Samuel Pazicky

You could do on-bead digestion to exclude the SDS. For bioID I used to do on-bead digestion and then 1st “elution” with AmBic and 2nd with 4:1 ACN:TFA. As for removal of SDS, I would be worried to go for anything less than SP3. Still weird that it’s only a particular treatment though!

06.04.2025 00:37 — 👍 1    🔁 0    💬 1    📌 0

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22.11.2024 09:02 — 👍 41    🔁 32    💬 0    📌 0

Just a reminder that Germany was the center of scientific progress until the Nazis suppressed, drove away, or killed all the non-Nazi scientists.

13.02.2025 18:54 — 👍 3836    🔁 1057    💬 64    📌 34

Instead of valentines this year, I’m sending copies of this tiny little book to family and friends. If you have not read it, get two copies today— one to keep and one to give.

The first lesson: Do not obey in advance.
The second: Defend institutions.

It could not be more relevant.

08.02.2025 23:12 — 👍 54    🔁 18    💬 3    📌 1

Thank you, yes I was reading this paper just few days back. Our experience with FAIMS/DDA is already quite different than theirs (no boost in protein IDs but more unique peptides with FAIMS in my hands), so I assume FAIMS/DIA might also end up being different...

23.01.2025 03:30 — 👍 0    🔁 0    💬 0    📌 0

Thank you, this is what I was thinking too. Definitely will try library-free as well and stick with what works better.

23.01.2025 03:21 — 👍 0    🔁 0    💬 0    📌 0

I am planning to switch from DDA to #DIA for our proteomics experiment on Orbitrap Exploris. Question: FAIMS yes or no? If I do not use FAIMS for library generation, can I use it for DIA measurements? Or the other way around? #massspectrometry #proteomics #teammassspec

21.01.2025 05:16 — 👍 3    🔁 2    💬 4    📌 0

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Thank you so much to whoever put this very comprehensive list of #science Bsky starter packs together. This is just amazing: docs.google.com/document/d/1...

26.11.2024 02:47 — 👍 564    🔁 313    💬 49    📌 37