Fraley Lab

@shuvoroy.bsky.social @pannierlab.bsky.social @mokhalil.bsky.social @hollister-lab.bsky.social @helenhuang.bsky.social @shellakeilholz.bsky.social @josh-leonard.bsky.social @gudalab.bsky.social @thechunglab.bsky.social @fischbachlab.bsky.social @fraleylab.bsky.social @staceyfinley.bsky.social

This has implications for regenerative medicine, where degradable scaffolds are used to promote cell infiltration and tissue repair. #science #biomaterials @ucsandiego.bsky.social

Since biomaterial properties are typically assessed post-manufacture without accounting for cellular modification, adjusting for degradation effects will be crucial for engineering biomaterials that sustain desired environmental conditions and regulate cell behavior effectively.

It also shows that local degradation by cells can result in materials that are biophysically distinct from their initial characterizations.

This study shows that hydrogel degradability can be used as a design variable to engineer stress relaxation and cellular adhesion, with far-reaching implications for the development of future biomaterials.

Cells embedded inside these synthetic hydrogels and using their own MMPs showed a similar response, spreading less in the cell-degradable (CD) gels than the non-degradable (ND) gels.

Cells sensed these MMP-induced changes in stress relaxation, spreading less and forming fewer and smaller focal adhesions.

To verify that cells were actually sensing the changes in stress relaxation caused by MMPs, we engineered a synthetic hydrogel with MMP-degradable peptides. When treated with increasing amounts of MMPs, its stiffness doesn't change, but it relaxes faster, like the natural ECM.

Cells sensed the MMP treated ECM, spreading less that they normally do on the untreated ECM.

and the mechanics of the ECM. Increasing MMPs didn't change the stiffness of the ECM, but did increase its stress relaxing behavior.

In this study, the incredibly talented @badznithin.bsky.social asked whether the small cuts in the ECM cells make with their MMPs change the mechanics of the environment in a way that cells can feel...a feedback loop! We found that even small amounts of MMPs change the architecture...

edward scissorhands is holding a pair of scissors in his hand ALT: edward scissorhands is holding a pair of scissors in his hand

Cells do this in multiple ways, including by using enzymes called matrix metalloproteinases (MMPs) to trim away at their environment, the extracellular matrix (ECM).

📢Paper alert: Matrix degradation enhances stress relaxation, regulating cell adhesion and spreading | www.pnas.org/doi/10.1073/... 🧵Often our studies focus on how the mechanics of the environment impact cell behavior...but it's a two-way street...cells can also effect changes on their environment...

This has implications for regenerative medicine, where degradable scaffolds are used to promote cell infiltration and tissue repair.

Since biomaterial properties are typically assessed post-manufacture without accounting for cellular modification, adjusting for degradation effects will be crucial for engineering biomaterials that sustain desired environmental conditions and regulate cell behavior effectively.

It also shows that local degradation by cells can result in materials that are biophysically distinct from their initial characterizations.

This study shows that hydrogel degradability can be used as a design variable to engineer stress relaxation and cellular adhesion, with far-reaching implications for the development of future biomaterials.

Cells embedded inside these synthetic hydrogels and using their own MMPs showed a similar response, spreading less in the cell-degradable (CD) gels than the non-degradable (ND) gels.

Cells sensed these MMP-induced changes in stress relaxation, spreading less and forming fewer and smaller focal adhesions.

To verify that cells were actually sensing the changes in stress relaxation caused by MMPs, we engineered a synthetic hydrogel with MMP-degradable peptides. When treated with increasing amounts of MMPs, its stiffness doesn't change, but it relaxes faster, like the natural ECM.

Cells sensed the MMP treated ECM, spreading less that they normally do on the untreated ECM.

and the mechanics of the ECM. Increasing MMPs didn't change the stiffness of the ECM, but did increase its stress relaxing behavior.

In this study, the incredibly talented @badznithin.bsky.social asked whether the small cuts in the ECM cells make with their MMPs change the mechanics of the environment in a way that cells can feel...a feedback loop! We found that even small amounts of MMPs change the architecture...

Cells do this in multiple ways, including by using enzymes called matrix metalloproteinases (MMPs) to trim away at their environment, the extracellular matrix (ECM).

Thank you @bmes-cmbe.bsky.social for the gala dinner last night to celebrate the CMBE Rising Star Awardees! ⭐️ Hoping more of these superstars join BlueSky! @marianhh.bsky.social @rituraman.bsky.social @fraleylab.bsky.social