Parijat Biswas

Announcing our new PA biosensor, PILS-Nir1 – seen here in HeLa cells during PLC activation #lipidtime #phospholipids

Read the OA paper in JCB

Non-muscle Myosin II acts as a negative feedback mediator to control cell contraction dynamics Local cell contraction dynamics play a crucial role in tissue and cell morphogenesis. Contractions near the cell edge drive highly dynamic cell shape changes during cell migration and contraction puls...

Non-muscle Myosin II acts as a negative feedback mediator to control cell contraction dynamics

www.biorxiv.org/content/10.1...

Kinesin-induced buckling reveals the limits of microtubule self-repair https://www.biorxiv.org/content/10.1101/2025.09.08.672697v1

Are these not chromosomes?

Localization of WDS proteins and fimbrin in three fungal species.

How do cells switch on the actin assembly at endocytic sites? How can this on-switch evolve? Check out our new preprint!
www.biorxiv.org/content/10.1...

How do cells link adhesion to protein synthesis? New findings suggest the protein p130Cas forms droplets that bud from focal adhesions and suppress translation, hinting at a novel mechanism for regulating cell state via phase separation.
buff.ly/2I99ZTc

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

Endothelial β-PIX (ARHGEF7) drives exocytosis through enabling the dynamic reorganisation of the cytoskeleton https://www.biorxiv.org/content/10.1101/2025.05.06.652370v1

DNA-nanoparticle motor.
www.nature.com/articles/s41...

Cartoon of techniques to apply one-dimensional, in-plane or volumetric cell compression in vitro.

In their Review, Laura Faure, Valeria Venturini @valeriaventurini.bsky.social and Pere Roca-Cusachs discuss the biological contexts of cell mechanical compression, the associated mechanisms and the experimental systems engineered to compress cells in vitro.
journals.biologists.com/jcs/article/...

Alpha-actinin-1 stabilizes focal adhesions to facilitate sarcomere assembly in cardiac myocytes https://www.biorxiv.org/content/10.1101/2025.03.28.645933v1

A coarse-grained model for simulations of phosphorylated disordered proteins https://www.biorxiv.org/content/10.1101/2025.03.19.644261v1

Are lipids actively sorted during clathrin mediated endocytosis like proteins? @mathilda95.bsky.social addresses this key open question together with our collaborators from the Honigmann and Modes labs using a new Lipid-STED workflow.
www.biorxiv.org/content/10.1...

Renganathan, Moor, @vgelfand.bsky.social et al. use single-particle tracking to show that #vimentin intermediate filaments display active movement throughout the cell, with individual filaments within a bundle moving independently rupress.org/jcb/article/...

#Cytoskeleton #Microscopy

CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration Cells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the...

As scientist I am going through difficult times (this is understatement of the year for me!!). Past one and half months have been horrible, however I am trying to pick myself up and super excited to share my postdoc work with the community! #cellmigration
www.biorxiv.org/content/10.1...

MINFLUX achieves molecular resolution with minimal photons - Nature Photonics The authors review MINFLUX super-resolution microscopy, outlining its advantages and limitations, recent progress, and an outlook for future developments.

An excellent review article of MINFLUX.
MINFLUX achieves molecular resolution with minimal photons
Lukas Scheiderer, Zach Marin & Jonas Ries
Nature Photonics, 19, 238–247 (2025)
www.nature.com/articles/s41...

This dot is a region that intersect multiple cell junctions in an epithelia. These dots act as tiny anchors of cell contacts and they’re critical for epithelial coherence during homeostasis AND development.

I’m @katecavanaugh.bsky.social and I’ll be your guide on this 🧵 about tricellular vertices.

Mechanics of Single Cytoskeletal Filaments - Annual Reviews just out from @romet-jegou-lab.bsky.social @cecileleduc.bsky.social @hugowioland.bsky.social:
www.annualreviews.org/content/jour...

Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair Phua et al. report that LIM proteins form force-dependent assemblies that bridge broken filament fragments. Zyxin assemblies coordinate F-actin nucleation by VASP and its crosslinking into aligned bun...

Jazzed to join the cool kids club and have our paper reporting the reconstitution of zyxin-mediated repair of contractile actin networks appear in @currentbiology.bsky.social ! bit.ly/41enkjo

A schematic picture of a cell, showing condensates, a nucleus and the cytoskeleton.

We wrote a review on the "Physics of droplet regulation in biological cells": arxiv.org/abs/2501.13639 Beside the basic #physics of phase separation, we discuss three aspects that separate cellular from traditional droplets:

Trypsin treatment improves Cellpose segmentation of brightfield images for adherent cells

Accessible and accurate cytometry analysis using fluorescence microscopes by Bo Huang and team:
www.biorxiv.org/content/10.1...

Excited that the peer-reviewed version of our fascin structure paper is now out in NSMB. Thanks very much to the referees for their constructive feedback. rdcu.be/d6Tct

Here, we create a genetically-encoded force probe by engineering a coiled-coil domain flanked by fluorescent proteins that undergo FRET. These probes are fully tunable and calibrated by modeling and single molecule force spectroscopy and allow one to measure forces within living systems.

Digital and Tunable Genetically Encoded Tension Sensors Based on Engineered Coiled-Coils
doi.org/10.1002/ange...

Stepwise order in protein complex assembly: approaches and emerging themes | Open Biology Protein-based nanomachines drive every cellular process. An explosion of high-resolution structures of multiprotein complexes has improved our understanding of what these machines look like and how th...

When a protein complex assembles in vivo, do subunits interact with each other in a specific step-wise order? This is something that few seem to think about, but we find it both important and fascinating. We wrote a review in Open Biology @royalsociety.org
royalsocietypublishing.org/doi/10.1098/...

Force transmission is a master regulator of mechanical cell competition Cell competition is a tissue surveillance mechanism for eliminating unwanted cells and as such is indispensable in development, infection and tumorigenesis. Although different biochemical mechanisms a...

Force transmission is a master regulator of mechanical cell competition www.biorxiv.org/content/10.1...

HT1080 cells with various morphologies. DAPI (blue) Phalloidin (grey) GM130 (green).

Advances in microscopy mean we can now do more than just observe biology—we can control it. But how far can we really push this in mammalian cells with all their (beautiful but annoying) heterogeneity? 🧪🔬(🧵)

#notthecover
Friction (rather than density of motors) guides actin network contraction. #resistance ✊
by @alexandra-colin.bsky.social
www.pnas.org/doi/10.1073/...

Vimentin-mSG FRAP

Well this is some cool single molecule imaging www.nature.com/articles/s41...

Thanks a lot!! I use the same Tubulin antibody, and I will try the vimentin one. Believe me, yours is the cleanest vimentin staining I have ever seen!!