Aydogan Lab

Looks very useful indeed! Thank you for sharing!

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

What if the legendary psychologist and grandfather of behavioral economics, Daniel Kahneman took a sabbitical in a fly lab? How would the lab culture change?

Explored this imaginary scenario in a blog post @the-node.bsky.social
thenode.biologists.com/the-kahneman...

Great read Sameer! Kahneman’s thinking fast and slow is the first book we recommend to our newly minted PhD students in the lab (System 1 vs 2 interpretation of the data) :))

It used to be a massive pain before every paper submission! xD

Figure 1: Fluorophore intensity in the Drosophila blastoderm (n.c. 14). Comparison of (A) green and (B) red fluorescence intensity using the same intensity scaling in n.c. 14. The fluorescence signal did not saturate. Shown are single imaging planes. Normalised histograms of fluorescence intensity for green (C) and red (D) fluorophores averaged across at least n=3 embryos per line. Scale bars = 20 μm

Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...

Cardiolipin dynamics promote membrane remodeling by mitochondrial OPA1 - Nature Communications This study reveals how cardiolipin governs mitochondrial morphology by modulating the activity of human OPA1 and how its replacement by monolyso-cardiolipin, as observed in Barth syndrome, impacts mitochondrial membrane-shaping mechanisms.

Excited to share that our paper on mitochondrial cardiolipin dynamics is published in @natcomms.nature.com today! We provide molecular explanations for unique aspects of mitochondrial morphology and the mechanisms underlying cardiolipin-related Barth Syndrome.
www.nature.com/articles/s41...

We are hiring an Assistant Professor of Biochemistry and Biophysics at Weill Cornell Medicine. Come join our collaborative research environment with state-of-the-art facilities in NYC! Application review starts Oct 22.
biochem.weill.cornell.edu/join-our-fac...

Towards a unified framework for the function of endoplasmic reticulum exit sites Nature Reviews Molecular Cell Biology, Published online: 29 September 2025; doi:10.1038/s41580-025-00899-0Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

New Online! Towards a unified framework for the function of endoplasmic reticulum exit sites

Dynamic cell fate plasticity and tissue reintegration drive functional adult synovial joint regeneration after complete resection - Nature Communications Joint injury and disease are leading causes of disability, with mammalian joints exhibiting poor regenerative capacity. Here the authors showed that after loss of a whole joint, adult zebrafish regenerate de novo articular cartilage, ligament, and synovium into a complex joint organ.

📣🧪 Read our latest #research on joint regeneration in #zebrafish, published today in @natcomms.nature.com www.nature.com/articles/s41... @columbiauniversity.bsky.social

🤩🤩🤩🤩

Are you excited?? Because I am!

25 years ago I was 10 and had just started secondary school yet our journal was already covering groundbreaking science, long before I'd even heard of molecular cell biology 🧐
Safe to say I'm fairly familiar with it now 🤭

Enjoy our issue!!!!!!!!!!!

This is absolutely a must read. We are in deep trouble and I am even less optimistic than the authors.

Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...

New cell cycle meeting starts next summer!
events.faseb.org/event/c40174...

Reductive evolution of the DNA replication machinery in endosymbiotic fungi The molecular machinery for replicating and repairing DNA accurately is critically important for life and highly conserved across the Tree of Life. Here we show that two major lineages of fungi, Glome...

I’m super happy to present the first discoveries of the RIKEN-Cambridge Joint Crop Symbiosis Research Team, based in Japan 🇯🇵

doi.org/10.1101/2025...

A thread 👇

David Baltimore, giant of molecular biology, has died.

🧵Does the nucleus set the cell cycle clock? 🕒

In frog egg extract “mini-cells” we see that as nuclei grow, cycles slow down. The period scales with the nuclear-to-cytoplasmic ratio, across Xenopus species, and even when DNA replication or transcription are blocked.

👉 doi.org/10.1016/j.cu...

Exciting preprint on organelle mechanoadaptation from Stephanie Miserey and Felix Campelo @felixmendu.bsky.social! Extracellular forces modulate Golgi secretory output by tuning carrier biogenesis via microtubule acetylation, DAG, and PKD. Golgi is a mechanoregulator!
www.biorxiv.org/content/10.1...

“‘She uncovered the social experience of women in science’ — the lab assistants who never became managers, the geologists poring over data in government offices while their male peers were doing fieldwork, those who despite their advanced degrees didn’t get hired or promoted, or who were sidelined…”

This is so thought-provoking…

ourworldindata.org/data-insight...

If you're finishing PhD soon and thinking of postdocs, see below!

Especially if you're keen on combining biochemistry, imaging and cell biology, we are onto some exciting findings on molecules and mechanisms underlying a cytoplasmic division cycle autonomously of CDKs!!

Postdoctoral Scientist - Aydogan Lab Primary Work Address: HHMI/Columbia University | Shadlen Lab--L5-003, New York, NY, 10027 Current HHMI Employees, click here to apply via your Workday account. The Howard Hughes Medical Institute (HHM...

#Postdoc-opportunity at Columbia U. in New York!!

Looking for a postdoc position? We are interested in candidates from biochemistry background & excited about fractionation, purification and reconstitution to study autonomous clocks in the cell cycle, in colab. with @aydinlab.bsky.social at NYU!

High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...

We are proud to be a founding contributor to lipidinteractome.org, a repository developed by @tafesselab.bsky.social & Schultz lab to increase accessibility to proteomics data from multi-functionalized lipid analogs! Check out the website & preprint: arxiv.org/abs/2507.23101 #lipidtime

Oh WOW - that's what we just needed 😍

Excited to share that I will be a co-director for the upcoming Workshop on Phylogenomics @evomics.bsky.social, alongside the amazing main team lead @rosafernandez.bsky.social and Erin K. Molloy

We are putting together an amazing workshop with details to come - can't wait to share it with everyone!🥳

Massive congrats Ishmail!!! We need to do celebratory coffee sometime soon when you have time!!

Funny that the virtual cell virtually encapsulate everything except....cell biology...(yes, there is a full world of complex behaviour outside the realm of gene expression) 🤷‍♂️

Thank you for your kind recognition, Jeremy!!