Chetan Poudel's Avatar

Chetan Poudel

@biofizzycist.bsky.social

Assitant Professor of Medicine at Indiana University studying the kidney in 3D with lightsheet microscopy.

101 Followers  |  462 Following  |  8 Posts  |  Joined: 22.11.2024  |  1.9636

Latest posts by biofizzycist.bsky.social on Bluesky

Congrats to Adil Ali for getting his paper accepted at KI! He worked hard getting high-fidelity segmentation of all major structures of the kidney glomerulus, which was imaged at 100nm resolution! Check out a ton of interesting 3D data in the repository- including 3D printable files!

02.09.2025 19:44 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

We were already using #cellpose to segment kidney tubules but this combination with SAM should make it even more generalizable and powerful! Very exciting!

05.05.2025 02:32 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Imaging 3D cell cultures with optical microscopy Nature Methods - This Review discusses current 2D and 3D microscopy methods for imaging three-dimensional cell cultures and emerging strategies to address key challenges.

Our new review article on "Imaging 3D cell cultures with optical microscopy" is now out in @naturemethods.bsky.social. Congrats to Huai-Ching from Jon Liu's lab at UW for leading this effort! Open-access link here: rdcu.be/eh8g0

23.04.2025 17:57 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

Thanks Romain! Will be fun to build some lightsheet scopes and dive into computational analysis

22.04.2025 21:10 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Thrilled to announce that I will be joining Indiana University School of Medicine as an Assistant Professor this summer! A primary focus of my lab will be to develop technologies to study #kidney diseases in 3D at the organ-scale. Lots of fun imaging to follow!

22.04.2025 07:09 β€” πŸ‘ 4    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
Screen shot of the article

Screen shot of the article

01.03.2025 10:22 β€” πŸ‘ 192    πŸ” 78    πŸ’¬ 7    πŸ“Œ 6
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My annual Valentine’s post of this image that happens to land on #FluorescenceFriday this year! An axon approaching a kidney glomerulus doing its best to look like a rose.

14.02.2025 12:04 β€” πŸ‘ 269    πŸ” 36    πŸ’¬ 7    πŸ“Œ 2
Improvements in LM (top) and EM (bottom) of our micro-sam model (finetuned) compared to the default SAM model.

Improvements in LM (top) and EM (bottom) of our micro-sam model (finetuned) compared to the default SAM model.

After a long journey, Segment Anything for Microscopy is now published in Nature Methods! We significantly improve SAM for interactive and automatic segmentation in light and electron microscopy and build a user-friendly tool.
www.nature.com/articles/s41...

12.02.2025 11:41 β€” πŸ‘ 351    πŸ” 120    πŸ’¬ 11    πŸ“Œ 7
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an elderly woman is riding a roller coaster with her mouth open . ALT: an elderly woman is riding a roller coaster with her mouth open .

Live footage of the average NIH-funding academic as we cycle between maybe no longer having a job and court rulings allow NIH to resume evaluating and funding grants.
I do not recommend this life style, it sucks
Most of us just want to do important science unpeturbed.

12.02.2025 21:22 β€” πŸ‘ 734    πŸ” 89    πŸ’¬ 29    πŸ“Œ 7

That is very cool, I'll have to try that. I have so many videos on my ppts without source files

01.02.2025 01:36 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

Very cool! I wondered what the best way to transfer or move Zarr files on Windows is? Convert it to some single file zipped format?

28.01.2025 17:13 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Our work on multiplexed imaging using polymer dots made it to the cover of Science Advances! Out today and very timely for #fluorescencefriday

Paper here: www.science.org/doi/10.1126/...

13.12.2024 17:47 β€” πŸ‘ 5    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0

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