendies, a glass in my hand, but my facial expression is very skeptical for some reason

Thanks CSHL for the photos, my facial expressions on them are priceless. I think this one is a keeper for replying to a lot of stuff on the internets...

absolutely, adding now!

A landscape showing green trees and some leaves changing colors, harbor water is seen down between the trees

Excited to be back at the CSHL meeting on DNA replication this week! Campus views are amazing as always, looking forward to all the science!

A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells Nature Communications - Determining how replication forks move across the human genome is critical for the effective use of agents that target replication stress. Here, the authors present...

Checkout our latest research in @natcomms.nature.com
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team

Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination Nature Communications - The mediator protein Rad52 promotes Rad51 binding onto RPA-coated DNA to initiate homologous recombination. Here, the authors show that Rad52 sorts Rad51 into monomers and...

How are Rad51 filaments formed during Homologous Recombination?

Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.

urldefense.com/v3/__https:/...

Thank you!!

From cooperation to conflicts – a complicated relationship between transcription and replication. A tight coordination between transcription and DNA replication is essential to maintain genome stability. Both excessive transcription and upregulated…

Our new review on how the initiation of DNA replication is coordinated with transcription is now online! Congratulations to @syedshahidmusvi.bsky.social!
www.sciencedirect.com/science/arti...

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic

It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...

Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...

a smiling young woman with a bouquet of flowers and a book (her printed thesis) is standing in front of a green screen with "school of science" written on it

Sameera Vipat, the first PhD student from our lab, successfully defended her thesis in Tallinn yesterday! Congratulations to Sameera, and a huge thank you to @lmyler.bsky.social for being an opponent and a great scientific discussion!

Agarose gel with a size ladder on the left and some random bands. In the upper right corner there is a written text at an angle "biotin HA 6/6"

We carried an agarose/EtBr gel to the imager on a plastic box cover that was labeled with red sharpie. The gel acquired a nice but unexpected label. How did this happen? The box was not imaged. Sharpies don't get washed off that easily. What kind of sorcery is this?
The label says "biotin HA 6/6"

How many trainees were supported by your award? Enter your answer 14.What was the reason given for your grant’s cancelation? Enter your answer 15.Is there anything else you would like to share with the Committee? Enter your answer 16.Are you comfortable with the Committee publicly sharing the information you provided? If you select “ask me first,” please be sure you have provided an email address or phone number. Yes No Ask me first

🧪 Attention those with terminated grants, including for NSF, NASA, EPA, NIH, etc:

Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela...

Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).

Excited to share our latest preprint by @mihyun-kim.bsky.social. Our works adds another piece to the puzzle how DNA incision activities in DNA repair pathways are regulated to ensure genomic stability. https://www.biorxiv.org/content/10.1101/2025.05.20.655039v1

Excited to share our latest preprint by @mihyun-kim.bsky.social showing how DNA incision activities in nucleotide excision are regulated to ensure genomic stability. We show that interactions between TFIIH and XPG control the 5' incision by ERCC1-XPF. www.biorxiv.org/content/10.1...

Multigenerational cell tracking of DNA replication and heritable DNA damage - Nature A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early even...

Delighted to share that the results of my main postdoc project are now published in @nature.com

🔗 doi.org/10.1038/s415...

Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...

Hey everyone! Every year our HIllman Cancer Center organizes a fundraising bike ride to raise money for cancer research. Sad, but currently it is especially relevant. If you like this cause, please consider supporting our Genome Stability team!
fundraisers.rushtocrushcancer.org/tatiana-mois...

A huge thank you to our speakers @kajastelab.bsky.social and Raphael Ceccaldi for their amazing talks and fascinating research at yesterday's webinar!
Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social.
Join our community: docs.google.com/forms/d/e/1F...

24th Annual Midwest DNA Repair Symposium The 24th Annual Midwest DNA Repair Symposium will be held at the University of Michigan in the Kahn Auditorium of the Biomedical Science Research Building (BSRB) on May 17–18, 2025. The event will sta...

24th Annual Midwest DNA Repair Symposium
University of Michigan
May 17-18, 2025, Ann Arbor

sites.google.com/umich.edu/um...

"Five judges ruled that the U.K. Equality Act means trans women can be excluded from some groups and single-sex spaces such as changing rooms, homeless shelters, swimming areas and medical or counseling services provided only to women." This has nothing to do with biology.

🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome

Happy to post our first preprint in a while! www.biorxiv.org/content/10.1.... This was a slightly new direction for us, spearheaded by amazing post-doc @audreylumeau.bsky.social. Short thread to follow 🧬

Absolutely, adding you now! You can find the starter packs in the pinned post on my page. Welcome to bluesky:)

Genome rearrangement diagram with segments in different colors, connected by thin lines

The cover reminds me of this type of genome rearrangement diagrams. Not sure if this is intentional, but it looks very nice, congratulations!

a woman is covering her mouth with her hand while holding an arrow in her hand . Alt: a woman is covering her mouth with her hand while holding an arrow in her hand .

NIGMS Advisory Council for May 22 has finally posted to the Federal Register. May the odds be ever in your favor, friends.

www.federalregister.gov/documents/20...