Alessandro Carrer

AI-rendered illustration of the article's title: Sucralose consumption ablates cancer immunotherapy response through microbiome disruption

Sucralose may interfere with cancer immunotherapy - exciting and important new research from @overacrelab.bsky.social in @theaacr.bsky.social!

aacrjournals.org/cancerdiscov...

(AI-rendered illustration of the article's title)

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2-hydroxyglutarate mediates whitening of brown adipocytes coupled to nuclear softening upon mitochondrial dysfunction - Nature Metabolism CLPP-deficiency-driven mitochondrial dysfunction in brown adipose tissue leads to the accumulation of d-2-hydroxyglutarate, in turn promoting lipid-droplet enlargement by altering gene expression and ...

Very happy to have contributed to this exiting story on nuclear softening caused by metabolic switch caused by oncometabolite 2-HG causing whitening of the brown adipocytes Trifunovic lab CECAD Cologne softeninghttps://www.nature.com/articles/s42255-025-01332-8?utm_source=dlvr.it&utm_medium=twitter

Congratulations Sara and team!!

Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff) | Epigenetics Podcast In this episode of the Epigenetics Podcast, we caught up with Steven Henikoff from the Fred Hutchinson Cancer Research Center in Seattle to talk about his work on Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC. In the last few years Steven Henikoff has been developing methods which profile the chromatin landscape by using enzyme tethering. The quest first started with ChEC-Seq, which improved on Uli Laemmli's method of Chromatin endogenous cleavage (ChEC) but used sequencing as a read-out rather than southern blotting. Next, Cleavage Under Targets & Release Using Nuclease (CUT&RUN) was developed by making a fusion protein of Protein A and micrococcal nuclease (MNase), making it possible to achieve antibody-targeted cleavage of chromatin fragments. And finally, Cleavage Under Targets & Tagmenation (CUT&Tag) was developed by using Transposase Tn5 instead of MNase, which adds sequencing adapters and fragments chromatin at the same time, streamlining the protocol even further. In this episode we discuss how working on centromeres set the stage for Steven Henikoff’s subsequent work, how he developed CUT&RUN and CUT&Tag, what the advantages and disadvantages of those methods are and how he developed all those experiments at home in his garage.   References Takehito Furuyama, Steven Henikoff (2009) Centromeric nucleosomes induce positive DNA supercoils (Cell) DOI: 10.1016/j.cell.2009.04.049 Kristina Krassovsky, Jorja G. Henikoff, Steven Henikoff (2012) Tripartite organization of centromeric chromatin in budding yeast (Proceedings of the National Academy of Sciences of the United States of America) DOI: 10.1073/pnas.1118898109 Jorja G. Henikoff, Jitendra Thakur, … Steven Henikoff (2015) A unique chromatin complex occupies young α-satellite arrays of human centromeres (Science Advances) DOI: 10.1126/sciadv.1400234 Epigenomics Methods: ChEC-Seq, CUT&RUN, AutoCUT&RUN, Improved CUT&RUN, CUT&Tag, Automated CUT&Tag, CUTAC, scCUT&Tag.   Related Episodes The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw) Hi-C and Three-Dimensional Genome Sequencing (Erez Lieberman Aiden) In Vivo Nucleosome Structure and Dynamics (Srinivas Ramachandran)   Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com

CUT&Tag and CUT&RUN: Redefining the Standards for Protein–DNA Mapping in Epigenetics: https://epigenetics-podcast.micro.blog/2025/07/30/cuttag-and-cutrun-redefining-the.html

Landmark

PEX39 facilitates the peroxisomal import of PTS2-containing proteins - Nature Cell Biology Chen et al. show that PEX39 cooperates with PEX7 in the peroxisomal import of proteins containing a PTS2 site and uncover an (R/K)PWE motif in PEX39 and PEX13 that binds to PEX7 and facilitates the im...

Our work on new paradigm in peroxisome biogenesis and PEX39 (1st human peroxisomal biogenesis protein (peroxin/PEX) found in > 20 yr) is out in @natcellbio.nature.com! Equal collab w/Tony Rodrigues (Jorge Azevedo lab), @dwendscheck.bsky.social (Bettina Warscheid lab)

www.nature.com/articles/s41...

HIF-independent oxygen sensing via KDM6A regulates ferroptosis Minikes et al. define an HIF-independent oxygen-sensing mechanism that regulates ferroptotic death through modulating the phospholipidome. When oxygen is limited, the histone demethylase KDM6A is inac...

HIF-independent oxygen sensing via KDM6A regulates ferroptosis: Molecular Cell www.cell.com/molecular-ce...

Debunked: Deepfakes of Brendan Gleeson, Luke O’Neill, and Sharon Tobin appear in hoax medicine ads The AI-generated celebs tout a miracle cure which they claim has been banned in Ireland.

This describes a scam video of me which seeks money. Please resend.

www.thejournal.ie/debunked-sca...

Distribution of the NCCN stages of #PancreaticCancer ?

16,412 pts (2017-2022) 🇳🇱
👉23% primary resectable
👉 8% borderline resectable
👉 11% locally advanced
👉 58% metastatic

⏩️ academic.oup.com/bjs/article/11…

S1PR1 in cells of lymphatic vessels regulates formation of vessel valves & abnormal lymph node-like structures called tertiary lymphoid organs

📷 Xin Geng et al @omrf.bsky.social in @jem.org

➡️ bpod.org.uk/archive/2025/7/8

Nutrients activate distinct patterns of small-intestinal enteric neurons - Nature Calcium imaging of the mouse jejunum shows that specific nutrients in the intestinal lumen are detected by epithelial cells, which activate specific sets of enteric neurons through a serotonin-mediate...

www.nature.com/articles/s41...

