Dedecker Lab

Super-resolved Imaging of Molecular Interactions Using FRET-SOFI Super-resolution fluorescence microscopy is a powerful method to image molecular processes that occur beyond the diffraction limit of light. Förster resonance energy transfer (FRET) is widely used to ...

Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding Genetically-encoded fluorescent biosensors have revolutionized our understanding of complex systems by permitting the in situ observation of chemical activities. However, only a comparatively small se...

New preprint! Our work together with @agtebo.bsky.social : our 'NanoBlocks' strategy to generate fluorescent biosensors from binders such as nanobodies. Proof-of-concept sensors with ALFA nanobody & Erbin PDZ domain shows the concept in vitro, in cells, and for FACS.
www.biorxiv.org/cgi/content/...

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

We are at Innovative Imaging for 3D Cell Biology Conference in #Utrecht.
Come to talk to us! 😉

conference.imagine-microscopy.nl/programme

We are at Innovative Imaging for 3D Cell Biology Conference in #Utrecht.
Come to talk to us! 😉

conference.imagine-microscopy.nl/programme

We were happy to have Prof. Andriy Chmyrov (Helmholtz Zentrum München & NCT Dresden) as a guest speaker!
Thanks for joining us!

The setups will become part of the The Arenberg Optical Imaging Center (aoic.be) at KU Leuven.

It was 3D Fluorescence Imaging Inauguration Day today! 🥳

Together with the lab of Wim Vandenberg, we organized a launch event of two advanced custom-built 3D fluorescence microscopes! 🔬
🔺Triscan: by @robinvde.bsky.social (tinyurl.com/5t3r6x6k)
🔺Single-Objective Light-Sheet (SOLS) by Sergey Abakumov

🚨 Join us in shaping the future of #SmartMicroscopy! We're hiring PhD & fellowship positions at the intersection of #photonics, electronics & #AI.
Check out our works in this field
doi.org/10.1038/s414...
www.nature.com/articles/s41...
doi.org/10.1101/2024...
Please share!
#SPADarray #Microscopy

We are looking for a doctoral candidate to join the lab! If you are interested in working with human breast tissue samples straight from the clinic and do not fear large data sets, this might just be your dream project ☺️ Apply now ⬇️
ats.talentadore.com/apply/vaitos...
📸 @oonapaavolainen.bsky.social

Post-doctoral position in ultrafast laser opto-acoustics Université de Bordeaux

Call for a Post-Doctoral position

The I2M, Physical Acoustics Department is offering a position for a post doctoral researcher as part of an interdisciplinary project funded by @univbordeaux.bsky.social .

👉 www.u-bordeaux.fr/universite/t...

@gdr-imabio-ysn.bsky.social

It is my first Bluesky post: I am thrilled that our work on MemGraft probes for “permanent” fluorescent labelling of plasma membranes is finally out in JACS Au! We established a concept of lipid-directed covalent labeling of plasma membranes. Open access article:
pubs.acs.org/doi/10.1021/...

We are very happy that Solène Lefebvre was awarded #MSCA Postdoc Fellowship to work in our lab! 🥳🎉
Congratz, great to have you!

Results 2024 call: tinyurl.com/5n972ks4
#fellowship #noteasy #postdoc

PhD on high-throughput and super-resolution imaging of polymeric nanoparticles The TU/e is constantly looking for scientific and non-scientific staff further its ambitions. View here our current vacancies.

We have a PhD position open in our group! Interested in microscopy and want to help the design of better biomaterials for therapeutic applications? Come join us! Great collaboration with the Voets group at TU/e!

jobs.tue.nl/en/vacancy/p...

#MiFoBio2025

The autumn microscopy school by @gdrimabio.bsky.social will take place from 10th to 16th October 2025.

The call for workshops to share pratical knowledges is open till 31/01 ateliers-mifobio.fr

We'll bring stuff as usual, stay tuned !

More information 👉 linktr.ee/gdrimabio

For our annual Project Design Contest - postdoc, the winner is Solène Lefebvre with "STaRS = Smart Targeting and Release System" to improve drug delivery and development process.

Congratulations! 🥳🏆
#winner
#projectdesign

Super-resolution optical fluctuation imaging Nature Photonics - The authors present a review of super-resolution optical fluctuation imaging (SOFI), including its core working principle, recent advances and remaining challenges.

