Javi

Figure 1 from the preprint

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️

www.biorxiv.org/content/10.1...
#teamtomo

Text box says: "We are hiring".

Are you ready to lead ground-breaking research in Infection Biochemistry?

We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.

embl.wd103.myworkdayjobs.com/en-US/EMBL/j...

3D-Animation of the nanobody (light yellow/semi-transparent) inhibiting HSV-2 glycoprotein B. The different domains of gB are color-coded. Three domains are held together by the footprint of the nanobody. IMAGE: Benjamin Vollmer / Centre for Structural Systems Biology

Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de

t1p.de/cxt7a

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social

Optimal tilt-increment for cryo-ET Cryo-electron tomography (cryo-ET) enables high-resolution, three-dimensional imaging of cellular structures in their native, frozen state. However, image quality is limited by a trade-off between ang...

More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, Tomáš Majtner, Beata Turoňová, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

New luminescent animal unlocked!

Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...

Exclusive: Famed protein structure competition nears end as NIH grant money runs out Agency silent on funding renewal for contest that inspired creation of AIs that predicted how proteins would fold

Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.

Ana García-Sáez Awarded Prestigious ERC Grant

This is Ana’s third ERC grant! We’re proud to celebrate her success and groundbreaking work here at the Max Planck Institute of Biophysics. #ERCAdG Read more here 🔗 www.biophys.mpg.de/2872321/garcia-saez-awarded-erc-grant

Our latest research is out 📄 !

This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.

@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social

The electron cryo-microscope adapted for use at liquid nitrogen temperatures within its custom-made black box.

A colder frontier in cryo-EM 🧪🔬

Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).

Read more: tinyurl.com/mwwcunkc

#LMBResearch

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social

🔗 Preprint here tinyurl.com/3a74uanv

Look at those nucleosomes! 🧬 Impressive work on chromatin architecture from our colleagues, check it out below! ⬇️

Swinging lever mechanism of myosin directly shown by time-resolved cryo-EM - Nature A study using time-resolved cryogenic electron microscopy reveals the swinging lever mechanism of myosin, providing information on the molecular basis behind the production of force and movement by my...

Swinging lever mechanism of myosin shown by time-resolved cryoEM www.nature.com/articles/s41... so glad to finally be able to share this with the community! @astburycentre.bsky.social @astbury-bsl.bsky.social @stemuench.bsky.social @snmcmillan.bsky.social @davidklebl.bsky.social

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social

Interesting sample preparation method moving away from blotting

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

I will think of this paper next time I am there... 😀

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

Love this.. 👏

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony

www.biorxiv.org/content/10.1...

Wondering about phase separation and its impact in selective autophagy? Check the latest publication from the lab! ⬇️

In situ architecture of a nucleoid-associated biomolecular co-condensate that regulates bacterial cell division | PNAS In most bacteria, cell division depends on the tubulin-homolog FtsZ that polymerizes in a GTP-dependent manner to form the cytokinetic Z-ring at th...

Latest from Baumeister & @lottesogaard.bsky.social labs:

The PomXYZ complex and its role in bacterial cell division.

www.pnas.org/doi/10.1073/...

#teamtomo #cryoET #cryoEM

The 2025 EMBL Annual Poster, listing all of the courses and conferences taking place in 2025 at EMBL

Hi Bluesky community! 👋

Are you looking forward to 2025 as much as we are? 👀

We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!

➡️ s.embl.org/poster-bl

#EMBLEvents #molecularbiology #lifesciencetraining

Passage of the HIV capsid cracks the nuclear pore Upon infection, human immunodeficiency virus (HIV-1) releases its cone-shaped capsid into the cytoplasm of infected T-cells and macrophages. As its largest known cargo, the capsid enters the nuclear p...

Check out our latest preprint if you want to find out what happens when capsid and NPC interact (spoiler: it doesn't seem to end well for the NPC) #teamtomo
www.biorxiv.org/content/10.1...

Chaperonin TRiC within human cells. The left panel displays a representative 3D map from a tomogram illustrating the spatial distribution of open and closed TRiC complexes in human cells. The top right panel highlights the interaction of PDCD5 with the CCT3-1-4 subunits of the open TRiC complex. The bottom right panel shows the closed TRiC complex forming clusters of two particles.

Do not miss our latest #cryoET work! 🕵️ Xing et al. present the dynamic architecture of TRiC in human cells! The study of this protein-folding chaperonin opens the door to understanding its dysfunction in diseases like cancer and neurodegeneration 🧠🧪 #teamTOMO @becklab.bsky.social

Look at those cages 😍

A reindeer crafted from epoendorf tubes and pipette tips

A reindeer crafted from eppendorf tubes and pipette tips

Is this not the finest lab crafted Christmas tree decoration you’ve ever seen? Courtesy of Amy Tooke!