Nutrients activate distinct patterns of small-intestinal enteric neurons

Amazing short talk by #KonstantinosKolliopoulos at the 2025 iscam2025.org on how nutrient restriction decreases metastasis formation! @vib-ccb.bsky.social @vetenskapsradet.bsky.social

An unexpected career in cancer metabolism Nature Cancer - Ralph DeBerardinis obtained an MD and PhD from the University of Pennsylvania, then trained in Pediatrics and Medical Genetics at Children’s Hospital of Philadelphia. He moved...

Thanks Nature Cancer for the chance to reflect on some factors that led me to study cancer metabolism. For the trainees and other young scientists: the critical events that shape your career might not be obvious while they are happening. Keep an open mind and trust your instincts.

rdcu.be/evi4I

The European Pancreatic Club 2025 meeting in #Dusseldorf 🇩🇪 was a big success. EPC is the only #European wide multidisciplinary meeting regarding only #Pancreas #Pancreatitis #PancreaticCancer

⏩️Excellent #RCTs discussed: #CASSANDRA, #DIPLOMA1, #DIPLOMA2, #PANDRA2, #ESPAC6, #ESPAC7, #SIMBA

Uridine Uridine is a pyrimidine nucleoside composed of an uracil base and a ribose sugar. In cells, uridine is predominantly present in RNA (as uridine monophosphate, UMP). Uridine can be produced through de ...

Uridine: Trends in Endocrinology & Metabolism www.cell.com/trends/endoc...

a great not-to-miss piece from the @lyssiotislab.bsky.social

AKT-mediated phosphorylation of TSC2 controls stimulus- and tissue-specific mTORC1 signaling and organ growth Mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) integrates diverse growth signals to regulate cell and tissue growth. How the molecular mech…

www.sciencedirect.com/science/arti...

great work from @bdmanning.bsky.social

Now online in Cancer Discovery: Germline-Somatic Liaison Dictates Cancer Subtype via de novo Steroid Biosynthesis - by Paola Gasperini, Alessandro Alaimo, Blerta Stringa, Andrea Lunardi, Francesca Demichelis, and colleagues doi.org/10.1158/2159...

The Aging Microenvironment as a Determinant of Immune Exclusion and Metastatic Fate in Pancreatic Cancer Aging is a critical yet understudied determinant in pancreatic ductal adenocarcinoma (PDAC). Despite a strong epidemiological association with age, conventional PDAC preclinical models fail to capture...

New preprint! 🧓⚡️ Aging drives immune exclusion & metastasis in PDAC by rewiring the tumor stroma. Young CAFs ♻️ rejuvenate the aged microenvironment & restore immune infiltration.
Read: www.biorxiv.org/content/10.1...

#PDAC #Aging #TumorMicroenvironment #Preprint

🧵5 Top Free Alternatives to BioRender for Scientific Illustrations!

These five websites offer free scientific illustrations for biologists. Great for presentations, research papers and other research communication needs.

Save and share the post!

Cellular and organismal function of choline metabolism - Nature Metabolism

REVIEW | Kıvanç Birsoy, Monther Abu-Remaileh and colleagues @rockefelleruniv.bsky.social, @stanford.edu

The authors provide a broad overview of choline metabolism, its cellular handling, and how it impacts physiology and pathology.

https://bit.ly/3WPCPM2

Metabolic engineering to facilitate anti-tumor immunity While high fructose intake has been implicated in the progression of metabolic disease and cancer, Schild et al. take advantage of the availability of fructose in the tumor microenvironment. Engineere...

Schild et al. take advantage of the availability of fructose in the tumor microenvironment. Engineered immune cells can use metabolic flexibility and leverage fructose as a nutrient source to fuel their metabolism and facilitate function in vivo. www.cell.com/cancer-cell/...

What are the prognostic factors in patients with resected #PDAC that receive NAT? 💊🏥

#PANCPALS published last week in Annals of Surgery!

Key: prognostic factors for OS differ between chemo-naive and NAT patients.
Find the prognostic tool on our website soon! 💪
@ammaraj.bsky.social

I'm co-organizing a Cell Symposia on Metabolites in Organellar Communication with @robzonculab.bsky.social, Kivanc Birsoy, and Allyson Evans (@cp-cellmetabolism.bsky.social). It will be in Sitges May 11-13. Submit an abstract by Feb 14. Hope to see you in Spain! cell-symposia.com/metabolites-...

Schedule of speakers at the 2025 FASEB Ferroptosis conference

Registration is open for our summer FASEB #Ferroptosis Conference July 27 - 31, 2025. We have a terrific line up of speakers and it's going to be amazzzing! Come join us for this historic first meeting! Please help us to spread the word. 🤩
shorturl.at/QA6T1

On the International Day of Women and Girls in Science, we celebrate all female researchers who push the frontiers of knowledge!

Introducing @noeliaferruz.bsky.social - a chemist @crg.eu aiming to design a digital mind capable of explaining its reasoning.

👉 aiproteindesign.com

OPPORTUNITY TO JOIN US!

Two of these PhD positions are available in our group.

We want to know how nuclear metabolism impacts chromatin regulation in leukaemia.

You as well?
> Then check this out!

ICYMI by First Release - now officially in print @science.org

Specific combinations of nutrients and metabolic enzymes determine T cell differentiation via loci-specific histone acetylations.

https://buff.ly/4gvqKTz

From Shixin Ma, Sue Kaech @tcellogic.bsky.social et al.

#immunometabolism

GnP metabolism on the rise