Although @ritastrack.bsky.social has publicly declared that Superresolution Optical Imaging (SOFI) is dead 🤓 I am happy to share our newest review about it rdcu.be/d52ak

Silke Denis is the winner of our annual Project Design Contest - PhD, with her “FPpace” project for fast continuous directed evolution of fluorescent proteins! 🏆
Congrats! 🥳
#winner
#projectdesign

Single molecule biology of bacterial cell wall remodelling at University of Warwick on FindAPhD.com PhD Project - Single molecule biology of bacterial cell wall remodelling at University of Warwick, listed on FindAPhD.com

Interested in a single molecule bacteriology PhD?
Deadline 16th Jan. Location Warwick, UK
Broad topic is the physical biology of the bacterial cell wall. Could suit almost any scientific background, eg biology, physics, biochemistry, chemistry, computer science.
www.findaphd.com/phds/project...

Promotional image for the 2025 RMS Early Career Award Competition. White text on purple background, QR code in bottom right, with opening date (9 Dec 2024) and closing date (14 Feb 2025) specified.

Applications are *OPEN* for our Early Career Award Competition 2025! @rmsearlycareer.bsky.social

Great opportunity to receive recognition for your work in #microscopy, #Imaging or #FlowCytometry

Shortlisted entrants will be invited to the final at #mmc2025

Enter now: rms.org.uk/opportunitie...

Big congratulations to our postdoc Robin for being awarded the Science and Innovation Award from the KU Leuven Faculty of Science! 🥳

Well-deserved, not only for work on the TriScan and the Circulator;)
@robinvde.bsky.social
#scienceaward

Picture courtesy: The Image Zone, Johan Van Droogenbroeck.

person in front of microscope and microscopy image

Curious what we do in my new lab at D-CHAB @ethzurich.bsky.social 🔬⚗️? Save the date for my inaugural lecture on April 3, 2025 and if you can’t wait to know more check out the news article and the associated video. chab.ethz.ch/en/news-and-...

Happy fluo Sinterklaas to everyone! 🙂🥳
mOrange, mScarlet and mCitrine in bacteria
... and mOranges to eat.

#fluorescence
#sinterklaas

High-Performance Chemigenetic Potassium Ion Indicator Potassium ion (K+) is the most abundant metal ion in cells and plays an indispensable role in practically all biological systems. Although there have been reports of both synthetic and genetically encoded fluorescent K+ indicators, there remains a need for an indicator that is genetically targetable, has high specificity for K+ versus Na+, and has a high fluorescent response in the red to far-red wavelength range. Here, we introduce a series of chemigenetic K+ indicators, designated as the HaloKbp1 series, based on the bacterial K+-binding protein (Kbp) inserted into HaloTag7 self-labeled with environmentally sensitive rhodamine derivatives. This series of high-performance indicators features high brightness in the red to far-red region, large intensiometric fluorescence changes, and a range of Kd values. We demonstrate that they are suitable for the detection of physiologically relevant K+ concentration changes such as those that result from the Ca2+-dependent activation of the BK potassium channel.

Ph.D. student Dazhou Cheng developed a chemigenetic K+ indicator (HaloKBP), using an rHCaMP/HaloCaMP-type design! pubs.acs.org/doi/10.1021/...

It is official! The fourth edidtion of our winter school on "Fluorescence markers for advanced microscopy" will take place on March 15-20 2026 in Les Houches, in the french Alps.
fluorescenceleshouches.wordpress.com
Selection of participants will start in 2025. Signup on the website to be informed.

🚨Recently, we also published the 'Resonator',
which provides quasi-simultaneous fluorescence imaging with multiple excitation wavelengths:
Check it out: 👇https://pubs.acs.org/doi/10.1021/acs.nanolett.4c00258

Very happy to share the 'Circulator' paper, which is now out in @naturemethods ! 🥳
👉https://rdcu.be/dTa4v
Together with @vandenberglab! https://twitter.com/naturemethods/status/1832095358425436636

Leuven Advanced Microscopy Symposium 2024 was great ! 🔬💫
Thanks to you !
#LAMS2024 @stiel_lab @VoschLab @vandenberglab @Bodeeen1 @JamesDManton @LazarLab @mueller_physics @wmarkbates @michellefrei13 @SusanSuperRes @Fluo_renzo @LReinkensmeier @huserlab @NielsRadmacher

We have several positions open in our lab ! 📢🥳
Check our tweet feed ! https://t.co/o8373NMxfF

And we have also a PhD (or potentially postdoc) position OPEN! 📢
Do you want to develop new biosensors for live-cell imaging ? 🦠🔬
Then join our team in the @DedeckerLab !

You can apply till 30/07/2024 ! 🤩👉
https://shorturl.at/KcTYO